Magnesium oxide nanoparticles (MgO NPs) are increasingly recognized for their applications in cancer therapy such as nano-cryosurgery and hyperthermia. The present study investigated the cytotoxic effects of magnesium against normal lung fibroblast cells different types cancerous cells. MgO NPs exhibited a preferential ability to kill HeLa, AGS SNU-16 A detailed has been undertaken investigate mechanism cell death occurring (AGS cells) by analysis morphological changes, western blot flow...

Bananas, one of the most widely consumed fruits worldwide, are a rich source valuable phytochemicals. In this study, antioxidant and anticancer potential banana flesh was investigated. Of four kinds extracts, hexane extract (HE) had highest total polyphenol content (2.54 ± 0.60 mg GAE/g) flavonoid (1.69 0.34 RE/g), followed by chloroform fraction, ethanol extract, fraction. HE found to exert strong radical scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH•)...

Cysteine-rich angiogenic inducer 61 (CYR61) is an extracellular matrix-associated protein involved in survival, tumorigenesis, and drug resistance. Therefore, we examined the effects of flavones against CYR61-overexpressing human gastric adenocarcinoma AGS (AGS-cyr61) cells, which show remarkable resistance to 5-fluorouracil (5-FU), adriamycin (ADR), tamoxifen (TAM), paclitaxel (PAC), docetaxel (DOC). Among tested flavones, quercetin had lowest 50% inhibitory concentration (IC50)...

Callus suspension techniques have been considered attractive for improving bioactive metabolite productivity; methyl jasmonate (MeJA) is a widely used elicitor stimulating synthetic pathways. In this study, multivariate analysis-based metabolomics approach was employed to investigate the primary and specialized metabolites in leaves, unelicited calli, 100 or 200 μM MeJA elicited calli of

Magnolia flower buds are a source of herbal medicines with various active compounds. In this study, differences in the distribution and abundance major essential oils, phenolic acids, primary metabolites between white heptapeta violet denudata var. purpurascens were characterised. A multivariate analysis revealed clear separation respect to secondary closely related metabolic systems. White contained large amounts monoterpene hydrocarbons (MH), aromatic amino monosaccharides, production...

Abstract Constituents of supercritical CO2 extracts peel and flesh from pummelo fruits (Citrus grandis Osbeck) were analyzed by gas chromatography-mass spectrometry (GC-MS). In total, 69 constituents identified while 42 common in both samples. The major are 7-Methoxy-8- (2-oxo-3-methylbutyl) coumarin (11.4 %), (6E,8E,10E)-2,6,11,15-tetramethyl-2,6,8,10,14-hexadecapentaene (5.9 %) γ-sitosterol (5.1 hexadecanoic acid (10.0 (E,E)-2,4-decadienal (6.3 pentacosane (7.0 extracts. Antiproliferative...

Green mandarins are widely consumed unripe as mandarin oranges (Citrus unshiu Marcov.), which exhibit anti-inflammatory and anti-wrinkle effects by inhibiting the production of inflammatory cytokines matrix metalloproteinase. A randomized, double-blind, placebo-controlled clinical study was performed to verify skin improvement efficacy safety green extract (PTE). For standardization PTE, narirutin set a marker compound, PTE with constant content prepared for study. After randomizing subjects...

Current research highlights the use of natural products or phytochemicals as drugs and functional additives to treat obesity with few side effects. Sargassum horneri (SH) Ulva australis (UA) are marine waste resources on Jeju Island, Republic Korea. In this study, we analyzed their antioxidant anti-obesity efficacies confirm potential additives. We prepared SH UA extracts using 80% ethanol observed that free radical scavenging activity total phenol content were high in extracts, flavonoid...

제주도에서 생산된 로즈마리(Rosmarinus officinalis L.)와 라벤더(Lavandula angustifolia Mill.) 두 종의 허브 에센셜 오일과 하이드로졸 성분을 GC-MS로 분석하였다. 로즈마리 오일에는 <TEX>${\alpha}$</TEX>-pinene (40.96%)의 함량이 가장 높았으며, 라벤더 camphor (34.44%)가 높았다. 하이드로졸에는 verbenone이 (45.31%) 높게 나타났으며, 하이드로졸에서는 (67.04%)로 나타났다. 또한 로즈마리와 하이드로졸의 항산화 효능을 검증해 본 결과, 종류의 오일 모두 DPPH, ABTS 라디칼 소거능과, <TEX>$Fe^{2+}$</TEX> ion 킬레이팅 활성을 나타냈으나, alkyl 소거능은 거의 나타나지 않았다. DPPH 오일이 오일보다 우수한 반면, 실험에서는 더 우수했다. 로즈마리, 소거능을 하이드로졸만이 나타냈다. 특히 <TEX>$H_2O_2$</TEX>에 의해 유도된...

Activation of signal transducer and activator transcription 3 (STAT3) is well known to play a major role in the cell growth, survival, proliferation, metastasis, angiogenesis various cancer cells. Most citrus species offer large quantities phytochemicals that have beneficial effects attributed their chemical components. Our study was carried out evaluate anticancer pericarp Iyokan ( Citrus iyo Hort. ex Tanaka), locally as yeagam Korea, through modulation STAT3 signaling pathway both tumor...

Supercritical CO2 extract from fruits of Citrus hassaku Hort ex Tanaka, iyo Hort. and grandis Osbeck, locally known as phalsak, yeagam, dangyuja, respectively, in Korea, were analyzed by gas chromatography-mass spectrometry (GC-MS). In total, 70, 79, 76 compounds identified dangyuja respectively. (Z)-9-Octadecenoic acid was the major compound whereas limonene prevailed among others. α-Terpineol, (E,E)-2,4-decadienal, hexadecanoic acid, pentacosane, stigmasterol, γ-sitosterol prominent all...

Compositions of essential oils extracted from mint herb such as Mentha piperita, spicata, and × piperita var. citrate produced in Jeju were analyzed using gas chromatography-mass spectrometry (GC-MS) headspace-GC-MS (HS-GC-MS). By the GC-MS analysis, 13 compounds tentatively identified citrate, respectively. Peperitenone oxide, carvone, linalool detected major respectively, based on ratio peak intensity total ion chromatogram. The greater number compounds, including volatile alcohols...

In Jeju-native Citrus, flavonoids are the main contributors to various types of biological activity, such as antioxidant, antitumor, and anti-inflammatory activity. Thus, we developed simultaneous quantification methods for analysis ten bioactive in Jeju Citrus fruits (Dangyuja, Gamja, Jigak, Sadugam, Soyuja) harvested at six different time points using a high-performance liquid chromatography–diode array detector (HPLC-DAD). Separation was performed flow rate 0.8 mL/min, column temperature...

Cynanchum wilfordii root is used in traditional herbal medicine owing to its various pharmacological activities. However, C. roots are misused their morphological similarities with auriculatum. Adventitious (AR) culture can prevent such misuse, and the selection of plant materials an important procedure for producing high-quality ARs. This study aimed compare proliferation metabolic profiles ARs two types explants from different cultivation methods (either cultivated open field (ECF) or on a...

This study investigated the safety and functionality of a functional additive for humans animals from Sargassum horneri (SH) Ulva australis (UA) waste recycling marine refuse generated in large quantities Jeju. Sprague-Dawley rats were orally administered additives at 2,000 mg/kg to assess 14-day repeated dose toxicity two extracts. For female rats, weight gain after administration SH was 66.2±18.8% vs. controls. Male UA showed 92.3±8.0% significantly decreased serum glucose levels male...

본 연구의 목적은 제주도 재래감귤인 팔삭과 이예감 초임계 추출물에 대한 인간 자궁암 세포인 HeLa 세포에서의 성장억제 효능을 탐색하고, 각 추출물에서의 유효 활성 성분을 분석하는데 있다. 및 세포사멸 탐색하기 위해 MTT assay와 Hoechst 33342 염색을 수행하였으며, 성분분석은 가스크로마토그래피 질량분석기(GC/MS)를 이용하였다. 두 종류의 미성숙 감귤 과육, 과피 세포의 성장 억제 비교해 결과, 추출물은 모두 비슷한 나타냈다. 추출물을 처리한 세포에서는 농도 의존적으로 성장률이 억제되었으며, 처리 <TEX>$400{\mu}g/mL$</TEX>에서 추출물이 각각 87.16%와 92.95%의 나타냈으나, 과육 추출물의 세포 효능은 <TEX>$400{\mu}g/mL$</TEX>까지 관찰되지 않았다. 통해 apoptotic body 형성을 현미경으로 관찰한 100, <TEX>$200{\mu}g/mL$</TEX> apoptpotic body를 관찰할 수 없었으나,...

Little information about the biological activities of Citrus limon (lemon) leaves has been reported, whereas fruit well-documented to contain various pro-health bio-functional compounds. In present study, antiproliferative lemon were evaluated using several cancer cell lines. From n-hexane, chloroform, ethyl acetate, n-butanol, and water fractions methanolic extract leaves, chloroform fraction (CFLL) showed most potent activity in AGS human gastric cells. The current study demonstrates that...

In this study, the safety and functionality of using citrus juice processing waste (CJPW) was confirmed. Large quantities CJPW are generated on Jeju Island cause environmental problems. extract (2,000 mg/kg) administered intragastrically to male female Sprague-Dawley (SD) rats for 14 days were analyzed. No general signs toxicity observed in SD extract. Feed intake did not differ between experimental control animals. However, had greater weight gain (102.9±5.53% 114.15±6.89%, respectively)...

The anticancer activity of a methanolic extract from lemon leaves (MLL) was assessed in MCF-7-SC human breast cancer stem cells. MLL induced apoptosis MCF-7-SC, as evidenced by increased apoptotic body formation, sub-G1 cell population, annexin V-positive cells, Bax/Bcl-2 ratio, well proteolytic activation caspase-9 and caspase-3, degradation poly (ADP-ribose) polymerase (PARP) protein. Concomitantly, the formation acidic vesicular organelles, LC3-II accumulation, reduced Akt, mTOR, p70S6K,...

본 연구는 삼채의 조추출물과 유기용매 분획물들을 가지고 DPPH 라디칼 소거 활성에 의한 항산화 활성 검색 결과 디클로로메탄(CHSUB2/SUBClSUB2/SUB) 분획물과 에틸아세테이트(EtOAc) 분획물에서 활성을 나타냈으며, xanthine oxidase 억제 효과는 활성에서 제일 뛰어났던 에틸아세테이트 분획물에서, superoxide 활성은 헥산(n-hexane) 활성이 나타났다. RAW 264.7 세포에 lipopolysaccharide로 자극을 주고 삼채 주정 추출물 및 처리하여 확인해본 결과, 물 분획물을 제외한 나머지 염증 유발 인자(NO, PGESUB2/SUB, iNOS, COX-2, IL-6 IL-1β) 생성억제 효과가 나타났으며, 그중 디클로로메탄 효과를 확인할 수 있었다. 실험 효과 인자의 생성 이러한 삼채에서 유효성분 추출을 통한 항산화, 항염증 물질의 연구 또는 예방하거나 치료할 있는 성분의 분리 그 작용기전 연구에 중요한 기초 자료가 될 것이라 생각한다.