A5073270407- Urinary Tract Infections Management
- Gut microbiota and health
- Pelvic floor disorders treatments
- Bladder and Urothelial Cancer Treatments
- Urinary Bladder and Prostate Research
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Dermatology and Skin Diseases
- Microbial Metabolism and Applications
- Estrogen and related hormone effects
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Biochemical and Structural Characterization
- Reproductive tract infections research
- Sexual function and dysfunction studies
The University of Texas at Dallas
2020-2023
Postmenopausal women are severely affected by recurrent urinary tract infection (rUTI). The urogenital microbiome is a key component of the environment. However, changes in underlying rUTI susceptibility unknown. Here, we perform shotgun metagenomics and advanced culture on urine from controlled cohort postmenopausal to identify compositional function linked susceptibility. We candidate taxonomic biomarkers an enrichment lactobacilli taking estrogen hormone therapy. find robust correlations...
Urinary tract infection (UTI) is a global health issue that imposes substantial burden on healthcare systems. Women are disproportionately affected by UTI, with >60% of women experiencing at least one UTI in their lifetime. UTIs can recur, particularly postmenopausal women, leading to diminished quality life and potentially life-threatening complications. Understanding how pathogens colonize survive the urinary necessary identify new therapeutic targets urgently needed due rising rates...
Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units uronic acid and amino sugars. The luminal surface the bladder epithelium is coated with a GAG layer. These urothelial GAGs thought to provide protective barrier serve as potential interaction site urinary microbiome (urobiome). Previous studies have profiled composition in mixed cohorts, but postmenopausal women remains undefined. To investigate relationship between recurrent...
Enterococcus raffinosus is an understudied member of its genus possessing a characteristic megaplasmid contributing to large genome size. Although less commonly associated with human infection compared other enterococci, this species can cause disease and persist in diverse niches such as gut, urinary tract, blood, environment. Few complete assemblies have been published date for E. raffinosus. In study, we report the assembly first clinical strain, Er676, isolated from postmenopausal woman...
Glycosaminoglycans (GAGs) are linear polysaccharides and among the primary components of mucosal surfaces in mammalian systems. The GAG layer lining surface urinary tract is thought to play a critical role homeostasis provide barrier against infection (UTI). This key component host-microbe interface may serve as scaffolding site or nutrient source for microbiota invading pathogens, but its exact UTI pathogenesis unclear. Although members gut have been shown degrade GAGs, utilization...
Complete genome sequences provide valuable data for the understanding of genetic diversity and unique colonization factors urinary microbes. These may include mobile elements, such as plasmids extrachromosomal phage, that contribute to dissemination antimicrobial resistance further complicate treatment tract infection (UTI). In addition providing fine resolution structure, complete, closed genomes allow detailed comparative genomics evolutionary analyses. The generation complete de novo has...
is an understudied member of its genus possessing a characteristic megaplasmid contributing to large genome size. Although less commonly associated with human infection compared other enterococci, this species can cause disease and persist in diverse niches such as the gut, urinary tract, blood environment. Few complete assemblies have been published date for
Lactobacillus crispatus frequently colonizes the vagina and bladder of healthy women. Although its association with vaginal health is relatively well understood, little known about role in urinary tract infection (UTI). Here, we report complete genome sequences three L. strains isolated from women different UTI histories.
Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infection (UTI). This disease disproportionately affects women and frequently develops into recurrent UTI (rUTI) in postmenopausal women. Here, we report complete genome sequences seven UPEC isolates obtained from urine with rUTI.
Abstract Community-acquired urinary tract infection (UTI) is among the most common bacterial infections observed in humans. Postmenopausal women are a rapidly growing and underserved demographic group who severely affected by recurrent UTI (rUTI) with >50% recurrence rate. In this population, rUTI can persist for years, reducing quality of life imposing significant healthcare burden. often treated antibiotics, but development antibiotic resistance allergy limit therapeutic options. The...
You have accessJournal of UrologyInfections/Inflammation/Cystic Disease the Genitourinary Tract: Kidney & Bladder I (MP69)1 May 2024MP69-02 THE IMPACT OF RECURRENT URINARY TRACT INFECTION AND UROBIOME ECOLOGY ON METABOLOME Michael L. Neugent, Neha V. Hulyalkar, Kevin C. Lutz, Qiwei Li, Philippe E. Zimmern, Vladimir Shulaev, and Nicole J. De Nisco NeugentMichael Neugent , HulyalkarNeha Hulyalkar LutzKevin Lutz LiQiwei Li ZimmernPhilippe Zimmern ShulaevVladimir Shulaev NiscoNicole View All...
Abstract Recurrent urinary tract infections (rUTIs) are a major clinical challenge in postmenopausal women and their increasing prevalence underscores the need to define interactions between host microbiome that may underlie rUTI susceptibility. A body of work has identified taxonomic profile female associate with aging, menopause, urinay disease. However, how this microbial community engages niche, including local biochemical environment urogenital tract, health disease is yet be fully...
Enterococcus faecalis is the leading Gram-positive bacterial species implicated in urinary tract infection (UTI). An opportunistic pathogen, E. a commensal of human gastrointestinal (GIT) and its presence GIT predisposing factor for UTI. The mechanisms by which colonizes survives (UT) are poorly understood, especially uncomplicated or recurrent UT distinct from characterized sparse nutrient landscape unique environmental stressors. In this study, we isolated sequenced collection 37 clinical...
Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units uronic acid and amino sugars. The luminal surface the bladder epithelium is coated with a GAG layer. These urothelial GAGs thought to provide protective barrier serve as potential interaction site urinary microbiome (urobiome). Previous studies have profiled composition in mixed cohorts, but postmenopausal women remains undefined. To investigate relationship between recurrent UTI...
You have accessJournal of UrologyCME1 Apr 2023MP34-11 URINARY GLYCOSAMINOGLYCANS ARE ASSOCIATED WITH RECURRENT UTI AND UROBIOME ECOLOGY IN POSTMENOPAUSAL WOMEN Michael L. Neugent, Neha V. Hulyalkar, Philippe E. Zimmern, Kelli Palmer, Vladimir Shulaev, and Nicole J. De Nisco NeugentMichael Neugent More articles by this author , HulyalkarNeha Hulyalkar ZimmernPhilippe Zimmern PalmerKelli Palmer ShulaevVladimir Shulaev NiscoNicole View All Author...
You have accessJournal of UrologyInfections/Inflammation/Cystic Disease the Genitourinary Tract: Kidney & Bladder I (MP46)1 Apr 2020MP46-02 METAGENOMIC ANALYSIS OF THE GENITOURINARY MICROBIOME POSTMENOPAUSAL WOMEN WITH RECURRENT UTI Michael Neugent, Neha Hulyalkar, Ashwani Kumar, Vivian Nguyen, Amy Kuprasertkul, Jorge Fuentes, Chao Xing, Philippe Zimmern, Kelli Palmer, and Nicole De Nisco* NeugentMichael Neugent More articles by this author , HulyalkarNeha Hulyalkar KumarAshwani Kumar...
You have accessJournal of UrologyCME1 May 2022MP38-05 TARGETED LIQUID CHROMATOGRAPHY MASS SPECTROMETRY QUANTIFICATION OF EXCRETED URINARY ESTROGENS AMONG POSTMENOPAUSAL WOMEN USING DIFFERENT ESTROGEN HORMONE THERAPY MODALITIES Michael L. Neugent, Neha Hulyalkar, Vladimir Shulaev, Philippe E. Zimmern, and Nicole J. De Nisco NeugentMichael Neugent More articles by this author , HulyalkarNeha Hulyalkar ShulaevVladimir Shulaev ZimmernPhilippe Zimmern NiscoNicole View All Author...
Enterococcus raffinosus is an understudied member of its genus possessing a characteristic megaplasmid contributing to significantly large genome size. Although less commonly associated with human infection compared other enterococci, this species can cause disease and persist in diverse niches such as gut, urinary tract, blood, environment. Few complete assemblies have been published date for E. raffinosus. In study, we report the assembly first clinical strain, Er676, isolated from...
Urinary tract infections (UTIs) are among the most common adult bacterial infections. When a patient suffers ≥3 UTIs per year, it is defined as recurrent UTI (rUTI). Current rUTI therapies rely on antimicrobials to achieve sterility in urinary (UT). However, research has shown that urine not sterile healthy individuals and microbiota been fully characterized. A constant flux of containing electrolytes, osmolytes, amino acids, carbohydrates supply nutrients support microbiota. An overlooked...
Complete genome sequences provide valuable data for the understanding of genetic diversity and unique colonization factors urinary microbes. These may include mobile elements, such as plasmids extrachromosomal phage, that contribute to dissemination antimicrobial resistance further complicate treatment tract infection (UTI). In addition providing fine resolution structure, complete, closed genomes allow detailed comparative genomics evolutionary analyses. The generation complete de novo has...