A5033042169- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Trypanosoma species research and implications
- Plant Pathogenic Bacteria Studies
- Research on Leishmaniasis Studies
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Calcium signaling and nucleotide metabolism
- Photosynthetic Processes and Mechanisms
- Soybean genetics and cultivation
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Mast cells and histamine
- Plant Gene Expression Analysis
- Nuclear Receptors and Signaling
- Protein purification and stability
- Antimicrobial Peptides and Activities
- Cardiac pacing and defibrillation studies
- Plant Stress Responses and Tolerance
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Toxoplasma gondii Research Studies
- Leaf Properties and Growth Measurement
University of Toronto
2014-2025
The Scarborough Hospital
2019-2024
Lonza (Switzerland)
2018
Centre for Immunity, Infection and Evolution
2011-2017
University of Edinburgh
2011-2017
Harvard University
2006-2011
The recognition of microbe-associated molecular patterns during infection is central to the mounting an effective immune response. In spite their importance, it remains difficult identify these molecules and host receptors required for perception, ultimately limiting our understanding role in evolution host-pathogen relationships. We employ a comparative genomics screen six new eliciting peptides from phytopathogenic bacterium Pseudomonas syringae. then perform reverse genetic Arabidopsis...
Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity Its ADP-ribosyltransferase activity. Proteomic analysis interacting proteins identified a protein complex containing ATPases required for regulating stomatal aperture, suggesting may manipulate immunity. Here we report inhibit independent its Transgenic expression in inhibits closing response to P. and increases the...
Plants are sessile organisms and must accurately respond to a host variety of growth, developmental, environmental signals throughout their life maximize fitness. Plant cell surface receptor-like kinases ideal for the perception such transduction within cell. The Strubbelig Receptor Family (SRF) is group leucine-rich repeat kinases, several which have unknown function. Here, we identify role SRF6 in wall damage activation downstream immune responses. We show that necessary proper responses...
Pseudomonas syringae employs a type III secretion system to inject 20–30 different effector (T3SE) proteins into plant host cells. A major role of T3SEs is suppress immune responses and promote bacterial infection. The YopJ/HopZ acetyltransferases are superfamily found in both animal pathogenic bacteria. In P. syringae, this includes the evolutionarily diverse HopZ1, HopZ2 HopZ3 alleles. To investigate roles HopZ family immunomodulation, we generated dexamethasone-inducible T3SE transgenic...
There are hundreds of Trypanosoma species that live in the blood and tissue spaces their vertebrate hosts. The vast majority these do not have ornate system antigenic variation has evolved small number African trypanosome species, but can still maintain long-term infections face adaptive immune system. theileri is a typical example, restricted host range cattle other Bovinae, only occasionally reported to cause patent disease although no systematic survey effect infection on agricultural...
Abstract To investigate the effects of Trypanosoma cruzi on mechanical properties infected host cells, cytoskeletal stiffness and remodeling dynamics were measured in parasite‐infected fibroblasts. We find that cell decreases a time‐dependent fashion T. ‐infected human foreskin fibroblasts without significant change remodeling. In contrast, cells exposed to secreted/released components become significantly stiffer within 2 h exposure exhibit increased dynamics. These findings represent first...
The first step in the plant immune response to pathogen challenge involves perception of conserved epitopes, called microbe-associated molecular patterns (MAMPs), by cell-surface pattern recognition receptors (PRRs). Given key roles that MAMPs and PRRs play innate immunity, great effort has been expended identify these molecules. Current methods for assaying responses are often limited their resolution throughput, consequently, there is a need medium- high-throughput methodologies. Here, we...
Abstract Plants use surface receptors to perceive information about many aspects of their local environment. These physically interact form both steady state and signalling competent complexes. The events downstream receptor activation impact plant developmental immune responses. Here, we present a comprehensive study the physical interactions between extracellular domains leucine-rich repeat kinases (LRR-RKs) in Arabidopsis. Using sensitized assay, tested reciprocal among 200 225...
To maximize overall fitness, plants must accurately respond to a host of growth, developmental, and environmental signals throughout their life. Many these internal external are perceived by the leucine-rich repeat receptor-like kinases, which play roles in regulating development, immunity. This largest family receptor kinases can be divided into subfamilies based on conservation kinase domain, demonstrates that shared evolutionary history often indicates molecular function. Here we...
The plant Arabidopsis thaliana is a model system used by researchers through much of research. Recent efforts have focused on discovering the genomic variation found in naturally occurring ecotypes isolated from around world. These come diverse climates and therefore faced adapted to variety abiotic biotic stressors. sequencing comparative analysis these genomes can offer insight into adaptive strategies plants. While there are large number ecotype genome sequences available, majority were...
Trypanosomatid parasites are notorious for the human diseases they cause throughout Africa and South America. However, non-pathogenic trypanosomatids also found worldwide, infecting a wide range of hosts. One example is Trypanosoma (Megatrypanum) theileri, ubiquitous protozoan commensal bovids, which distributed globally. Exploiting knowledge pathogenic trypanosomatids, we have developed theileri as novel vehicle to deliver vaccine antigens other proteins cattle. Conditions growth...
The protozoan parasite Trypanosoma cruzi, which causes human Chagas' disease, exerts a variety of effects on host extracellular matrix (ECM) including proteolytic degradation collagens and dampening ECM gene expression. Exposure primary dermal fibroblasts to live infective T. cruzi trypomastigotes or their shed/secreted products results in rapid down-regulation the fibrogenic genes collagenIα1, fibronectin connective tissue growth factor (CTGF/CCN2). Here we demonstrate ability...
Abstract Plants are exposed to a variety of growth, developmental, and environmental cues during their lifespan. To survive thrive, plants have developed sophisticated ways responding these signals that involve regulation at the transcriptional, post-transcriptional, translational, post-translational levels. Leucine-rich repeat receptor-like kinases largest family in respond range external internal stimuli. They act as crucial regulators plant development, immunity. fully understand LRR-RLK...
Among plants, there is considerable variation in lifespan: annuals live less than one year, whereas perennials for several years, with the longest-living perennial having survived 43,600 years. As proposed by Disposable Soma Theory, this lifespan among plants likely reflects differential investment of limited energy and nutrient resources, investing more nutrients into biomolecular maintenance compared to order ensure persistence over multiple seasons. Such may be particularly important...
Chromatography resins used for purifying biopharmaceuticals are generally dedicated to a single product. In good manufacturing practice (GMP) facilities that manufacture limited amount of any particular product, this can result in the resin being fraction its useful life. A methodology extending reuse multiple products is described. With methodology, and column performance, product carryover, cleaning effectiveness continually monitored ensure quality not affected by multiproduct (MRR)....
Abstract To maximize overall fitness, plants must accurately respond to a host of growth, developmental, and environmental signals throughout their life. Many these internal external are perceived by the leucine-rich repeat receptor-like kinases, which play roles in regulating development, immunity. This largest family receptor kinases can be divided into subfamilies based on conservation kinase domain, demonstrates that shared evolutionary history often indicates molecular function. Here we...
The initial stage of plant defence against pathogen challenge is dependent on the perception microbe-associated molecular patterns (MAMPs) by cognate pattern recognition receptors (PRRs), leading to a basal immune response referred as pattern-triggered immunity (PTI). This early and broad-based very active area research, in part because it represents an excellent route improving crop performance. As more MAMPs PRRs are identified, mainly model species Arabidopsis thaliana, there related...
Abstract Among plants, there is considerable variation in maximum lifespan, with annuals living less than one year and perennials up to 43 600 years. This lifespan may reflect differential investment of limited energy resources, investing more into biomolecular damage prevention and/or repair order ensure their persistence over multiple seasons. The present study evaluated this prediction using chlorophyll from annual perennial flax (Linum L.). We investigated the degradation...