Cardiac fibrosis, a key contributor to ventricular pathologic remodeling and heart failure, currently lacks effective therapeutic approaches. Small extracellular vesicles from young healthy human plasma (Young-sEVs) were characterized via protein marker, transmission electron microscopy, nanoparticle tracking analysis, then applied in cellular models mouse of cardiac fibrosis. Western blotting qRT-PCR used identify protective signaling pathways fibroblasts (CFs). Young-sEVs significantly...

The acquired drug resistance would influence the efficacy of cisplatin-based chemotherapy in non-small-cell lung cancer. present study aimed to investigate correlation long non-coding RNA (lncRNA) H19 with cisplatin-resistance and clinical outcome adenocarcinoma. In our study, expression cisplatin-resistant A549/DDP cells was unregulated. Knockdown restored response cisplatin. H19-mediated chemosensitivity enhancement associated metastasis, induction G0/G1 cell-cycle arrest, cell...

Tumor metastasis is still the leading cause of melanoma mortality. Luteolin, a natural flavonoid, found in fruits, vegetables, and medicinal herbs. The pharmacological action mechanism luteolin on remain elusive. In this study, we investigated effect A375 B16‐F10 cell viability, migration, invasion, adhesion, tube formation human umbilical vein endothelial cells. Epithelial–mesenchymal transition (EMT) markers pivotal molecules HIF‐1α/VEGF signaling expression were analysed using western...

Multidrug resistance (MDR) generally leads to breast cancer treatment failure. The most common mechanism of MDR is the overexpression ATP-binding cassette (ABC) efflux transporters such as P-glycoprotein (P-gp) that reduce intracellular accumulation various chemotherapeutic agents. Celecoxib (CXB), a selective COX-2 inhibitor, can dramatically enhance cytotoxicity doxorubicin (DOX) in cells overexpressing P-gp. Thus it be seen combination DOX and CXB maybe obtain synergistic effects against...

Endometrial cancer (EC) is the fourth most common malignancy of female genital tract worldwide. MicroRNAs are important gene regulators with critical roles in diverse biological processes, including tumorigenesis. Several study’s show that miR-139-5p involved tumorigenesis and metastasis various cancers. However, its expression potential biologic role endometrial remain to be determined. This study aimed investigate analyze function underlying molecular mechanism cancer. Expression was...

Tissue culture techniques have been routinely used for banana propagation and offered rapid production of planting materials with favorable genotypes free pathogenic microorganisms in the industry. Meanwhile, extensive scientific work suggests that micropropagated plantlets are more susceptible to Fusarium oxysporum f. sp. cubense ( Foc ), deadly strain causes wilt bananas than conventional material due loss indigenous endophytes. In this study, an endophytic bacterium Bacillus velezensis...

The intracellular survival of M. tuberculosis (Mtb) plays a crucial role in its pathogenesis, which depends on various Mtb oxidoreductases that are resistant to reactive oxygen and nitrogen species (ROS RNS) generated by the host during infection. Secretory protein Rv1324 is potential virulence factor possible thioredoxin has oxidoreductase activity against ROS RNS

ABSTRACT Embryonic morphogenesis of a complex organism requires proper regulation patterning and directional growth. Planar cell polarity (PCP) signaling is emerging as crucial evolutionarily conserved mechanism whereby information conveyed. PCP thought to be established by global cues, recent studies have revealed an instructive role Wnt gradient in epithelial tissues both invertebrates vertebrates. However, it remains unclear whether Wnt/PCP regulated coordinated manner with embryonic...

Background: Breast cancer is a common malignancy in women. Conventional clinical therapies for breast all display moderate efficacies and limitations. It urgent to explore the novel combined therapeutic strategies meet demand. Methods: An iRGD tumor-penetrating peptide-modified nano-delivery system (denoted as [email protected] @JQ1/ORI nanoparticles) based on marine sulfated polysaccharide was developed by codelivery of JQ1 (BET inhibitor) oridonin (ORI, bioactive diterpenoid derived from...

Mesenchymal stem cell-derived extracellular vesicles (MSCs-EVs) possess cardioprotection in acute myocardial infarction. Nevertheless, the therapeutic intervention potential and molecular mechanism of EVs from NMN (Nicotinamide mononucleotide) preconditioned hUCMSCs (N-EVs) infarction remains unknown. In present study, (M-EVs) N-EVs were identified by electron microscopy, immunoblotting nanoparticle tracking analysis. Compared with M-EVs, significantly increased proliferation, migration,...

The basic leucine zipper (bZIP) transcription factor (TF) family performs diverse functions in fungal processes, including vegetative growth, nutrient utilization, stress responses, and invasion. Despite their importance, little is known about the bZIP members Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), a highly virulent banana pathogen. In this study, we systematically identified 17 bZIPs distributed across 10 Foc TR4 chromosomes classified them into four types based on...

Studies have suggested a possible correlation between the newly identified E3 ubiquitin ligase ring finger protein 146 (RNF146) and tumor development. However, until now, studies on RNF146 been restricted to poly(ADP-ribosyl)ation ligation, whereas role of in biology has rarely reported. In present study, non-small cell lung cancer (NSCLC) was investigated. The results showed that expression increased clinical samples lines. correlated with size, differentiation level, lymphatic metastasis,...

Phototherapies have many advantages for triple-negative breast cancer (TNBC) treatment. However, their effects are often limited by short blood circulation time, poor tumor selectivity and weak penetration of phototherapeutic agents, hypoxia. For overcoming these limitations, a versatile biomimetic system is developed based on red cells (RBCs). Photothermal agent new indocyanine green (IR820) conjugated with the cell/tissue-penetrating TAT peptide further efficiently encapsulated into intact...

Epithelial-mesenchymal transition (EMT) has been reported to play pivotal roles in tumor invasion and metastasis. Inhibition of EMT may exert beneficial effects regulating Oridonin (ORI), an active diterpenoid compound isolated from Rabdosia rubescens, was found be a potent anti-metastatic agent. However, the possible involvement ORI malignant melanoma is unclear. The present study that inhibited cell migration, invasion, adhesion A375 B16-F10 cells. transforming growth factor-β1...

Metastasis is the primary cause of mortality in patients with breast cancer and lacks effective therapeutic agents. Oridonin, an active diterpenoid compound isolated from Rabdosia rubescens, was identified to be most potent anti-tumor ingredient. However, molecular mechanisms responsible for its anti-metastatic effects remain unclear. In present study, oridonin significantly suppressed migration, invasion adhesion MDA-MB-231 4T1 cells, inhibited tube formation human umbilical vein...