A5015883250- Tendon Structure and Treatment
- Protease and Inhibitor Mechanisms
- Shoulder Injury and Treatment
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Sports injuries and prevention
- Osteoarthritis Treatment and Mechanisms
- Exercise and Physiological Responses
- Immunotherapy and Immune Responses
- Carbohydrate Chemistry and Synthesis
- Inflammatory mediators and NSAID effects
- Natural product bioactivities and synthesis
- Angiogenesis and VEGF in Cancer
- Diabetes Treatment and Management
- Connective tissue disorders research
- Organic Chemistry Cycloaddition Reactions
- Orthopedic Surgery and Rehabilitation
- Peptidase Inhibition and Analysis
- Blood Coagulation and Thrombosis Mechanisms
- Veterinary Equine Medical Research
- Kruppel-like factors research
- Orthopaedic implants and arthroplasty
BioPharm (United Kingdom)
2016
GlaxoSmithKline (United Kingdom)
2016
University of East Anglia
2008-2013
Norwich Research Park
2013
Biopharma Technology (United Kingdom)
2013
Addenbrooke's Hospital
2004-2008
University of Sheffield
2003-2004
Sheffield Children's Hospital
2004
Leiden University
2002
European Society for Blood and Marrow Transplantation
2001
Abstract Objective To profile the messenger RNA (mRNA) expression for 23 known genes of matrix metalloproteinases (MMPs), 19 ADAMTS, 4 tissue inhibitors (TIMPs), and ADAM 8, 10, 12, 17 in normal, painful, ruptured Achilles tendons. Methods Tendon samples were obtained from cadavers or patients undergoing surgical procedures to treat chronic painful tendinopathy tendon. Total was extracted mRNA analyzed by quantitative real‐time reverse transcription–polymerase chain reaction, normalized 18S...
Repetitive strain or ‘overuse’ is thought to be a major factor contributing the development of tendinopathy. The aims our study were develop novel cyclic loading system, and use it investigate effect defined conditions on mechanical properties gene expression isolated tendon fascicles. Tendon fascicles dissected from bovine‐foot extensors subjected tensile (1 Hz ) at 30% 60% failure, for 0 h (control), 15 min, 30 1 h, 5 h. Post loading, quasi‐static test failure assessed damage. Gene...
Tendinopathies are a range of diseases characterised by degeneration and chronic tendon pain represent significant cause morbidity. Relatively little is known about the underlying mechanisms; however onset often associated with physical activity. A number molecular changes have been documented in tendinopathy such as decrease overall collagen content, increased extracellular matrix turnover protease Metalloproteinases involved homeostasis expression regulated mechanical strain. The aims this...
Three mammalian ADAMTS enzymes, ADAMTS‐1, ‐4 and ‐5, are known to cleave aggrecan at certain glutamyl bonds considered be largely responsible for cartilage catabolism observed during the development of arthritis. We have previously reported that catechins, polyphenolic compounds found in highest concentration green tea ( Camellia sinensis ), capable inhibiting breakdown an vitro model degradation. now cloned expressed recombinant human ‐5 report here catechin gallate esters potently inhibit...
Several members of the ADAMTS (A Disintegrin And Metalloproteinase with ThromboSpondin motifs) family have been identified as aggrecanases, whose substrates include versican, principal large proteoglycan in tendon extracellular matrix. We characterized expression ADAMTS-4 human Achilles and tendon-derived cells. mRNA levels were higher ruptured compared normal or chronic painful tendinopathy. In tissue extracts probed by Western blotting, mature (68 kDa) was detected only tendons, while...
Glucagon-like peptide-1 (GLP-1) and its mimetics reduce infarct size in the setting of acute myocardial ischemia/reperfusion (I/R) injury. However, short serum half-life GLP-1 may limit their therapeutic use ischemia. Domain antibodies to albumin (AlbudAbs) have been developed extend lived proteins, peptides small molecules. In this study, we compared effect a long acting agonist, DPP-IV resistant (7–36, A8G) fused an AlbudAb (GAlbudAb), with mimetic, exendin-4 (short agonist) on following...
Aberrant WNT signaling is associated with the formation and growth of numerous human cancer types. The low-density lipoprotein receptor-related protein 6 (LRP6) least redundant component receptor complex two independent ligand-binding sites. Using domain antibody (dAb) technology, a bispecific (GSK3178022) to LRP6 was identified that capable blocking stimulation in presence range R-spondin (RSPO) ligands vitro GSK3178022 also efficacious reducing target gene expression vivo, both cell line...
Our aim was to examine, using microsatellite (ms) markers, the contribution of telomeric part HLA region rheumatoid arthritis (RA) predisposition in Spanish population. We have looked at distribution DQB1, DRBI and five ms loci (D6S1014, D6S273, D6STNFa, MIB C1-2-5) within 147 RA patients 202 control subjects. A total 19 conserved configurations were observed, twelve them linkage disequilibrium with particular DQB1-DRB1 haplotypes. Interestingly, haplotype c1...
Introduction In combination with the catalytic domain, ancillary domains of ADAMTS' are proposed to regulate activity via interactions sulfated GAGs, extracellular matrix (ECM) and cell surface. Interactions both GAGs ECM have been attributed thrombospondin (TSP) type 1 motifs spacer region ( Kuno Matsushima 1998 ; Flannery et al . 2002 ). ADAMTS‐1, ‐4 ‐5, all undergo cleavage within their respective regions Rodriguez‐Manzaneque 2000 Georgiadis ), an event which has reported increase towards...
Abstract Die Kondensation der p‐Hydroxy‐benzaldehyde (I) mit Phenylessigsäuren (II) liefert die Stilben‐Derivate (III), NaH, Epichlorhydrin und Aminen in Aminophenolether (IV) übergeführt werden.