Abstract Background Recent research increasingly highlights a strong correlation between gut microbiota and the risk of gastrointestinal diseases. However, whether this relationship is causal or merely coincidental remains uncertain. To address this, Mendelian randomization (MR) analysis was undertaken to explore connections prevalent Methods Genome-wide association study (GWAS) summary statistics for microbiota, encompassing diverse range 211 taxa (131 genera, 35 families, 20 orders, 16...

Abstract With the aging of population, sarcopenia has become more common. Studies have shown a broad association between liver disease and sarcopenia. However, this link remains unclear. Our study explored NAFLD predicting pathogenesis. To begin, we investigated causal relationship genetic correlation them using MR LDSC. Second, each GWAS was annotated by MAGMA. The genes were analyzed for pleiotropy PLACO approach. Finally, functional analysis conducted on identified pleiotropic genes. We...

Abstract Cerebral amyloid β-peptide (Aβ) accumulation resulting from an imbalance between Aβ production and clearance is one of the most important causes in formation Alzheimer’s disease (AD). In order to preserve maintenance homeostasis have a notable AD therapy, achieving method clear up plaques becomes emerging task. Herein, we describe self-destructive nanosweeper based on multifunctional peptide-polymers that capable capturing clearing for effective treatment AD. The recognize bind via...

Previous observational studies have indicated that gut microbiota and metabolites may contribute to heart failure its risk factors. However, with the limitation of reverse causality confounder in studies, such relationship remains unclear. This study aims reveal causal effect on factors.This collected summary statistics regarding metabolites, failure, diabetes, hypertension, chronic kidney disease, myocardial infarction, atrial fibrillation, hypertrophic cardiomyopathy, dilated coronary...

Atherosclerosis (AS) is the most common type in cardiovascular disease. Due to its complex pathogenesis, exact etiology of AS unclear. circRNA has been shown play an essential role diseases. However, underlying mechanism not understood clearly.Quantitative Real-Time PCR assay was used detect expression circRSF1, miR-135b-5p and histone deacetylase 1 (HDAC1). Western blot applied measure protein HDAC1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax), cleaved-caspase-3, vascular cell...

Although many studies have indicated that Psoriasis (PsO) could contribute to the risk of lung cancer, no study has reported a clear causal association between them. Our aim was explore potential PsO and cancer using Mendelian randomization (MR) design.To we used large-scale genetic summary data from genome-wide (GWAS), including (n = 337 159) 361 586), based on previous observational studies. main analyses were conducted by inverse-variance weighted (IVW) method with random-effects model,...

Metastatic castration-resistant prostate cancer (mCRPC) is one of the main contributors to death patients. To date, detailed molecular mechanisms underlying mCRPC are unclear. Given crucial role epithelial–mesenchymal transition (EMT) in metastasis, we aimed analyse expression and function Transforming growth factor-beta (TGF-β) signal-associated protein named Sox5 mCRPC. The levels were analysed by western blot, immunohistochemistry immunofluorescence. Luciferase reporter assays chromatin...

Atherosclerosis (AS) is the main cause of death in individuals with cardiovascular and cerebrovascular diseases. A growing body evidence suggests that oxidative stress plays an essential role pathology. The aim this study was to determine genetic mechanisms associated stress, as well construct a diagnostic model investigate its immune microenvironment. Seventeen stress-related genes were identified. four-gene constructed using least absolute shrinkage selection operator (LASSO) algorithm...

Objective Previous studies have demonstrated that trimethylamine N-oxide (TMAO) and its precursors, including choline, betaine, carnitine, are closely associated with blood pressure (BP) changes. Nevertheless, the limitation of reverse causality confounder in observational studies, such a relationship remains unclear. We aimed to assess causal TMAO precursors BP by Mendelian Randomization (MR) approach. Method In this study, two-sample MR was used reveal effect on BP. Pooled data from...

Abstract In the cell nucleus, chromatin is anchored to nuclear lamina, a network of lamin filaments and binding proteins that underly inner membrane. The lamina involved in organisation through interaction lamina-associated domains (LADs) within densely packed heterochromatin regions. Employing cryo-focused ion beam (cryo-FIB) milling conjunction with cryo-electron tomography (cryo-ET), we analysed distribution nucleosomes at lamin-chromatin interface. Depletion A/C reduced concentration...

With the development of blue laser endoscopy (BLE) technique, it's often used to diagnose early gastric cancer (EGC) by morphological changes blood vessels through BLE. However, EGC is still not obvious identify, resulting in a high rate missed diagnosis. Molecular imaging can show tumors at molecular level, which provides possibility for diagnosing EGC. Therefore, developing probe that visually monitors under BLE particularly necessary. Herein, bis-pyrene (BP) based nanoprobe (BP-FFVLK-(PEG)-RGD, M

Recent data have shown that γδ T cells can act as mediators for immune defense against tumors. Our previous study has demonstrated persisting clonally expanded TRDV4 might be relatively beneficial the outcome of patients with cell acute lymphoblastic leukemia after hematopoietic stem transplantation (HSCT). However, little is known about distribution and clonality TRDV repertoire in receptor (TCR) their effects on clinical myeloid (AML). The aim this was to assess whether oligoclonal...

High human telomerase reverse transcriptase (hTERT) expression is related to severe Colorectal Cancer (CRC) progression and negatively CRC patient survival. Previous studies have revealed that hTERT can reduce cancer cellular reactive oxygen species (ROS) levels accelerate progression; however, the mechanism remains poorly understood. NFE2-related factor 2 (NRF2) a molecule plays significant role in regulating ROS homeostasis, but whether there correlation between NRF2 unclear. Here, we...

Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant tumors and approximately 5% patients with chronic pancreatitis (CP) inevitably develop PDAC. This study aims explore key gene regulation involved in progression CP to PDAC, a particular emphasis on function lncRNAs.A total 103 pancreatic tissue samples collected from 11 92 respectively, were included this study. After normalizing logarithmically converting original data, differentially expressed lncRNAs (DElncRNAs) mRNAs...

Objectives The relationship between rheumatoid arthritis (RA) and coronary atherosclerosis is widely concerned, but observational studies have not clarified causality. We performed two-sample Mendelian randomization (MR) study to assess the causal association RA atherosclerosis. Methods we mainly conducted MR analysis using inverse variance weighted (IVW) approach. Weighted median, MR-Egger regression maximum likelihood were as sensitivity analyses for supplementary analysis. Multivariate...

During the development of shale gas, various issues such as low individual well production, rapid decline, limited reservoir control, and recovery rates have arisen. Enhancing gas has consistently been a focal point challenge within industry. Therefore, this paper employs molecular dynamic (MD) simulation methods to study adsorption diffusion characteristics CH4/CO2 at different temperatures mixing ratios. It compares effects temperature molar ratio changes on selectivity coefficient,...

The combination of different agents used for procedural sedation allows a greater range desirable effects while minimizing side effects. ketamine-dexmedetomidine (ketadex) and ketamine-propofol (ketofol) are successful examples. purpose this meta-analysis was to compare the safety efficacy ketadex with ketofol in pediatric patients.We searched Pubmed, Cochrane Controlled Register Trials, Embase from inception June 2022. Studies were independently evaluated inclusion criteria exclusion by two...

3,3′-Diindolylmethane (DIM) has been extensively studied as a potential therapeutic drug with free radical scavenging, antioxidant and anti-angiogenic effects. However, whether DIM similar effects on cardiomyocytes remains unknown. Here we evaluated DIM's influence inflammation apoptosis of H9C2 induced by LPS to explore the possible mechanism cells were incubated (10, 20 30 μM) or without for 24 h. The cytotoxicity was detected CCK-8. levels tumour necrosis factor (TNF)-α interleukin (IL)-6...