Pathogenic bacteria cause disease outbreaks and threaten human health, prompting the research on advanced detection assays. Herein, we developed a selective molecular imprinted aptasensor for sensitive prompt quantitation of Staphylococcus aureus (S. aureus) bacteria. The was constructed by immobilization aptamer gold nanoparticles modified magnetic (apt-AuNPs@ Fe3O4). A functional monomer (o-phenylenediamine, o-phen) electro-polymerized surface as-synthesized nanocomposite in presence...

Autoimmune hepatitis (AIH) is a chronic inflammatory disorder with complex immunopathogenesis. Dysbiosis has been linked to many autoimmune diseases, but its detailed role in still needs rigorous evaluation, especially Egypt. We aimed identify the shift gut microbiota profile and resultant metabolic pathways AIH Egyptian patients compared healthy individuals. Stool samples were collected from 15 AIH-naive 10 The V3-V4 hyper-variable regions in16S rRNA gene was amplified sequenced using...

Radiotherapy-induced dermatitis (RID) is an inflammatory cutaneous disorder that acquired as adverse effect of undergoing radiotherapy. Skin microbiome dysbiosis has been linked to the outcomes several dermatological diseases. To explore skin microbiota RID and deduce their underlying impact on outcome RID, microbiomes 78 patients 20 healthy subjects were characterized by sequencing V1-V3 regions 16S rRNA gene. In total, a significantly apparent reduction in bacterial diversity was detected...

The feeding habit of Auchenoglanis biscutatus from Sebore Reservoir, Mayo–Belwa, Adamawa State was evaluated monthly for a period one year. A total 120 A. were collected as samples, washed with clean water and preserved in ice crest to minimize any post–mortem changes, taken the laboratory stomach contents analysis, while stomachs formalin solution about four per cent (4%). each sample identified analysed using numerical frequency occurrence methods. Five major groups food items constituted...

Hepatitis C virus is a hepatotropic that transmitted parenterally. Viral infections are usually associated with modulations of the immune cells, leading to enhanced viral survival and spreading, accordingly, life-threatening complications. Recently, it has been proposed new subset T-helper, named T-helper 9, involved in pathogenesis different immunopathological conditions, such as allergies, tumors, infections. Some studies reported protective role, others described pathogenic potential for...

A study was conducted at the Lay - Joy Fish Farm, Billiri, Gombe State Nigeria to evaluate carcass proximate composition of Clarias gariepinus fingerlings fed different levels Maca root powder as Phyto additive. Five (5) isonitrogenous (40% crude protein) and isocaloric (1,732kcal kg-1 gross energy) diets were formulated, where in form additive incorporated 0.0g/100g (TM0), 0.25g/100g (TM1), 0.5g/100g (TM2), 0.75g/100g (TM3) 1.0g/100g (TM4) inclusion levels, diet without served control diet....

Hepatitis E virus (HEV) causes about 14 million infections with 300,000 deaths and 5,200 stillbirths worldwide annually. Extrahepatic manifestations are reported HEV infections, such as renal, neurological, hematological disorders. Recently, we that stool-derived HEV-1 replicates efficiently in human monocytes macrophages vitro. However, another study reports the presence of viral RNA but no evidence replication PBMCs acute hepatitis (AHE) patients. Therefore, during AHE infection is not...

Purpose: To investigate the compositional and functional characteristics of T1DM-associated gut microbiota in two Egyptian cities to study geographical locality effects. Patients Methods: This case-control included 32 children with controlled T1DM 16 controls, selected from different regions Egypt. The both diabetic control was analyzed through 16S rRNA gene sequencing; this done using Illumina MiSeq platform. Results: Consistent findings among significantly lower alpha diversity than...

Objectives Meningitis is a medical emergency with permanent disabilities and high mortality worldwide. We aimed to determine causative microorganisms potential markers for differentiation between bacterial viral meningitis. Methodology Adult patients acute meningitis were subjected lumber puncture. Cerebrospinal fluid (CSF) identified using Real-time PCR. PCT CRP levels, peripheral CSF-leucocyte count, CSF-protein CSF-glucose levels assessed. Results Out of 80 patients, infectious was...

Detecting thyroid tumors depends on histologic characteristics. However, distinguishing malignant from benign abnormalities may be challenging and contentious, particularly in with a follicular appearance. Therefore, immunohistochemistry might useful essential. Immunohistochemical biomarkers, such as human trophoblast cell surface antigen (TROP) Hector Battifora Mesothelial-1 (HBME-1), have helped diagnose cancers. In addition, mesothelial cells an called HBME-1 their membranes, but its role...

Introduction: New Delhi metallo-β-lactamase (NDM)-producing K. pneumoniae poses a high risk, especially among Egyptian pediatric patients who consume carbapenems antibiotics very widely and without adequate diagnostic sources. In addition, presence of efflux pump genes such as OqxAB increases resistance against many groups antimicrobials which exacerbates the problem faced for human health. This study aimed to determine NDM variants strains isolated from in Egypt, analyze genes, molecular...

Direct-acting antivirals (DAAs) are used for hepatitis C virus (HCV) treatment. However, treatment failure and hepatocellular carcinoma (HCC) development following was reported. In this study, we assessed the role of serum vitamin D, interleukin 13 (IL-13), microRNA-135a in prediction with DAA HCC among Egyptian HCV-infected patients. A total 950 patients HCV-related chronic liver disease underwent Before DAAs, D IL-13 were determined by ELISA, gene expression miRNA-135a real-time PCR. The...

The effect of adding dried mushroom powder as a source antioxidants and fiber at level 1.0, 2.0 3.0% on physiochemical sensory properties low-fat yoghurt was studied. samples including pH values, titratable acidity, syneresis, viscosity, dietary fiber, total phenolic content, antioxidant activity were determined when fresh during storage for 15 days 5-7°C. Results showed gradual increase in content ratio increased. On the other hand, acidity synersis decreased Low- fat supplemented with up...

This study examines the hypothesis that tyrosine and tryptophan canthanthin either alone or in combination absence presence of sulphate ion, can improve performance local laying hens post-peak period (39-58 weeks). A total number 360 plus 36 cocks 39 weeks old from Inshas strain were equally divided into 12 group with 3 replicates (10 hens+ 1 each) housed wire cages . The experimental fed to 58 week age control diet without 6 ppm Canthaxanthin (CAN) , 0.05% tyrosine(TYR) tryptophan(TRY) CAN...

This research was carried out to determine the carcass essential amino acids profile of Clarias gariepinus fingerlings fed different levels Maca root powder as a feed additive. Five (5) isonitrogenous (40% crude protein) and isocaloric (1,732kcal kg-1 gross energy) diets were formulated, where in form additive included at 0.0g/100g (TM0), 0.25g/100g (TM1), 0.5g/100g (TM2), 0.75g/100g (TM3) 1.0g/100g (TM4) inclusion levels, diet without served control diet. Formulated (n = 300, 10.0±0.00g)...

This study was carried out at the Aquatic Bio - Resources Unit, National Biotechnology Development Agency, Katsina, Katsina State. One of objectives to evaluate cost benefit Clarias gariepinus fry fed dietary betaine hydrochloride based diet. Betaine incorporated in form a feed additive T0 (0.0g/100g,), T1 (0.25g/100g), T2 (0.5g/100g), T3 (0.75g/100g) and T4 (1.0g/100g) inclusion levels, respectively into 0.2 – 0.5mm size Coppens fish feeds containing 49% crude protein. Diet without served...

The food and feeding habits of Raiamas senegalensis from Chakawa Reservoir, Mayo – Belwa, Adamawa State was evaluated monthly for a period one year. A total 72 samples R. were collected, washed with clean water preserved in ice crest to minimize any post–mortem changes, taken the laboratory stomach contents analysis, where stomachs formalin solution about four percent (4%). Stomach each sample identified analysed using numerical frequency occurrence methods. diet constituted fish materials,...

Metode penelitian yang digunakan meliputi studi pustaka dan menghitung data secara langsung. Hasil menunjukan bahwa Pada produksi gula di PG Komering PT Lajuperdana Indah dari perhitungan analisa pabrik. diketahui nilai kalor paling rendah terdapat pada ke 3 dengan perolehan 1.799,4 kCal/kg dibandingkan 2 yaitu 1.914,6 1 tinggi angka 2.147,5 kCal/kg. Dari tersebut bisa mengetahui konsumsi bahan bakar. Data bakar 62.090,6 73.926,9 80.497,6. Semakin kadar semakin efesiensi boiler dibutuhkan....

Background: Spondylolysthesis represents a particular and relatively frequent mechanism of intervertebral instability.It could cause low back pain due to forward or anterior displacement one vertebra in relation the adjacent lower vertebra.There are many treatment interventions.However, there was no consensus on ideal approach. Aim work:To assess outcome trans-pedicular screw fixation with posterior inter-body fusion management lumber lumbosacral spondylolysthesis.Patients Methods: This is...

Objective The aim of this article was to focus on vitamin A level in obese children compared with normal controls and determine the relation between levels retinol lipid profile an inflammation marker. Participants methods Thirty 30 weight children, who served as controls, were included our study. All subjected clinical anthropometric evaluation. Laboratory assessment high-sensitivity C-reactive protein performed. Vitamin quantified. Results related profiles revealed that low-density...

ABSTRACTThe influence of gonadotropin releasing hormone (GnRH), Pregnant Mare SerumGonadotropin (PMSG) and human Chronic Gonadotropin (hCG) on some reproductive responses ofEgyptian ewe lambs was evaluated. Twenty five animals (aged 8 – 12 months weighed 27 - 33Kg) were divided into 5 groups ( each). Group (1) served as a control, (2),received vaginal sponges impregnated with MAP (60 mg medroxy progesterone acetate) for 14 days,during this period, fed 3 kg Berseem, (Trifolium alexandrinum)...