A5022546357- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- 3D Printing in Biomedical Research
- Biocrusts and Microbial Ecology
- Force Microscopy Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Skin and Cellular Biology Research
- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cell Image Analysis Techniques
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Plant Reproductive Biology
- Galectins and Cancer Biology
- Animal Genetics and Reproduction
- Microtubule and mitosis dynamics
- Developmental Biology and Gene Regulation
- Metabolism and Genetic Disorders
- Neurobiology and Insect Physiology Research
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Chromatin Dynamics
- Functional Brain Connectivity Studies
- ATP Synthase and ATPases Research
- Nanocluster Synthesis and Applications
University of Zurich
2018-2024
Université Grenoble Alpes
2010-2020
Centre National de la Recherche Scientifique
2001-2019
CEA Grenoble
2007-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2007-2019
Laboratoire de Physiologie Cellulaire
2009-2019
Biotechnology Institute
2019
Laboratoire Physiologie Cellulaire & Végétale
2006-2017
Université Joseph Fourier
2007-2017
Institut de Recherches en Technologies et Sciences pour le Vivant
2007-2017
The organization of actin filaments into higher-ordered structures governs eukaryotic cell shape and movement. Global network size architecture are maintained in a dynamic steady state through regulated assembly disassembly. Here, we used experimentally defined vitro to investigate how the activity myosin motors depends on architecture. Direct visualization revealed myosin-induced deformation. During this reorganization, myosins selectively contracted disassembled antiparallel structures,...
Abstract Intermediate filaments (IFs) are integral components of the cytoskeleton. They provide cells with tissue-specific mechanical properties and involved in numerous cellular processes. Due to their intricate architecture, a 3D structure IFs has remained elusive. Here we use cryo-focused ion-beam milling, cryo-electron microscopy tomography obtain vimentin (VIFs). VIFs assemble into modular, intertwined flexible helical 40 α-helices cross-section, organized five protofibrils....
Proteins of the Wiskott-Aldrich Syndrome protein (WASp) family connect signaling pathways to actin polymerization-driven cell motility. The ubiquitous homolog WASp, N-WASp, is a multidomain that interacts with Arp2/3 complex and G-actin via its C-terminal WA domain stimulate polymerization. activity N-WASp enhanced by binding effectors like Cdc42-guanosine 5′-3-<i>O</i>-(thio)triphosphate, phosphatidylinositol bisphosphate, or <i>Shigella</i> IcsA protein. Here we show SH3-SH2-SH3 adaptor...
The extremities of chromosomes end in a G-rich single-stranded overhang that has been implicated the onset replicative senescence. repeated sequence forming G-overhang is able to adopt peculiar four-stranded DNA structure vitro called G-quadruplex, which poor substrate for telomerase. Small molecule ligands selectively stabilize telomeric G-quadruplex induce telomere shortening and delayed growth arrest. Here we show ligand telomestatin dramatic effect on conformation intracellular...
Vinculin plays a fundamental role in integrin-mediated cell adhesion. Activated by talin, it interacts with diverse adhesome components, enabling mechanical coupling between the actin cytoskeleton and extracellular matrix. Here we studied interactions of activated full-length vinculin way regulates organization dynamics Arp2/3 complex-mediated branched network. Through combination surface patterning light microscopy experiments show that can bundle dendritic networks through rapid binding...
Abstract Intermediate filaments (IFs) are integral components of the cytoskeleton. They provide cells with tissue-specific mechanical properties and involved in numerous cellular processes. Due to their intricate architecture, a 3D structure IFs has remained elusive. Here we use cryo-focused ion beam milling, cryo-electron microscopy tomography, obtain vimentin (VIFs). VIFs assemble into modular, densely-packed highly-ordered helical symmetric 40 α-helices cross-section, organized 5...
The talin-vinculin axis is a key mechanosensing component of cellular focal adhesions. How talin and vinculin respond to forces regulate one another remains unclear. By combining single-molecule magnetic tweezers experiments, Molecular Dynamics simulations, actin-bundling assays, adhesion assembly experiments in live cells, we here describe two-ways allosteric network within as regulator the interaction. We directly observe maturation process upon binding, which reinforces binding at rate...
Actin-based propulsion of the bacteria Listeria and Shigella mimics forward movement leading edge motile cells. While harnesses eukaryotic protein N-WASp to stimulate actin polymerization filament branching through Arp2/3 complex, surface ActA directly activates complex by an unknown mechanism. Here we show that N-terminal domain binds one monomer, in a profilin-like fashion, C-terminal WASp family proteins catalyzing barbed end complex. No evidence is found for side filaments ActA-activated...
Human telomeric DNA consists of tandem repeats the sequence d(TTAGGG) with a 3′ single-stranded extension (the G-overhang). The stabilization G-quadruplexes in human by small-molecule ligands inhibits activity telomerase and results telomere uncapping, leading to senescence or apoptosis tumor cells. Therefore, search for new selective G-quadruplex is considerable interest because ligand might provide telomere-targeted therapeutic approach treatment cancer. We have screened bank derivatives...
Cell motility depends on the rapid assembly, aging, severing, and disassembly of actin filaments in spatially distinct zones. How a set regulatory proteins that sustains actin-based force generation during work together space time remains poorly understood. We present our study distribution dynamics Arp2/3 complex, capping protein (CP), actin-depolymerizing factor (ADF)/cofilin "comet tails," using minimal reconstituted system with nucleation-promoting (NPF)-coated beads. The complex...
Cells use their dynamic actin network to control mechanics and motility. These networks are made of branched filaments generated by the Arp2/3 complex. Here we study under which conditions microscopic organization builds up a sufficient stress trigger sustained In our experimental setup, or “gels” grown on hard bead in controlled minimal protein system containing monomers, profilin, complex capping protein. We vary concentrations follow experimentally through simulations shape mechanical...
Abstract The growth of branched actin networks powers cell-edge protrusions and motility. A heterogeneous density actin, which yields to a tunable cellular response, characterizes these dynamic structures. We study how organization controls both the rate steering during lamellipodium growth. use high-resolution surface structuration assay combined with mathematical modeling describe reconstituted lamellipodium. demonstrate that local monomer depletion at site assembly negatively impacts...
Abstract In the cell nucleus, chromatin is anchored to nuclear lamina, a network of lamin filaments and binding proteins that underly inner membrane. The lamina involved in organisation through interaction lamina-associated domains (LADs) within densely packed heterochromatin regions. Employing cryo-focused ion beam (cryo-FIB) milling conjunction with cryo-electron tomography (cryo-ET), we analysed distribution nucleosomes at lamin-chromatin interface. Depletion A/C reduced concentration...