A5042584522- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Child Nutrition and Water Access
- Diverse Scientific Research Studies
- Computational Drug Discovery Methods
- COVID-19 Clinical Research Studies
- Parasites and Host Interactions
- Respiratory viral infections research
- Global Maternal and Child Health
Institute of Human Virology
2021-2024
Serosurveillance can provide estimates of population-level exposure to infectious pathogens and has been used extensively during the COVID-19 pandemic. Simultaneous, serological testing for multiple be done using bead-based immunoassays add value disease-specific serosurveys. We conducted a validation four SARS-CoV-2 antigens-full-length spike protein, two receptor binding domain proteins, nucleocapsid protein-on our existing multiplex bead assay (MBA) enteric diseases, malaria, vaccine...
ABSTRACT. The first nationally representative, population-based study of schistosomiasis seroprevalence in Nigeria was conducted using blood samples and risk-factor data collected during the 2018 HIV/AIDS Indicator Impact Survey (NAIIS). Schistosomiasis estimated by analyzing for reactivity to schistosome soluble egg antigen (SEA) a multiplex bead assay; NAIIS survey were assessed identify potential risk factors seropositivity. SEA antibody available 31,459 children aged 0 14 years. Overall...
Objective There is a need for reliable serological assays to determine accurate estimates of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence. Most single target antigen have shown some limitations in Africa. To assess the performance multi-antigen assay, we evaluated commercially available SARS-CoV-2 Multi-Antigen IgG assay human disease 2019 (COVID-19) Nigeria. Methods Validation xMAP was carried out using well-characterized reverse transcription polymerase chain...
Multiplex assays for malaria antigen detection can gather data from large sample sets, but considerations the consistency and quality assurance (QA) of mass testing lack evaluation. We present a QA framework study occurring November 2019 to March 2020 involving 504 assay plates detecting four Plasmodium antigens: pan-Plasmodium aldolase lactate dehydrogenase (LDH), histidine-rich protein 2 (HRP2), P. vivax LDH (PvLDH). Controls on each plate included buffer blank, negative blood, 4-point...
Characterization of serological responses to Plasmodium falciparum (Pf) is interest understand disease burden and transmission dynamics; however, their interpretation challenging. Dried blood spots from 30,815 participants aged 6 months 15 years the 2018 Nigeria HIV/AIDS Indicator Impact Survey were analyzed by multiplex bead-based assay measure immunoglobulin G (IgG) Pf-stage-specific MSP-1, AMA-1, GLURPR0, LSA-1, CSP. These IgG levels principal component analysis (PCA). PC1 PC2 scores...