Long non-coding RNAs (lncRNAs) are key players in the development and progression of human cancers. The lncRNA XIST (X-inactive specific transcript) has been shown to be upregulated non-small cell lung cancer (NSCLC); however, its role molecular mechanisms NSCLC remain unclear.qRT-PCR was conducted assess expression miR-186. Cell proliferation detected using MTT assay. invasion migration were evaluated transwell cycle distribution apoptosis rates analyzed by flow cytometry. Luciferase...

Hepatic cancer is a serious disease with high morbidity and mortality. Theranostic agents effective diagnostic therapeutic capability are highly needed for the treatment of hepatic cancer. Herein, we aimed to develop novel mesoporous polydopamine (MPDA)-based theranostic agent T1/T2 dual magnetic resonance imaging (MRI)-guided chemo-photothermal therapy. Superparamagnetic iron oxide (SPIO)-loaded MPDA NPs ([email protected]) was firstly prepared, followed by modifying targeted molecule...

BackgroundResistance to chemotherapeutic treatment is a common phenomenon in cancers, especially hepatocellular carcinoma (HCC). The Hippo signaling pathway has been demonstrated play role tumor initiation, development, and progression. However, little known about its roles the HCC chemoresistance.MethodsIn this study, real-time PCR western blotting were used identify expression profile of key components between chemoresistant chemosensitive cell lines. In vitro vivo loss- gain-of-function...

Mechanistic/mammalian target of rapamycin (mTOR) has emerged as a new potential therapeutic for gastric cancer. Rapamycin and analogs are undergoing clinical trials have produced responses in subgroup cancer patients. However, monotherapy with at safe dosage fails to induce cell apoptosis tumor regression which hampered its application. This led the exploration more effective combinatorial regimens enhance effectiveness rapamycin. In our present study, we investigated combination reactive...

Abstract Cancer stem cells (CSCs) are a subpopulation of within tumors that believed to possess pluripotent properties and thought be responsible for tumor initiation, progression, relapse metastasis. Cytoplasmic polyadenylation element-binding protein 1 (CPEB1), sequence-specific RNA-binding regulates mRNA translation, has been linked cancer progression However, the involvement CPEB1 in hepatocellular carcinoma (HCC) remains unclear. In this study, we have demonstrated directly sirtuin...

Alternative splicing within a gene can create different versions of an mRNA, called isoforms. CFIm, composed small subunit CFIm25 and two large subunits CFIm68 CFIm59 (also known as CPSF7), has been proposed enhancer-dependent activator mRNA 3' processing. In this study, we investigated the role CPSF7 in hepatocellular carcinoma. Experimental evidence suggests that expression level is higher liver cancer cells tissues than non-tumor hepatic tissues. Furthermore, knockdown effectively...

Hepatocellular carcinoma (HCC) is a highly lethal malignancy mostly because of metastasis, recurrence and acquired resistance to conventional chemotherapy. Arsenic trioxide (ATO) successfully used treat hematological malignancies, has been proven trigger apoptosis in HCC cells. However, the phase II trial evaluating efficacy toxicity ATO patients with showed that single-agent poorly active against HCC. Therefore, it great importance develop effective chemosensitization agents ATO. The aim...

Background: The present study aimed to explore the changes in hepatic metabolic profile during evolution of diabetes mellitus (DM) and verify key pathways. Methods: Liver samples were collected from diabetic rats induced by streptozotocin (STZ) control group at 1, 5, 9 weeks after STZ administration. Proton nuclear magnetic resonance spectroscopy (1H NMR)-based metabolomics was used examine DM, partial least squares-discriminate analysis (PLS-DA) performed identify metabolites. Results: We...

Tumor microenvironments are characterized by resistance to chemotherapeutic agents and radiotherapy. Hypoxia plays an important role in the development of tumor resistance, as well generation metastatic potential. YAP also participates regulation hypoxia-mediated chemoresistance, is negatively regulated protein tyrosine phosphatase non-receptor type 14 (PTPN14).The PTPN14 expression hepatocellular carcinoma (HCC) tissues were evaluated qRT-PCR, western blot tissue microarrays. The effect on...

Background: Epidemiological evidences regarding the association between physical activity and risk of lung cancer are still controversial. Objectives: We aimed to investigate relationship in men women, as well other high-risk populations such cigarette smokers. Methods: conducted a meta-analysis cohort studies evaluate cancer. Relevant were identified by searching PubMed Web Knowledge through August 2018. Study-specific relative (RR) with 95% confidence interval (CI) pooled using random...

Activated oncogenes and loss of tumor suppressors contribute to reprogrammed energy metabolism induce aerobic glycolysis, also known as Warburg effect. MicroRNAs are profoundly implicated in human malignancies by inhibiting translation multiple mRNA targets. Using hepatocellular carcinoma (HCC) molecular profiles from The Cancer Genome Atlas (TCGA), we identified a handful dysregulated microRNA HCC especially miR-34c-3p. Antagonization miR-34c-3p inhibited the lactate production, glucose...

The objective of this study was to assess the efficacy computed tomography (CT)-guided 125I brachytherapy alone in improving survival and quality life patients with unresectable locally advanced non-small-cell lung cancer (NSCLC) after one cycle first-line chemotherapy. Sixteen NSCLC were treated CT-guided chemotherapy (group A). who received only best supportive care B) matched up group A. Primary end point included survival, secondary assessment safety, effectiveness brachytherapy,...

Abstract Ubiquitin activating enzyme 2 (UBA2) is a basic component of E1‐activating in the SUMOylation system. Expression and function UBA2 human cancers are largely unknown. In this study we investigate expression non–small‐cell lung cancer. Immunochemistry showed that was overexpressed cancer tissues (53.3%, 40 75) compared with normal (14.3%, 4 28) ( P < 0.05). Immunostaining mainly detected nucleus. Overexpression significantly associated poor differentiation, large tumor size >...

In this study, with the aid of microarray technology, transmembrane protease serine 4 (TMPRSS4), a novel member family, was found to be upregulated in majority lung adenocarcinoma (LUAD) tissues compared normal tissues. Of note, clinical significance TMPRSS4 LUAD has not yet been reported, at least best our knowledge. Through immunohistochemistry assays, we that overexpressed and expression level also proportionally associated AJCC stage, T stage pathological grade. Moreover, high adverse...

Conditional survival rate (CSR) is defined as the dynamic possibility of survival, considering changes in risk over time. The present study aimed to compare CSR surgical procedures for stage IA1 non-small cell lung cancer (NSCLC). Overall, data 2,535 patients with NSCLC after lobectomy, segmentectomy or wedge resection were obtained from Surveillance, Epidemiology and End Results database, overall (OS) rates subsequently compared. estimates, who had already survived × years, survive further...

Abstract Background Accumulating evidences have been reported that long noncoding RNAs play crucial roles in the progression of hepatocellular carcinoma (HCC). SnoRNA host gene 6 (SNHG6) is believed to be involved several human cancers, but specific molecular mechanism SNHG6 HCC not well studied. Methods In this study, we experimentally down-regulated two cell lines vitro , and then measured proliferation, migration invasion abilities apoptotic levels. Also, performed xenograft assay...

Previous studies have indicated that lung adenocarcinoma (LUAD) is one of the common human malignancies, and its incidence keeps rising. With help microarray technology, downregulation miR-373-3p was observed in LUAD tissues compared with normal tissues. Notably, present study demonstrated expression amyloid precursor protein (APP) mRNA overexpressed adjacent Bioinformatic analysis may interact 3' untranslated region APP mRNA, then western blot dual-luciferase reporter gene assays were...

The systemic investigation of the metabolic pathways associated with hepatic tumorigenesis is important to discover novel biomarkers and identify potential pathogenesis. Here, 1 H nuclear magnetic resonance- ( NMR-) based metabolomic analysis was used monitor whole process rat diethylnitrosamine-induced HCC. Intraperitoneal administration diethylnitrosamine (DEN) induce primary HCCs in male Sprague-Dawley rats. Magnetic resonance imaging (MRI) examinations were performed follow tumor...

Abstract Thrombopoietin (TPO) is a haematopoietic cytokine mainly produced by the liver and kidneys, which stimulates production maturation of megakaryocytes. In past decade, numerous studies have investigated effects TPO outside system; however, role in progression solid cancer, particularly lung has not been well studied. Exogenous does affect non‐small‐cell cancer (NSCLC) cells as these show no or extremely low receptor expression; therefore, this study, we focused on endogenous NSCLC...

Lung cancer patients slated for surgery are at high risk of venous thromboembolism (VTE). Precise assessment is necessary providing proper thromboprophylaxis and reducing morbidity mortality VTE.A multicenter, observational, cross-sectional cohort study, involving with primary lung undergoing surgery, was carried out from August 2016 to December 2019. All were assessed according the Caprini model (RAM) a modified scoring system incorporating elevated D-dimer new stratification surgical time....

Genome-wide association studies (GWAS) have identified three loci at 18q21 (rs4939827, rs7240004, and rs7229639), which maps to SMAD7 loci, were associated with risk of diseases the digestive system. However, their associations hepatocellular carcinoma (HCC) remain unknown. A case-control study was conducted assess genetic HCC clinicopathologic development among Chinese Han population. Three SNPs genotyped 1,000 cases controls using Sequenom Mass-ARRAY technology. We observed statistically...

Abstract Zbed3, a BED finger domain‐containing protein was found to promote cancer proliferation by regulating β‐catenin expression through interacting with Axin. But whether and how domain function in is unknown. We constructed five mutants of which lacks the Axin‐Zbed3 binding site, 43 52, 69 77, 87 92, 97 104 sequences domain, respectively named them as Z‐A, Z1, Z2, Z3, Z4. Transfection both wild‐type Zbed3 Z4 ( P < 0.05), but not Z2 > 0.05) significantly upregulated NCI‐H1299...