Abstract Intrinsic laryngeal muscles share many anatomical and physiological properties with extraocular muscles, which are unaffected in both Duchenne muscular dystrophy mdx mice. We hypothesized that intrinsic spared from myonecrosis mice may serve as an additional tool to understand the mechanisms of muscle sparing dystrophinopathy. tibialis anterior (TA) adult aged control C57Bl/10 were investigated. The percentage central nucleated fibers, a sign fibers had undergone injury...

The present study evaluated low-level laser therapy (LLLT) effects on some physiological pathways that may lead to muscle damage or regeneration capacity in dystrophin-deficient cells of mdx mice, the experimental model Duchenne muscular dystrophy (DMD). Primary cultures skeletal were irradiated only one time with and analyzed after 24 48 hours. LLLT parameter used was 830 nm wavelengths at 5 J/cm² fluence. following groups set up: Ctrl (untreated C57BL/10 primary cells), LA (mdx - h), h)....

The aim of this study was to characterize the structural and molecular biology as well evaluate immediate late responses prostatic cancer in transgenic adenocarcinoma mouse prostate (TRAMP) model after treatment with goniothalamin (GTN) celecoxib. treated mice received GTN (150 mg/kg, gavage) or celecoxib (10 from 8 12 weeks age. They were killed at different ages: immediate-response groups late-response 22 weeks. ventral collected for light microscopy, immunohistochemistry, western...

Increased oxidative stress is a frequent feature in Duchenne muscular dystrophy (DMD). High reactive oxygen species (ROS) levels, associated with altered enzyme antioxidant activity, have been reported dystrophic patients and mdx mice, an experimental model of DMD. In this study, we investigated the effects coenzyme Q10 (CoQ10) on marker levels calcium concentration primary cultures muscle cells from mice. Primary skeletal C57BL/10 mice were treated (5 μM) for 24 h. The untreated used as...

The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess Tempol effect dystrophic pathways, analyses in were performed blood samples and diaphragm muscle from trained adult (7–11-weeks old) animals. m dx mice divided into three groups: mdxSed , sedentary controls ( n = 28); Ex, exercise-trained animals Ex+T, with treatment 28). results demonstrated that promoted strength gain,...

ObjectivesOxidative stress and inflammatory process play an important role in the pathogenesis of Duchenne muscular dystrophy (DMD). We investigated whether deferoxamine (DFX) improves antioxidant effects N-acetylcysteine (NAC) on primary cultures dystrophic muscle cells from mdx mice, experimental model DMD.MethodsPrimary skeletal mice were treated with either NAC (10 mM), DFX (5 or plus for 24 hours. The C57BL/10 used as controls.ResultsProduction hydrogen peroxide (H2O2) levels...

Considering potential Tempol effects on mdx muscle fibers, in this study we evaluated its relevant dystrophic phenotypic characteristics, such as degeneration, inflammatory process and angiogenesis, which yet have not been investigated. Mdx mice were randomly assigned into three groups: mdxS, the control group receiving intraperitoneal (i.p.) injections of saline solution (100μL); mdxP, positive prednisolone (1mg/kg) by oral gavage; mdxT, treated i.p. tempol (100 mg/kg). C57BL/10 also used...

Abstract Background Brazilian berry is a fruit popularly known as “Jaboticaba,” rich in bioactive compounds with antioxidant and anti‐inflammatory properties. Senescence overweight are increasing worldwide considered risk factors to prostatic pathogenesis mainly due oxidative inflammatory processes induction. Thus, this study aimed evaluate the effect of two doses patented jaboticaba peel extract (PJE) on oxidative‐stress inflammation prostate aging or high‐fat‐fed mice. Methods PJE and/or...

Chronic inflammation and oxidative stress are striking features of Duchenne muscular dystrophy disease. Diacerhein is an anthraquinone, which exhibits anti-inflammatory antioxidant properties. Based on their actions, the present study evaluated effects diacerhein against myonecrosis, inflammatory response in diaphragm muscle mdx mice compared these results to current treatment widely used DMD patients, with a main focus impact prednisone. The demonstrated that prevented damage indicated by...

This study evaluated the possible protective effects of cilostazol against myonecrosis in dystrophic diaphragm muscle vivo, focusing on oxidative stress, inflammatory response and angiogenesis. Young mdx mice, experimental animal for Duchenne muscular dystrophy, received 14 days. A second group mice a control C57BL/10 saline solution. In treatment was associated with reduced loss strength (-34.4%), decreased myonecrosis, creatine kinase levels (-63.3%) fibres stained immunoglobulin G...

Abstract Oxidative stress is a critical element in relationship to the pathophysiology of Duchenne muscular dystrophy (DMD). In mice diaphragm (DIA) most resembles dystrophic human pathology. this study we have evaluated consequences synthetic antioxidant (tempol) on oxidative parameters DIA muscle mdx mice. The were separated into two groups: mdx, control group receiving intraperitoneal (i.p.) injections saline solution (100 µL), and mdxT, treated i.p. tempol mg/kg). tempol‐treated showed...

Abstract The muscle fiber phenotype is mainly determined by motoneuron innervation and changes in neuromuscular interaction alter the type. In dystrophin‐deficient mdx mice, molecular assembly of junction nerve terminal sprouting occur sternomastoid (STN) during early stages disease. this study, we were interested to see whether are correlated with alterations type dystrophic STN at 2 months age. A predominance hybrid fast myofibers (about 52% IIDB) was observed control (C57Bl/10) STN....

There is strong cross-talk between abnormal intracellular calcium concentration, high levels of reactive oxygen species (ROS) and an exacerbated inflammatory process in the dystrophic muscles mdx mice, experimental model Duchenne muscular dystrophy (DMD). In this study, we investigated effects Idebenone, a potent anti-oxidant, on oxidative stress markers, anti-oxidant defence system, concentrations primary muscle cells from mice. Dystrophic were treated with Idebenone (0.05 μM) for 24 h. The...

Changes in the distribution of acetylcholine receptors have been reported to occur at neuromuscular junction mdx mice and may be a consequence muscle fiber regeneration rather than absence dystrophin. In present study, we examined whether nerve terminal determines fate receptor dystrophic fibers mice. The left sternomastoid young (1-month-old) adult (6-month-old) was injected with 60 microl lidocaine hydrochloride induce degeneration-regeneration. Some had their denervated time injection....

Abstract In dystrophin‐deficient fibers of mdx mice and in Duchenne dystrophy, the lack dystrophin leads to sarcolemma breakdown muscle degeneration. We verified that cromolyn, a mast‐cell stabilizer agent, stabilized dystrophic using Evans blue dye as marker leakiness. Mdx ( n = 8; 14 days age) received daily intraperitoneal injections cromolyn (50 mg/kg body weight) for 15 days. Untreated 8) were injected with saline. Cryostat cross‐sections sternomastoid, tibialis anterior, diaphragm...

The commercial essential oil obtained from the steam distillation of cold-pressed Citrus aurantifolia (Christm.) Swingle fruits (OCA), after chemical characterization by gas chromatography-mass spectrometry (GC-MS), was investigated for antioxidant and anti-inflammatory activities related signaling pathways in dystrophic muscle cells. composition OCA showed 24 compounds with a predominance monoterpenes, such as limonene (42.6%), cymene (11.8%), α-terpineol terpinolene (5.3%) γ-terpinene...

We investigated whether the changes in acetylcholine receptor (AChR) distribution and neuronal nitric oxide synthase (nNOS) expression reported for skeletal muscle of mdx mice were a consequence fiber regeneration rather than absence dystrophin. Degenerative-regenerative fibers sternomastoid normal induced by injecting lidocaine hydrochloride. Twenty-one days later, AChRs labeled with alpha-bungarotoxin nNOS anti-nNOS antibody, observed under confocal microscope. distributed continuous...