A5077907453- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Cardiovascular and exercise physiology
- Sports Performance and Training
- Diet and metabolism studies
- Exercise and Physiological Responses
- Physical Activity and Health
- Obesity, Physical Activity, Diet
- Adipokines, Inflammation, and Metabolic Diseases
- Nutrition and Health in Aging
- Heart Rate Variability and Autonomic Control
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Sports injuries and prevention
- Cardiovascular Effects of Exercise
- Muscle Physiology and Disorders
- Coenzyme Q10 studies and effects
- Non-Invasive Vital Sign Monitoring
- Regulation of Appetite and Obesity
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cardiovascular Disease and Adiposity
- Nutritional Studies and Diet
- Body Composition Measurement Techniques
- Bone health and osteoporosis research
- Pancreatic function and diabetes
University of Copenhagen
2016-2025
Medical University of Białystok
2023
Maersk (Denmark)
2021
Rigshospitalet
2000-2019
John Wiley & Sons (United States)
2017
Obesity Society
2017
Association on Higher Education And Disability
2017
Bispebjerg Hospital
2009-2016
Karolinska University Hospital
2016
Healthy Start
2016
Skeletal muscle mitochondrial content varies extensively between human subjects. Biochemical measures of proteins, enzyme activities and lipids are often used as markers oxidative capacity (OXPHOS). The purpose this study was to determine how closely associated these commonly biochemical OXPHOS. Sixteen young healthy male subjects were recruited for study. Subjects completed a graded exercise test maximal oxygen uptake (VO2peak) biopsies obtained from the vastus lateralis. Mitochondrial...
Obesity is associated with low-grade inflammation, insulin resistance, type 2 diabetes, and cardiovascular disease. This study investigated the effect of a 15-wk lifestyle intervention (hypocaloric diet daily exercise) on inflammatory markers in plasma, adipose tissue (AT), skeletal muscle (SM) 27 severely obese subjects (mean body mass index: 45.8 kg/m2). Plasma samples, subcutaneous abdominal AT biopsies, vastus lateralis SM biopsies were obtained before after analyzed by ELISA RT-PCR. The...
MicroRNAs (miRNA), small noncoding RNA molecules, may regulate protein synthesis, while resistance exercise training (RT) is an efficient strategy for stimulating muscle synthesis in vivo. However, RT increases mass, with a very wide range of effectiveness humans. We therefore determined the expression level 21 abundant miRNAs to determine whether variation these was able explain RT-induced gains mass. Vastus lateralis biopsies were obtained from top and bottom ∼20% responders 56 young men...
Transcription of metabolic genes is transiently induced during recovery from exercise in skeletal muscle humans. To determine whether pre‐exercise glycogen content influences the magnitude and/or duration this adaptive response, six male subjects performed one‐legged cycling to lower one leg and then, following day, completed 2.5 h low intensity two‐legged exercise. Nuclei mRNA were isolated biopsies obtained vastus lateralis control reduced (pre‐exercise = 609 ± 47 337 33 mmol kg −1 dry...
1 This study examined the effect of ingesting caffeine (6 mg kg−1) on muscle carbohydrate and fat metabolism during steady-state exercise in humans. Young male subjects (n= 10) performed h (70 % maximal oxygen consumption (V̇O2,max)) two occasions (after ingestion placebo caffeine) leg was quantified by combination direct Fick measures biopsies. 2 Following serum fatty acid glycerol concentration increased (P≤ 0.05) at rest, suggesting enhanced adipose tissue lipolysis. 3 In addition...
In this study, the hypothesis that release of interleukin (IL)‐6 from human muscle is linked to exercise intensity and glucose uptake was investigated. overnight fasted state, seven healthy males performed knee extension exercise, kicking with both legs, each at 25 % maximal power ( W max ) for 45 min (eliciting 23 ± 1 pulmonary oxygen uptake, V̇ O2,max then simultaneously one leg 65 other 85 35 (40 ). Blood sampled a femoral artery veins, blood flow determined by thermodilution. Thigh...
1. Ten untrained young men ingested a carbohydrate‐rich diet (65 energy percent (E%) carbohydrate, T‐CHO) and ten similar subjects fat‐rich (62 E% fat, T‐FAT) while endurance training was performed 3‐4 times week for 7 weeks. For another 8th of both groups the (T‐CHO T‐FAT/CHO). 2. Maximal oxygen uptake increased by 11% (P < 0.05) in after 8 Time to exhaustion at 81% pre‐training maximal significantly from mean (+/‐ S.E.M.) 35 +/‐ 4 min 102 5 65 T‐CHO T‐FAT, respectively, weeks 0.05, vs....
To determine central and peripheral hemodynamic responses to upright leg cycling exercise, nine physically active men underwent measurements of arterial blood pressure gases, as well femoral subclavian vein flows gases during incremental exercise exhaustion (Wmax). Cardiac output (CO) flow (BF) increased in parallel with intensity. In contrast, arm BF remained at 0.8 l/min submaximal increasing 1.2 ± 0.2 maximal ( P < 0.05) when O 2 extraction reached 73 3%. The received a greater...
Interleukin (IL)-18 is associated with obesity, insulin resistance, and cardiovascular disease. The present study compared 1) IL-18 in adipocytes versus stromal vascular (SV) cells, 2) plasma adipose tissue (AT) obese lean subjects, 3) plasma, AT, skeletal muscle (SM) subjects after weight loss.At baseline, AT 23 were that 12 subjects. submitted to a 15-week life-style intervention (hypocaloric diet daily exercise) which samples, SM biopsies obtained. Analyses performed by ELISA RT-PCR...
Evidence suggests that sitting time is adversely associated with health risks. However, previous epidemiological studies have mainly addressed mortality whereas little known of the risk coronary heart disease. This study aimed to investigate total and myocardial infarction, disease incidence all-cause mortality.In Danish Health Examination Survey (DANHES) conducted in 2007-2008 we tested hypothesis a higher amount daily greater mortality. The population consisted 71,363 men women aged 18-99...
The mechanisms by which exercise benefits human health remain incompletely understood. With the emergence of omics techniques, mapping molecular response to is increasingly accessible. Here, we perform an untargeted metabolomics profiling plasma from a randomized, within-subjects, crossover study either endurance or resistance exercise, two types skeletal muscle activity that have differential effects on physiology. A high-resolution time-series analyses reveal shared as well...
High‐intensity interval training ( HIT ) is known to increase mitochondrial content in a similar way as endurance [60–90% of maximal oxygen uptake VO 2peak )]. Whether increases the mitochondria's ability oxidize lipids currently debated. We investigated effect on fat oxidation skeletal muscle and adipose tissue. Mitochondrial oxidative phosphorylation OXPHOS capacity, substrate sensitivity K m app ), were measured tissue healthy overweight subjects before after 6 weeks (three times per week...
Reference proteins (RP) or the total protein (TP) loaded is used to correct for uneven loading and/or transfer in Western blotting. However, signal sensitivity and influence of physiological conditions may question normalization methods. Therefore, three widely reference [β-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), α-tubulin], as well TP measured by Stain-Free technology (SF) tool were tested. This was done using skeletal muscle samples from men subjected often investigated...
Abstract Growing evidence supports that pharmacological application of growth differentiation factor 15 (GDF15) suppresses appetite but also promotes sickness-like behaviors in rodents via GDNF family receptor α-like (GFRAL)-dependent mechanisms. Conversely, the endogenous regulation GDF15 and its physiological effects on energy homeostasis behavior remain elusive. Here we show, four independent human studies prolonged endurance exercise increases circulating to levels otherwise only...
Mitochondria undergo continuous changes in shape as result of complex fusion and fission processes. The physiological relevance mitochondrial dynamics is still unclear. In the field mitochondria bioenergetics, there a need tools to assess cell content. To develop method visualize networks high resolution volume.Confocal fluorescence microscopy imaging network stains human vastus lateralis single muscle fibres focused ion beam/ scanning electron (FIB/SEM) imaging, combined with 3D...
Exercise has profound pleiotropic health benefits, yet the underlying mechanisms remain incompletely understood. Endocrine FGF21, bile acids (BAs), and BA-induced FGF19 have emerged as metabolic signaling molecules. Here, we investigated if dissimilar modes of exercise, resistance exercise (RE) endurance (EE), regulate plasma BAs, FGF19, FGF21 in humans.Ten healthy, moderately trained males were enrolled a randomized crossover study 1 hour bicycling at 70% VO2peak (EE) high-volume RE....
Little is known about the combined associations of cardiorespiratory fitness (CRF) and hand grip strength (GS) with mortality in general adult populations. The purpose this study was to compare relative risk for CRF, GS, their combination. In UK Biobank, a prospective cohort > 0.5 million adults aged 40–69 years, CRF measured through submaximal bike tests; GS using hand-dynamometer. This analysis based on data from 70,913 men women (832 all-cause, 177 cardiovascular 503 cancer deaths over...
Abstract Aim Metabolic health may deteriorate with age as a result of altered body composition and decreased physical activity. Endurance exercise is known to counter these changes delaying or even preventing onset metabolic diseases. High‐intensity interval training ( HIIT ) time efficient alternative regular endurance exercise, the aim this study was investigate benefit in older subjects. Methods Twenty‐two sedentary male (n = 11) female subjects aged 63 ± 1 years performed three...
Aging decreases skeletal muscle mass and strength, but aerobic resistance exercise training maintains function. NAD+ is a coenzyme for ATP production required substrate enzymes regulating cellular homeostasis. In muscle, mainly generated by the salvage pathway in which nicotinamide phosphoribosyltransferase (NAMPT) rate-limiting. NAMPT with age human increases levels young men. However, whether distinct modes of increase both old people unknown. We assessed effects 12 weeks on abundance...
1. This study was carried out to test the hypothesis that greater fat oxidation observed during exercise after adaptation a high-fat diet is due an increased uptake of originating from bloodstream. 2. Of 13 male untrained subjects, seven consumed fat-rich (62 % fat, 21 carbohydrate) and six carbohydrate-rich (20 65 carbohydrate). After 7 weeks training diet, 60 min bicycle performed at 68 +/- 1 maximum oxygen uptake. During [1-(13)C]palmitate infused, arterial venous femoral blood samples...
Training improves insulin sensitivity, which in turn may affect performance by modulation of fuel availability. Insulin action, turn, has been linked to specific patterns muscle structural lipids skeletal muscle. This study investigated whether regular exercise training exerts an effect on the membrane phospholipid fatty acid composition humans. Seven male subjects performed endurance knee extensors one leg for 4 wk. The other served as a control. Before, after days, and wk, biopsies were...