A5060601648- Mast cells and histamine
- SARS-CoV-2 and COVID-19 Research
- Asthma and respiratory diseases
- Urticaria and Related Conditions
- COVID-19 Clinical Research Studies
- Allergic Rhinitis and Sensitization
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Food Allergy and Anaphylaxis Research
- Influenza Virus Research Studies
- Cancer, Hypoxia, and Metabolism
- Bacteriophages and microbial interactions
- Hops Chemistry and Applications
- Dermatology and Skin Diseases
- PARP inhibition in cancer therapy
- Biosensors and Analytical Detection
- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Phenothiazines and Benzothiazines Synthesis and Activities
- Metabolism, Diabetes, and Cancer
Xi'an Jiaotong University
2019-2024
Carnitine palmitoyltransferase 1A (CPT1A), which resides on the mitochondrial outer membrane, serves as rate-limiting enzyme of fatty acid β-oxidation. Identifying compounds targeting CPT1A warrants a promising candidate for modulating lipid metabolism. In this study, we developed CPT1A-overexpressed membrane chromatography (MMC) to screen with affinity CPT1A. Cells overexpressing were cultured, and subsequently, their was isolated immobilized amino-silica gel cross-linked by glutaraldehyde....
Membrane protein (MP)-based biomaterials have a wide range of applications in drug screening, antigen detection, and ligand–receptor interaction analysis. Traditional MP immobilization methods the disadvantage disordered orientation, leading to shielded binding domain unreliable pattern. Herein, we describe site-specific covalent MPs, which utilizes styrene maleic acid (SMA) detergent-free extraction method MPs as well reaction between His-tag divinyl sulfone (DVS). As an example, covalently...
Adverse effects that result from dexamethasone (DEX) use are common and serious in patients with asthma. Therefore, alternative anti‐inflammatory treatments being investigated. Isoimperatorin (ISO), an active natural furocoumarin, possesses multiple pharmacological properties, including anti‐inflammation effect. In this study, investigations were conducted on the effect of ISO mast cell (MC) activation vitro whether could reduce effective dose DEX a cell‐dependent murine model asthma vivo....
Anaphylaxis is a severe systemic allergic reaction that exhibits multiple clinical symptoms. The Mas-related G protein-coupled receptor X2 (MRGPRX2) recognized as key cell mediating diseases and drug-induced anaphylactoid reactions. Thus, it has been promising target for preventing treating these Based on the potential activity of imperatorin active structural feature MRGPRX2, we first demonstrated synthetic derivatives (IDs) could significantly inhibit MRGPRX2 agonist-induced degranulation...
Allergic diseases are a group of allergen-induced unfavorable immune responses initiating various symptoms in different organs. Mas-related G protein-coupled receptor X2 (MRGPRX2) on mast cells has been reported to be responsible for immunoglobulin E (IgE)-independent and allergic drug reactions therefore crucial target the development anti-pseudo-allergic agents. Considering active structural features MRGPRX2, we designed synthesized series diaryl ureas (DPUs). DPUs exert promising potency...
Background Clozapine is one of the most widely used second-generation antipsychotics in clinic. However, allergy-like symptoms such as rash and angioedema have been reported frequently, mechanism still not clear. Mas-related G protein-coupled receptor X2 (MRGPRX2) expressed on mast cells a crucial for drug induced pseudo-allergic reactions. Therefore, we explored whether by clozapine were associated with allergic reaction through MRGPRX2.Methods The effects reactions evaluated degranulation...
Three biphenyl furanocoumarin compounds have significant effects on inhibiting pseudovirus invasion into cells.
Abstract Fusion with host cell membrane is the main mechanism of infection SARS-CoV-2. Here, we propose a new strategy to double block SARS-CoV-2 fusion by using Harringtonine (HT), small-molecule antagonist. By chromatography (CMC), found that HT specifically targeted S protein and TMPRSS2, then confirmed can inhibit pseudotyped virus fusion. Furthermore, successfully blocked infection, especially in delta Omicron mutant. Since antagonist, it minimally affected continuous variation Our...
Abstract Background The novel coronavirus disease (2019-nCoV) has been affecting global health since the end of 2019 and there is no sign that epidemic abating. major issue for controlling infectious lacking efficient prevention therapeutic approaches. Chloroquine (CQ) Hydroxychloroquine (HCQ) have reported to treat disease, but underlying mechanism remains controversial. Purpose objective this study investigate whether CQ HCQ could be ACE2 blockers used inhibit 2019-nCoV virus infection....
Mast cells (MCs) are innate immune that can cause allergic reactions.
The Corona Virus Disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), is a highly contagious disease that has posed significant threat to global public health safety. Effective anti-drugs are urgently needed. In this study, based on the membrane chromatography (CMC) model of HEK293T overexpression angiotensin-converting enzyme (ACE2), six biphenyl furanocoumarin compounds with long retention time ACE2h-CMC have been screened from synthesized...