A5076385340- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Marine Toxins and Detection Methods
- Genetically Modified Organisms Research
- Marine and coastal ecosystems
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Animal Virus Infections Studies
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Plant Molecular Biology Research
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Plant Gene Expression Analysis
- Insect Resistance and Genetics
- Environmental DNA in Biodiversity Studies
- Viral Infections and Vectors
- Plant tissue culture and regeneration
- SARS-CoV-2 and COVID-19 Research
- Birth, Development, and Health
Nofima
2023-2025
Norwegian Institute of Bioeconomy Research
2019-2025
Fafo Foundation
2023-2024
Norwegian University of Life Sciences
2017-2024
Norwegian Institute of Public Health
2020-2023
Norwegian Veterinary Institute
2006-2017
Oslo University Hospital
2017
Swedish Veterinary Agency
2007-2009
University of Oslo
2000-2006
Dana-Farber Cancer Institute
2004-2006
The three anomalously pigmented dinoflagellates Gymnodinium galatheanum, Gyrodinium aureolum, and breve have plastids possessing 19′-hexanoyloxy-fucoxanthin as the major carotenoid rather than peridinin, which is characteristic of majority dinoflagellates. Analyses SSU rDNA from plastid nuclear genome these dinoflagellate species indicate that they acquired their via endosymbiosis a haptophyte. sequences appear to undergone rapid sequence evolution, there considerable divergence between...
ABSTRACT Pfiesteria complex species are heterotrophic and mixotrophic dinoflagellates that have been recognized as harmful algal bloom associated with adverse fish human health effects along the East Coast of North America, particularly in its largest (Chesapeake Bay Maryland) second (Albermarle-Pamlico Sound Carolina) estuaries. In response to impacts on economy, monitoring programs detect organism implemented affected areas. However, until recently, specific identification two toxic known...
Seven bacterial isolates from farmed Atlantic cod displaying chronic granulomatous disease were characterized by phenotypic and molecular taxonomic methods. The Gram-negative, facultatively intracellular, non-motile, strictly aerobic coccobacilli which produced H(2)S cysteine-supplemented media are therefore phenotypically consistent with members of the genus Francisella. Comparison 16S rRNA gene sequences six partial housekeeping (groEL, shdA, rpoB, rpoA, pgm atpA) confirmed organism as a...
Tick-borne encephalitis (TBE) is among the most important vector borne diseases of humans in Europe and currently identified as a major health problem many countries. TBE endemic zones have expanded over past two decades, well number reported cases within areas. Multiple factors are ascribed for increased incidence TBE, including climatic change. The has also Norway decade, human cluster along southern coast Norway. In distribution prevalence virus (TBEV) tick populations largely unknown....
Approximately 15% of primary colorectal cancers have DNA mismatch repair deficiency, causing a complex genome with thousands small mutations-the microsatellite instability (MSI) phenotype. We investigated molecular heterogeneity and tumor immunogenicity in relation to clinical endpoints within this distinct subtype cancers.
The newly described heterotrophic estuarine dinoflagellate Pfiesteria piscicida has been linked with fish kills in field and laboratory settings, a novel clinical syndrome of impaired cognition memory disturbance among humans after presumptive toxin exposure. As result, there is pressing need to better characterize the organism these associations. Advances research have hampered, however, by absence genomic sequence data. We employed sequencing strategy directed heteroduplex mobility assay...
Myrionecta rubra (Lohmann 1908, Jankowski 1976 ) is a photosynthetic ciliate with global distribution in neritic and estuarine habitats has long been recognized to possess organelles of cryptophycean origin. Here we show, using nucleomorph (Nm) small subunit rRNA gene sequence data, quantitative PCR, pigment absorption scans, that an M. culture plastids identical those its cryptophyte prey, Geminigera cf. cryophila (Taylor Lee 1971, Hill 1991). Using demonstrate G . undergo division growing...
Species within the class Raphidophyceae were associated with fish kill events in Japanese, European, Canadian, and U.S. coastal waters. Fish mortality was attributable to gill damage exposure reactive oxygen species (peroxide, superoxide, hydroxide radicals), neurotoxins, physical clogging, hemolytic substances. Morphological identification of these organisms environmental water samples is difficult, particularly when fixatives are used. Because this difficulty continued global emergence...
The genetic element s2m seems to represent one of very few examples mobile elements in viruses. function remains obscure and a scattered taxonomical distribution has been reported by numerous groups. We have searched GenBank order identify all viral accessions that s2m(−like) sequence motifs. Rigorous phylogenetic analyses constrained tree topology testing were also performed investigate the apparently nature s2m. stem-loop structure can be found four families + ssRNA viruses; Astroviridae,...
Abstract In response to various stimuli, plants acquire resistance against pests and/or pathogens. Such acquired or induced allows rapidly adapt their environment. Spraying the bark of mature Norway spruce ( Picea abies ) trees with phytohormone methyl jasmonate (MeJA) enhances tree‐killing beetles and associated phytopathogenic fungi. Analysis chemical defenses beetle colonization success suggests that MeJA treatment both directly induces immune responses primes inducible for a faster...
There is compelling evidence from transgenic mouse studies and analysis of mutations in human carcinomas indicating that the TGF-β signal transduction pathway tumor suppressive.We have shown overexpression TGF-β1 mammary epithelial cells suppresses development expression a dominant negative type II receptor (DNIIR) under control MMTV promoter/enhancer increases incidence carcinomas.Studies tumors demonstrated inactivating genes encoding proteins involved transduction, including DPC4/Smad4,...
The Norwegian muskox (Ovibos moschatus) population lives on the high mountain plateau of Dovre and originates from animals introduced Greenland. In late summers 2006 2012, severe outbreaks pneumonia with mortality rates 25-30% occurred. During 2012 epidemic quality samples culled sick were obtained for microbiological pathological examinations. High throughput sequencing (pyrosequencing) pneumonic lung tissue revealed concentrations Mycoplasma ovipneumoniae in all six examined by this method...
Coronaviruses infect a wide variety of host species, resulting in range diseases both humans and animals. The coronavirus genome consists large positive-sense single-stranded molecule RNA containing many structures. One structure, denoted s2m consisting 41 nucleotides, is located within the 3' untranslated region (3' UTR) shared between some including infectious bronchitis virus (IBV), severe acute respiratory syndrome (SARS-CoV), SARS-CoV-2, as well other pathogens, human astrovirus. Using...
Hydrogen sulphide (H2S) is known to regulate various physiological processes, but its role in fish cardiac function, especially at the molecular level, poorly understood. This study examined functions of exogenous H2S, using sodium hydrosulphide (NaHS) as a donor, on Atlantic salmon cardiomyocytes. NaHS concentrations 10 160 μM showed limited cytotoxicity and no impact cell proliferation, though higher doses increased ATP activity. Menadione administered separately or sequentially...
Abstract The phenylpropanoid pathway, regulated by transcription factors of the MYB family, produces secondary metabolites that play important roles in fertilization and early phase fruit development. MYB46 factor is a key regulator cell wall structure, lignin flavonoid biosynthesis many plants, but little known about its activity flowers berries F. vesca . For functional analysis FvMYB46, we designed CRISPR-Cas9 construct with an endogenous -specific U6 promoter for efficient specific...
We have tested and compared the performance of 12 different assays representing four real-time polymerase chain reaction (PCR) chemistries in context genetically modified organism detection. Several molecular beacon, SYBR Green, TaqMan, MGB were designed for event specific detection quantification 3‘ integration junction GTS 40-3-2 (Roundup Ready) soybean. Sensitivity as well robustness presence background DNA tested. None PCR-based approaches appeared to be significantly better than any...
An epigenetic memory of the temperature sum experienced during embryogenesis is part climatic adaptation strategy long-lived gymnosperm Norway spruce. This has a lasting effect on timing bud phenology and frost tolerance in resulting epitype trees. The well characterized phenotypically at transcriptome level, but to what extent DNA methylation changes are involved have not previously been determined. To address this, we analyzed somatic embryos spruce clones produced contrasting...
Serial transfer of plastids from one eukaryotic host to another is the key process involved in evolution secondhand plastids. Such transfers drastically change environment and hence selection regimes, presumably leading changes over time characteristics plastid gene misleading phylogenetic inferences. About half dinoflagellate protists species are photosynthetic unique harboring a diversity acquired wide range algae. They therefore ideal for studying evolutionary processes gained through...