A5006858052- Urinary Tract Infections Management
- Gut microbiota and health
- Pelvic floor disorders treatments
- Urinary Bladder and Prostate Research
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Bayesian Methods and Mixture Models
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Metabolism, Diabetes, and Cancer
- Dermatology and Skin Diseases
- Zoonotic diseases and public health
- Atherosclerosis and Cardiovascular Diseases
- Neonatal Respiratory Health Research
- Machine Learning in Healthcare
- Enterobacteriaceae and Cronobacter Research
- Sexual function and dysfunction studies
- Mental Health Research Topics
- Bioinformatics and Genomic Networks
- Medical Imaging and Pathology Studies
- Cancer Diagnosis and Treatment
- Metastasis and carcinoma case studies
- Cardiac Fibrosis and Remodeling
- Bacteriophages and microbial interactions
The University of Texas at Dallas
2017-2025
ABSTRACT Advances in next‐generation sequencing technology have enabled the high‐throughput profiling of metagenomes and accelerated microbiome studies. Recently, there has been a rise quantitative studies that aim to decipher co‐occurrence network its underlying community structure based on metagenomic sequence data. Uncovering complex is essential understanding role disease progression susceptibility. Taxonomic abundance data generated from technologies are high‐dimensional compositional,...
Abstract Adenocarcinoma (ADC) and squamous cell carcinoma (SqCC) are the two predominant subtypes of non-small lung cancer (NSCLC) distinct in their histological, molecular clinical presentation. However, metabolic signatures specific to individual NSCLC remain unknown. Here, we perform an integrative analysis human tumour samples, patient-derived xenografts, murine model NSCLC, lines The Cancer Genome Atlas (TCGA) reveal a markedly elevated expression GLUT1 glucose transporter SqCC, which...
Hypoxia has long been implicated in the pathogenesis of fibrotic diseases. Aberrantly activated myofibroblasts are primary pathological driver progression, yet how various microenvironmental influences, such as hypoxia, contribute to their sustained activation and differentiation is poorly understood. As a defining feature hypoxia its impact on cellular metabolism, we sought investigate hypoxia-induced metabolic reprogramming affects myofibroblast test preclinical efficacy targeting...
Abstract The fibrogenic response in tissue-resident fibroblasts is determined by the balance between activation and repression signals from tissue microenvironment. While molecular pathways which transforming growth factor-1 (TGF-β1) activates pro-fibrogenic mechanisms have been extensively studied are recognized critical during fibrosis development, factors regulating TGF-β1 signaling poorly understood. Here we show that macrophage hypoxia suppresses excessive a heart via oncostatin-m (OSM)...
Postmenopausal women are severely affected by recurrent urinary tract infection (rUTI). The urogenital microbiome is a key component of the environment. However, changes in underlying rUTI susceptibility unknown. Here, we perform shotgun metagenomics and advanced culture on urine from controlled cohort postmenopausal to identify compositional function linked susceptibility. We candidate taxonomic biomarkers an enrichment lactobacilli taking estrogen hormone therapy. find robust correlations...
Urinary tract infection (UTI) is a global health issue that imposes substantial burden on healthcare systems. Women are disproportionately affected by UTI, with >60% of women experiencing at least one UTI in their lifetime. UTIs can recur, particularly postmenopausal women, leading to diminished quality life and potentially life-threatening complications. Understanding how pathogens colonize survive the urinary necessary identify new therapeutic targets urgently needed due rising rates...
Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units uronic acid and amino sugars. The luminal surface the bladder epithelium is coated with a GAG layer. These urothelial GAGs thought to provide protective barrier serve as potential interaction site urinary microbiome (urobiome). Previous studies have profiled composition in mixed cohorts, but postmenopausal women remains undefined. To investigate relationship between recurrent...
In the last decade, numerous statistical methods have been developed for analyzing microbiome data generated from high-throughput next-generation sequencing technology. Microbiome are typically characterized by zero inflation, overdispersion, high dimensionality, and sample heterogeneity. Three popular areas of interest in research requiring that can account characterizations include detecting differentially abundant taxa across phenotype groups, identifying associations between covariates,...
Glycosaminoglycans (GAGs) are linear polysaccharides and among the primary components of mucosal surfaces in mammalian systems. The GAG layer lining surface urinary tract is thought to play a critical role homeostasis provide barrier against infection (UTI). This key component host-microbe interface may serve as scaffolding site or nutrient source for microbiota invading pathogens, but its exact UTI pathogenesis unclear. Although members gut have been shown degrade GAGs, utilization...
Urinary tract infection (UTI) is one of the most common adult bacterial infections and exhibits high recurrence rates, especially in postmenopausal women. Studies mouse models suggest that cyclooxygenase-2 (COX-2)–mediated inflammation sensitizes bladder to recurrent UTI (rUTI). However, COX-2–mediated has not been robustly studied human rUTI. We used cohorts assess urothelial COX-2 production evaluate its product, PGE 2 , as a biomarker for rUTI found percentage COX-2–positive cells was...
Cancer cells consume high amounts of glucose for their cellular bioenergetic and anabolic requirements, relying on to fuel growth. We recently reported that lung squamous cell carcinoma, a major subtype non-small cancer (NSCLC), exhibits remarkably elevated transporter GLUT1 (encoded by SLC2A1) expression dependency, while another NSCLC, adenocarcinoma, shows significant independence. Our findings highlight the metabolic heterogeneity metabolism among subtypes, which can be exploited...
Visualization of the interaction bacteria with host mucosal surfaces and tissues can provide valuable insight into mechanisms pathogenesis. While visualization bacterial pathogens in animal models infection rely on strains engineered to express fluorescent proteins such as GFP, within mucosa biopsies or tissue obtained from human patients requires an unbiased method. Here, we describe efficient method for detection tissue-associated biopsy sections. This utilizes situ hybridization (FISH) a...
You have accessJournal of UrologyInfections/Inflammation/Cystic Disease the Genitourinary Tract: Kidney & Bladder I (MP69)1 May 2024MP69-02 THE IMPACT OF RECURRENT URINARY TRACT INFECTION AND UROBIOME ECOLOGY ON METABOLOME Michael L. Neugent, Neha V. Hulyalkar, Kevin C. Lutz, Qiwei Li, Philippe E. Zimmern, Vladimir Shulaev, and Nicole J. De Nisco NeugentMichael Neugent , HulyalkarNeha Hulyalkar LutzKevin Lutz LiQiwei Li ZimmernPhilippe Zimmern ShulaevVladimir Shulaev NiscoNicole View All...
The human body consists of microbiomes associated with the development and prevention several diseases. These microbial organisms form complex interactions that are informative to scientific community for explaining disease progression prevention. Contrary traditional view microbiome as a singular, assortative network, we introduce novel statistical approach using weighted stochastic infinite block model analyze structures within co-occurrence interaction networks. Our defines connections...
Abstract Community-acquired urinary tract infection (UTI) is among the most common bacterial infections observed in humans. Postmenopausal women are a rapidly growing and underserved demographic group who severely affected by recurrent UTI (rUTI) with >50% recurrence rate. In this population, rUTI can persist for years, reducing quality of life imposing significant healthcare burden. often treated antibiotics, but development antibiotic resistance allergy limit therapeutic options. The...
You have accessJournal of UrologyInfections/Inflammation/Cystic Disease the Genitourinary Tract: Kidney & Bladder I (MP69)1 May 2024MP69-15 METAGENOMIC SEQUENCING OF THE URINARY MICROBIOME POSTMENOPAUSAL WOMEN WITH RECURRENT TRACT INFECTION AND TYPE 2 DIABETES Sara B. Papp, Michael L. Neugent, Belle M. Sharon, Philippe E. Zimmern, and Nicole J. De Nisco PappSara Papp , NeugentMichael Neugent SharonBelle Sharon ZimmernPhilippe Zimmern NiscoNicole View All Author...