The present study evaluated low-level laser therapy (LLLT) effects on some physiological pathways that may lead to muscle damage or regeneration capacity in dystrophin-deficient cells of mdx mice, the experimental model Duchenne muscular dystrophy (DMD). Primary cultures skeletal were irradiated only one time with and analyzed after 24 48 hours. LLLT parameter used was 830 nm wavelengths at 5 J/cm² fluence. following groups set up: Ctrl (untreated C57BL/10 primary cells), LA (mdx - h), h)....

The aim of this study was to characterize the structural and molecular biology as well evaluate immediate late responses prostatic cancer in transgenic adenocarcinoma mouse prostate (TRAMP) model after treatment with goniothalamin (GTN) celecoxib. treated mice received GTN (150 mg/kg, gavage) or celecoxib (10 from 8 12 weeks age. They were killed at different ages: immediate-response groups late-response 22 weeks. ventral collected for light microscopy, immunohistochemistry, western...

Increased oxidative stress is a frequent feature in Duchenne muscular dystrophy (DMD). High reactive oxygen species (ROS) levels, associated with altered enzyme antioxidant activity, have been reported dystrophic patients and mdx mice, an experimental model of DMD. In this study, we investigated the effects coenzyme Q10 (CoQ10) on marker levels calcium concentration primary cultures muscle cells from mice. Primary skeletal C57BL/10 mice were treated (5 μM) for 24 h. The untreated used as...

Considering potential Tempol effects on mdx muscle fibers, in this study we evaluated its relevant dystrophic phenotypic characteristics, such as degeneration, inflammatory process and angiogenesis, which yet have not been investigated. Mdx mice were randomly assigned into three groups: mdxS, the control group receiving intraperitoneal (i.p.) injections of saline solution (100μL); mdxP, positive prednisolone (1mg/kg) by oral gavage; mdxT, treated i.p. tempol (100 mg/kg). C57BL/10 also used...

O câncer colorretal está entre as neoplasias malignas que mais acometem pacientes no Brasil e mundo. Seu diagnóstico precoce permite um tratamento eficaz prognóstico muito bom para o paciente, mas é dificultado pela generalidade dos sinais sintomas ligados ao colorretal, uma vez também estão relacionados a outras patologias. Sua fisiopatologia apresenta características de desenvolvimento semelhantes da maioria das lesões neoplásicas, com seu silencioso dependendo localização lesão, pode...

Chronic inflammation and oxidative stress are striking features of Duchenne muscular dystrophy disease. Diacerhein is an anthraquinone, which exhibits anti-inflammatory antioxidant properties. Based on their actions, the present study evaluated effects diacerhein against myonecrosis, inflammatory response in diaphragm muscle mdx mice compared these results to current treatment widely used DMD patients, with a main focus impact prednisone. The demonstrated that prevented damage indicated by...

This study evaluated the possible protective effects of cilostazol against myonecrosis in dystrophic diaphragm muscle vivo, focusing on oxidative stress, inflammatory response and angiogenesis. Young mdx mice, experimental animal for Duchenne muscular dystrophy, received 14 days. A second group mice a control C57BL/10 saline solution. In treatment was associated with reduced loss strength (-34.4%), decreased myonecrosis, creatine kinase levels (-63.3%) fibres stained immunoglobulin G...

The aging process and its associated morphophysiological changes trigger a reduction in the regenerative ability of satellite cells, vascular tissue an increase production fibroblasts, developing cellular environment unfavorable for muscle regeneration.The aim this study was to evaluate effect low-level laser therapy on regeneration old experimental rat models after contusion.A total 25 rats,18 months old, were divided into three groups: control group (CT) without treatment; injury (IN) with...

There is strong cross-talk between abnormal intracellular calcium concentration, high levels of reactive oxygen species (ROS) and an exacerbated inflammatory process in the dystrophic muscles mdx mice, experimental model Duchenne muscular dystrophy (DMD). In this study, we investigated effects Idebenone, a potent anti-oxidant, on oxidative stress markers, anti-oxidant defence system, concentrations primary muscle cells from mice. Dystrophic were treated with Idebenone (0.05 μM) for 24 h. The...

The commercial essential oil obtained from the steam distillation of cold-pressed Citrus aurantifolia (Christm.) Swingle fruits (OCA), after chemical characterization by gas chromatography-mass spectrometry (GC-MS), was investigated for antioxidant and anti-inflammatory activities related signaling pathways in dystrophic muscle cells. composition OCA showed 24 compounds with a predominance monoterpenes, such as limonene (42.6%), cymene (11.8%), α-terpineol terpinolene (5.3%) γ-terpinene...

Duchenne muscular dystrophy (DMD) is the most severe and frequent form of dystrophy. The mdx mouse one widely used experimental models to understand aspects biology dystrophic skeletal muscles mechanisms DMD. Oxidative stress apoptosis are present in early stages disease mice. high production reactive oxygen species (ROS) causes activation apoptotic death regulatory proteins due DNA damage breakdown nuclear mitochondrial membranes. quadriceps (QUA) muscle a good tool study oxidative events....

Este estudo teve o objetivo de investigar se há diferenças entre as terapias associadas e isoladas do laser microcorrentes no reparo lesão por queimadura em ratos. Um total 40 animais foi dividido aleatoriamente quatro grupos: grupo controle (GC); microcorrente (GM), (GL) laser/microcorrente (GLM), tratados com associado a microcorrentes. Após lesões térmicas induzidas dorso animal, realizado um dez dias tratamento. Amostras tecido foram coletadas para histopatológico semiquantitativo...

Abstract This study analyzed photobiomodulation therapy (PBMT) effects on regenerative, antioxidative, anti‐inflammatory and angiogenic markers in the dystrophic skeletal muscle of mdx mice, experimental model Duchenne muscular dystrophy (DMD), during acute phase disease. The following groups were set up: Ctrl (control group normal wild‐type mice; C57BL/10); (untreated mice); Pred ( mice treated with prednisolone) LA PBMT). PBMT was carried out using an Aluminum Gallium Arsenide (AIGaAs;...

This study investigated and compared the effects of low-level laser therapy (LLLT) microcurrent in burn healing process Wistar rats. We conducted a randomized controlled with 30 rats divided into 3 groups (n = 10); control group (CG), (LG) (MG). After thermal damage, 10 applications 660 nm diode were performed GL 60 Hz (160 μA) MG. The semi-quantitative histological analysis was done using scores (0–3), sections stained by hematoxylin eosin Masson’s trichrome. results indicated significant...

Physical exercise promotes increased muscle damage in the mdx mice, experimental model of Duchenne muscular dystrophy. Studies suggest that estrogen level females makes them less susceptible to injuries. The aim this study was characterize diaphragm (DIA) response physical male and female mice. animals were divided into four groups: sedentary mdx; submitted exercise; mice exercise. Blood samples used determine creatine kinase (CK). Regenerated fibers indicated by presence central nucleus...

Manipulation of the spinal column is a manual therapeutic resource characterized by passive thrust given joint at high velocity and low amplitude within limits anatomic integrity. The objective present study was to assess immediate effects upper thoracic manipulation on skin temperature in vertebral region healthy women. Thus, randomized controlled blind trial realized university community. Twenty-six women were randomly allocated into an experimental group (n=13) placebo (n=13). A single...

The relationship of external organs located in the female perineum, such as ostium urethra and anus, associated with ineffective practices intimate hygiene, predisposes urinary tract infections (UTIs) vulvovaginitis women, reaching, each year, more than 150 million. Knowledge a form prevention these pathologies, well awareness population, is an important tool public health. objective this work was to elaborate didactic material educational poster, focused at children adolescents, order...

Diante do aumento da expectativa de vida população idosa no Brasil e mundo, são inúmeras as discussões acerca qualidade condição desses indivíduos. O envelhecimento é um processo gradual, universal irreversível, provocando perda funcional progressiva organismo. complexo varia idoso para idoso, reunindo diversas alterações nas capacidades: equilíbrio, mobilidade, fisiológicas, articulares psicológicas. Desta forma o presente estudo teve como objetivo investigar principais relacionadas com...

Antioxidant treatments showed beneficial effects in dystrophic skeletal muscles Duchenne muscular dystrophy patients and the experimental model, mdx mice. Due to fact that studies suggest a combination of N-Acetylcysteine (NAC) antioxidant Deferoxamine (DFX) iron chelator is an effective treatment for several oxidative stress diseases, this study our aim was investigate vivo potential effect NAC DFX Efeitos de terapia combinada antioxidante e quelante ferro no músculo esquelético camundongos...

To apprehend the Human Anatomy, is nothing more than understanding of constituent structures human body and its macro microscopic organization, visualized through cuts.The teaching Anatomy carried out from study reading memorization that make up individual, latter being ineffective for student learning. The didactic resources applied to discipline, shown as a satisfactory trend stimulates participation an active subject in search new information, giving indispensable support - learning...