The global prevalence of COVID-19 disease and the overwhelming increase in death toll urge scientists to discover new effective drugs. Although drug discovery process is a challenging time-consuming, fortunately, plant kingdom was found have many active therapeutics possessing broad-spectrum antiviral activity including those candidates against severe acute respiratory syndrome coronaviruses (SARS-CoV). Herein, nine traditional Chinese medicinal constituents from different origins...

is a highly prevalent and aggressive human pathogen causing wide range of infections. This study aimed to explore the potential Patuletin, rare natural flavone, as an anti-virulence agent against

This study aimed to investigate the potential of patuletin, a rare natural flavonoid, as virulence and LasR inhibitor against Pseudomonas aeruginosa . Various computational studies were utilized explore binding Patuletin at molecular level. Molecular docking revealed that strongly interacted with active pocket LasR, high affinity value −20.96 kcal/mol. Further dynamics simulations, mechanics generalized Born surface area (MM/GBSA), protein-ligand interaction profile (PLIP), essential...

Quorum sensing inhibitionis an advanced strategy that aims to interfere with bacterial cell-to-cell communication systems (quorum sensing), which regulate virulence factors production in Pseudomonas aeruginosa, order overcome the globalcrisis of antimicrobial resistance.Study potential quorum inhibitory effect Zinc oxide (ZnO)nanoparticlesin aeruginosa and impact on factors.Quorum ZnO was evaluated by assessing its ability reducePseudomonas production; rhamnolipids, pyocyanin, pyoverdin,...

Radiotherapy-induced dermatitis (RID) is an inflammatory cutaneous disorder that acquired as adverse effect of undergoing radiotherapy. Skin microbiome dysbiosis has been linked to the outcomes several dermatological diseases. To explore skin microbiota RID and deduce their underlying impact on outcome RID, microbiomes 78 patients 20 healthy subjects were characterized by sequencing V1-V3 regions 16S rRNA gene. In total, a significantly apparent reduction in bacterial diversity was detected...

This study presents a green protocol for the fabrication of multifunctional smart nanobiocomposite (NBC) (ZnO-PIACSB-TiO

Abstract Background Infections affecting neonates caused by Staphylococcus aureus are widespread in healthcare facilities; hence, novel strategies needed to fight this pathogen. In study, we aimed investigate the effectiveness of FDA-approved medications ascorbic acid, dexamethasone, and sodium bicarbonate reduce virulence resistant bacteria that causes neonatal sepsis seek out suitable alternatives problem multi-drug resistance. Methods Tested drugs were assessed phenotypically...

Escherichia coli is one of the most ubiquitous pathogens causing several life-threatening diseases. Currently, antibiotics are failing fast while rates drug-resistant bacteria increasing worldwide with almost no new drugs coming to market and even last-resort becoming ineffective. Anti-virulence a approach targeting bacterial virulence unlike classic aimed at disarming rather than killing them or inhibiting their growth. Inactivation arsenal factors will attenuate render susceptible natural...

A comparison of the growth performance Penaeus vannamei was ascertained by supplementing potential probiotics isolated from a biofloc system incorporated through feed. Post-larvae shrimp (0.045 ± 0.005 g) were stocked at density 500/m3 in FRP tanks (500 L) triplicates for period 60 days. total 40 bacterial strains previous culture trials and tested their antimicrobial activity against pathogen Vibrio parahaemolyticus. Among these, Bacillus megaterium, Exiguobacterium profundum, Pseudomonas...

Pseudomonas aeruginosa is a standout amongst the most hazardous microorganisms of high morbidity and mortality rates especially in debilitated patients with few successful antibiotic choices available due to rapid dissemination antimicrobials resistance. Therefore, present study was intended examine susceptibility resistance pattern various clinical isolates taken from at an Egyptian hospital against commonly prescribed anti-pseudomonal antibiotics. In current study, 150 were recovered...

Escherichia coli is a Gram-negative bacterium that has the potential to be either harmful or beneficial as foodborne pathogen, nosocomial organism, part of normal microbiota. It one most prevalent pathogens in human population and life-threatening risk. primary causative agent urinary tract infections, gastrointestinal pneumonia, surgical site hemolytic uremic syndrome, sepsis, neonatal meningitis. The production virulence factors such biofilm, motility, serum resistance, protease enzymes by...

Quorum-sensing is a phenomenon of intercellular messages in microorganisms through secretion small signaling molecules, which spread between cells, facilitating communication, and regulating virulence factors production. system disruption Pseudomonas aeruginosa identifying new inhibitors promising approach. Herein, we test the possible quorum-sensing inhibitory effect esomeprazole, proton pump inhibitor, on standard strain PAO1. The production controlled violacein pigment biosensor...

The emergence of multidrug-resistant Klebsiella pneumoniae in hospitals represents a serious threat to public health. Infections caused by are widespread healthcare institutions, mainly pneumonia, bloodstream infections, and infections affecting neonates intensive care units; so, it is necessary combat this pathogen with new strategies. Targeting virulence factors induce host damage disease paradigm for antimicrobial therapy several potential benefits that could lead decreased resistance.The...

Background: Treatment of Klebsiella pneumoniae (K.pneumoniae) infections has become very difficult especially in the last decades as levels antibiotic resistance are becoming increasingly a serious problem throughout world.Aim: The growing number and rapid increase carbapenems which resort treatment K. urged for investigating some these possible mechanisms.Materials Methods: A total 202 isolates were selected from 593 specimens collected patients attended 4 hospitals during period October...

Pseudomonas aeruginosa is a Gram-negative bacterium frequently found in clinical specimens of hospitalized patients, mainly those with suppressed immune systems.It one the topmost ubiquitous nosocomial pathogens humans and considered life-threatening.Pseudomonas chief causative agent pneumonia, urinary tract infections, surgical site burns infections immunosuppressed patients.Pyocyanin, pyoverdin, exotoxin-A, rhamnolipids, protease enzymes are only few virulence factors produced by that...

Abstract Background : Infections affecting neonates caused by Staphylococcus aureus are widespread in healthcare facilities; hence, novel strategies needed to fight this pathogen. Targeting virulence factors necessary induce host damage and disease is a new tactic for antimicrobial therapy with several benefits which could lead decreased resistance. The effect of FDA-approved drugs (sodium bicarbonate, dexamethasone, ascorbic acid) sub-inhibitory concentration (1/8 MIC) was evaluated their...