A5087084011- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Parkinson's Disease Mechanisms and Treatments
- Nicotinic Acetylcholine Receptors Study
- Neurological disorders and treatments
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Nerve injury and regeneration
- Memory and Neural Mechanisms
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipose Tissue and Metabolism
- Medicinal Plants and Neuroprotection
- Electrochemical sensors and biosensors
- Nuclear Receptors and Signaling
- Electrochemical Analysis and Applications
- Cholinesterase and Neurodegenerative Diseases
- Neurogenesis and neuroplasticity mechanisms
- Autism Spectrum Disorder Research
- Genetic Neurodegenerative Diseases
- Cellular transport and secretion
- Virus-based gene therapy research
- Olfactory and Sensory Function Studies
University of Oxford
2016-2025
Parkinson's UK
2010-2025
Research Network (United States)
2023-2025
Molecular Devices (United Kingdom)
2023
Pediatrics and Genetics
2010-2016
John Radcliffe Hospital
2012
RELX Group (United Kingdom)
2006
Mansfield University
2004-2006
Dopamine (DA) transmission is governed by processes that regulate release from axonal boutons in the forebrain and somatodendritic compartment midbrain, clearance DA transporter, diffusion, extracellular metabolism. We review how regulated neuronal activity autoreceptors heteroreceptors, address quantal events are size frequency. In brain regions densely innervated axons, due predominantly to uptake whereas cortex, other with relatively sparse inputs, norepinephrine transporter diffusion...
Significance Elevated expression of the presynaptic protein α-synuclein underlies familial and sporadic Parkinson disease (PD). However, our understanding how increases in levels drive sequence events leading to PD is incomplete. Here, we apply a multidisciplinary longitudinal analysis new transgenic mouse model. We show that early-stage decreases dopamine release vesicle reclustering precede late-stage changes neuronal firing properties, measured by vivo recordings from vulnerable neurons....
Significance Deciphering the roles of midbrain dopaminergic neurons in control movement is critical not only for understanding normal motor function but also defining basis dysfunction Parkinson’s disease. However activity these generally has been considered to be unrelated movement. Here we demonstrate that signal onset spontaneous a cell-type–selective manner and signals can read out transmitter receptor dynamics striatum, one their principal targets. Importantly, movement-related were...
This collection contains seven protocols detailing methods used in Zhang, Y. et al. (2025) An axonal brake on striatal dopamine output by cholinergic interneurons.
Abstract Studies in brain slices have provided a wealth of data on the basic features neurons and synapses. In intact brain, these properties may be strongly influenced by ongoing network activity. Although physiologically realistic patterns activity been successfully induced maintained interface‐type recording chambers, they harder to obtain submerged‐type which offer significant experimental advantages, including fast exchange pharmacological agents, visually guided patch‐clamp recordings,...
Striatal dopamine (DA) and acetylcholine (ACh) regulate motivated behaviors striatal plasticity. Interactions between these neurotransmitters may be important, through synchronous changes in parent neuron activities reciprocal presynaptic regulation of release. How DA signaling is regulated by muscarinic receptors (mAChRs) unresolved; contradictory reports indicate suppression or facilitation, implicating several mAChR subtypes on various neurons. We investigated whether varies with activity...
Dopamine (DA) is released from somatodendritic sites of neurons in the substantia nigra pars compacta (SNc) and ventral tegmental area (VTA), where it has neuromodulatory effects. The aim this study was to evaluate role D 2 autoreceptor inhibition regulation release each region. Fast cyclic voltammetry at carbon fiber microelectrodes used measure electrically evoked DA vitro . Furthermore, we compared with more familiar axon terminal caudate putamen (CPu) nucleus accumbens (NAc). Evoked...
The synucleins (α, β, and γ) are highly homologous proteins thought to play a role in regulating neurotransmission found abundantly presynaptic terminals. To overcome functional overlap between synuclein understand their signaling from mesostriatal dopaminergic neurons, we produced mice lacking all three members of the family. effect on system was assessed adult (4- 14-month-old) animals using combination behavioral, biochemical, histological, electrochemical techniques. Adult...
Mesostriatal dopaminergic neurons and striatal cholinergic interneurons participate in signaling the motivational significance of environmental stimuli regulate plasticity. Dopamine (DA) acetylcholine (ACh) have potent interactions within striatum at multiple levels that include presynaptic regulation neurotransmitter release postsynaptic effects target cells (including ACh neurons). These may be highly variable given dynamic changes firing activities parent DA neurons. Here, we consider how...
Abstract Alpha‐synuclein is intimately involved in the pathogenesis of Parkinson's disease, and has been implicated regulation synthesis, release reuptake dopamine (DA). However, mice lacking members synuclein family have reported to display no overt behavioural phenotype. This may be a result compensatory upregulation other synucleins during development. Here we report on behaviour DA synapse function alpha‐synuclein null, gamma‐synuclein alpha‐gamma‐synuclein double‐null knockout mice....
Nicotine is the primary psychoactive component of tobacco. Its reinforcing and addictive properties depend on nicotinic acetylcholine receptors (nAChRs) located within mesolimbic axis originating in ventral tegmental area (VTA). The roles oligomeric assembly subunit α4- α6-containing nAChRs dopaminergic (DAergic) neurons are much debated. Using subunit-specific knockout mice targeted lentiviral re-expression, we have determined dependence intracranial nicotine self-administration (ICSA) into...