A5079352050- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Immune cells in cancer
- IL-33, ST2, and ILC Pathways
- Acute Lymphoblastic Leukemia research
- Erythrocyte Function and Pathophysiology
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Plant and animal studies
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Microfluidic and Bio-sensing Technologies
- Genetic diversity and population structure
- Spaceflight effects on biology
- High Altitude and Hypoxia
- Alkaline Phosphatase Research Studies
- Essential Oils and Antimicrobial Activity
- Soil Carbon and Nitrogen Dynamics
- Biomarkers in Disease Mechanisms
- Multiple Myeloma Research and Treatments
- Obesity, Physical Activity, Diet
Universitat Autònoma de Barcelona
2008-2024
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2019-2024
Science Council
2024
Josep Carreras Leukaemia Research Institute
2016-2021
Molina Center for Energy and the Environment
2020
Universitat Oberta de Catalunya
2018-2019
Centre for Research on Ecology and Forestry Applications
2007-2017
Andalusian Agency for Healthcare Quality
2017
Global Ecology Unit CREAF-CSIC-UAB
2014-2016
Institute of Photonic Sciences
2013
<h3>Background and aims:</h3> Inflammatory bowel diseases (IBDs) are associated with uncontrolled innate adaptive immunity against normal constituents, including commensal bacteria microbial products. Mesenchymal stem cells (MSCs) suppress effector T cell responses have beneficial effects in various immune disorders. This work investigates the therapeutic of human adipose-derived MSCs (hASCs) models IBD sepsis. <h3>Methods:</h3> Acute chronic colitis was induced mice dextran sulfate sodium....
Abstract Objective Rheumatoid arthritis (RA) is a chronic autoimmune disease caused by loss of immunologic self tolerance and characterized joint inflammation. Adult mesenchymal stem cells (MSCs) were recently found to suppress effector T cell responses have beneficial effects in various immune disorders. The purpose this study was examine new therapeutic strategy for RA based on the administration human adipose‐derived MSCs (AD‐MSCs). Methods DBA/1 mice with collagen‐induced treated AD‐MSCs...
Objectives: Adult mesenchymal stem cells were recently found to suppress effector T cell and inflammatory responses have emerged as attractive therapeutic candidates for immune disorders. In rheumatoid arthritis (RA), a loss in the immunological self-tolerance causes activation of autoreactive against joint components subsequent chronic inflammation. The aim this study is characterise immunosuppressive activity human adipose-derived (hASCs) on collagen-reactive from patients with RA....
Human adipose–derived mesenchymal stem cells (hASCs) are (MSCs) with reduced immunogenicity and capability to modulate immune responses. Whereas the immunosuppressive activity of bone marrow–MSCs has received considerable attention during last few years, specific mechanisms underlying hASC-mediated immunosuppression have been poorly studied. Recent studies comparing both cell types reported differences at transcriptional proteomic levels, suggesting that hASCs marrow–MSCs, while having...
Abstract We explored the changes in richness, diversity and evenness of epiphytic (on leaf surface) endophytic (within tissues) bacteria fungi foliar phyllosphere Quercus ilex, dominant tree species Mediterranean forests. Bacteria were assessed during ontogenic development leaves, from wet spring to dry summer season control plots subjected drought conditions mimicking those projected for future decades. Our aim was monitor succession microbiota colonisation plant leaves its response climate...
Plastic pollution is a global problem. Animals and humans can ingest inhale plastic particles, with uncertain health consequences. Nanoplastics (NPs) are particles ranging from 1 nm to 1000 that result the erosion or breakage of larger debris, be highly polydisperse in physical properties heterogeneous composition. Potential effects NPs exposure may associated alterations xenobiotic metabolism, nutrients absorption, energy cytotoxicity, behavior. In humans, no data on absorptions has been...
Rapid genetic changes in plants have been reported response to current climate change. We assessed the capacity of trees a natural forest produce rapid acclimation responses based on epigenetic modifications. analysed populations Quercus ilex, dominant tree species Mediterranean forests, using methylation-sensitive amplified polymorphism (MSAP) technique assess patterns and levels methylation individuals from unstressed plots experimentally exposed drought for 12 years at projected coming...
Controlling and monitoring temperature at the single cell level has become pivotal in biology medicine. Indeed, influences many intracellular processes is also involved as an activator novel therapies. Aiming to assist such developments, several approaches have recently been proposed probe vitro. None of them so far extended a living organism. Here we present first vivo imaging. Our technique relies on measuring fluorescence polarization anisotropy green fluorescent protein (GFP) set GFP...
The emission of floral terpenes plays a key role in pollination many plant species. We hypothesized that the phyllospheric microbiota could significantly influence these terpene emissions because microorganisms also produce and emit terpenes. tested this hypothesis by analyzing effect removing from flowers. fumigated Sambucus nigra L. plants, including their flowers, with combination three broad-spectrum antibiotics measured tissular concentrations both antibiotic-fumigated non-fumigated...
Abstract Rapid climate change will impose strong directional selection pressures on natural plant populations. Climate‐linked genetic variation in populations indicates that an evolutionary response is possible. We investigated such a by comparing individuals subjected to elevated drought and warming treatments with establishing unmanipulated within the same population. report reduction seedling establishment manipulations nonrandom results from pressure imposed artificially warmed droughted...
Abstract Biological nitrogen ( N ) fixation is a key pathway in terrestrial ecosystems and therefore critical for understanding the responses of to global environmental changes. The free‐living diazotrophic community distributed along canopy‐to‐soil profile, but ecological significance epiphyllic 2 fixers, despite their functional relevance, on plant foliar surfaces remains very poorly understood compared with ‐fixing forest litter soils. We assessed structure fixers overall bacteria by...
Predicting the risks of permanent gene therapy approaches involving use integrative gene-targeting vectors has become a critical issue after unfortunate episode clinical trial in children with X-linked severe combined immunodeficiency (X-SCID). Safety pre-assessment single isolated gene-targeted stem cells or their derivative clones able to regenerate tissue origin would be major asset addressing untoward effects advance. Human epidermal cells, which have extensive proliferative potential...
The phyllospheric microbiota is assumed to play a key role in the metabolism of host plants. Its determining epiphytic and internal plant metabolome, however, remains be investigated. We analyzed Liquid Chromatography-Mass Spectrometry (LC-MS) profiles metabolomes leaves flowers Sambucus nigra with without external antibiotic treatment application.The showed degree complexity similar that organs. suppression microbial communities by topical applications antibiotics had greater impact on...
Summary Ageing and senescence in plants remain poorly understood. Although meristem totipotency may allow woody perennials to be immortal, relative growth photosynthetic rates typically decline with age. Trees of ages between 129 534 years were selected one the oldest extant populations Scots pine. Apical branches propagated by grafting onto homogeneous juvenile rootstock eliminate effects size environmental variability isolate those due The hormonal profile leaves seeds along markers...
This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which have been successfully used identify healthy pathologically rare cells. The greatest advantage these is that they minimize the unwanted effect caused preparation, allowing improved study live at point...
Leukemic stem cells (LSCs) possess similar characteristics to normal hematopoietic cells, including self-renewal capacity, quiescence, ability initiate leukemia, and drug resistance. These play a significant role in leukemia relapse, persisting even after apparent remission. LSCs were first described 1994 by Lapidot et al. Although they have been extensively studied acute more LSC research is still needed chronic lymphocytic (CLL) understand if reduced apoptosis mature should be considered...
Even though anti-PD-1/PD-L1 immune checkpoint inhibitors (ICIs) in non-small cell lung cancer (NSCLC) have improved survival, a high percentage of patients still do not respond to ICIs. Myeloid-derived suppressor cells (MDSCs) are circulating that express PD-L1 and can infiltrate proliferate the tumor microenvironment, inducing immunosuppression. By evaluating changes expression live peripheral blood MDSCs, we able define new index, useful predicting ICI escape NSCLC before initiating...
With the aim to detect candidate malignant primitive progenitor populations, we modified an original alkaline phosphatase (ALP) stem cell detection method based on identification of fluorescent cells in combination with flow cytometry immunophenotyping. Over a period one year, have been using this technique study its activity patients leukemia and lymphoma, showing that changes levels can be used rare populations highly refractory cells. By screening different blood cancers, observed is not...