A5076804292- Cancer Research and Treatments
- Antimicrobial Peptides and Activities
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Telomeres, Telomerase, and Senescence
- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Cell death mechanisms and regulation
- Polydiacetylene-based materials and applications
- Biosensors and Analytical Detection
- Synthesis and biological activity
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Fermentation and Sensory Analysis
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Nutrition, Genetics, and Disease
- Computational Drug Discovery Methods
- Cancer Cells and Metastasis
- Immune Response and Inflammation
Hubei University of Technology
2025
Harbin Institute of Technology
2021
Guangzhou University of Chinese Medicine
2016-2020
Harbin Medical University
2019
Yunnan Institute of Parasitic Diseases
2019
National Institute for Parasitic Diseases
2019
Hospital for Tropical Diseases
2019
National Science and Technology Council
2019
Sichuan University
2008-2009
Hebei University of Science and Technology
2007-2008
Abstract Human α-defensin-1 (HNP1), a small antimicrobial peptide, shows cytotoxicity to tumor cells in vitro and inhibitory activity for pathologic neovascularization vivo. Here, we did gene therapy with plasmid that expresses secretable form of HNP1 assaying its antitumor activity. The expression secretion were determined by reverse transcription-PCR ELISA vitro. We found A549 caused significant growth inhibition. This effect is most likely cell autonomous, as amount recombinant protein...
Abstract Purpose: Human neutrophil peptides (HNP1-3), small molecular antimicrobial peptides, are expressed within tumors and associated with tumor necrosis inhibition of angiogenesis. Recent investigations have suggested that HNP1-3 likely to be involved in the host immune responses tumors. Experimental Design: We used recombinant pSec-HNP1, which expresses a secretable form HNP1, obtain expression HNP1 milieu immunocompetent mice explore possible roles immunity. The antitumor effects were...
// Hongbo Chen 1, 2, 6, * , Liqiao Han 3, Hsiangi Tsai Zhiwei Wang 4 Yanping Wu 2 Yanhong Duo 1 Wei Cao Lijun 5 Zhirong Tan 3 Ning Xu Xianzhang Huang Junhua Zhuang Laiqiang The Shenzhen Key Lab of Gene and Antibody Therapy, Center for Biotechnology & Biomedicine, Division Life Health Sciences, Graduate School at Shenzhen, Tsinghua University, 518055, China Beijing 100084, Department Laboratory Science, Second Affiliated Hospital Guangzhou University Chinese Medicine, 510120, Fourth Medical...
The analysis of intracellular ATP can reveal the response cells to different treatments and is important for individualized medicine. In present study, we developed a cell penetrating peptides (CPPs) tagged luciferase (TAT-LUC) tumor chemosensitivity assay. activity recombinant TAT-LUC was evaluated using standard solution cells. This then used sensitivity index (SI) four strains results showed that could detect less than 10 nM extracellular with strong correlation between luminescence...
Abstract Diosmin is able to exert anti-cancer effects on various cancer cells, including Caco-2 and HT-29. Herein, we set out investigate the action mechanisms of diosmin in HepG2 cell line. We utilized PharMapper server Genecards database identify target proteins. The Autodock software was used dock key targets with sites. MTT Western blot assays were determine proliferation, as well expression related proteins order validate antitumor effect diosmin. Results demonstrated that 13 14 tumor...
This is the protocol for Nested PCR assay