A5009839543- Cervical Cancer and HPV Research
- Cancer-related molecular mechanisms research
- Endometrial and Cervical Cancer Treatments
- Genital Health and Disease
- Reproductive tract infections research
- Epigenetics and DNA Methylation
- Endometriosis Research and Treatment
- Gestational Trophoblastic Disease Studies
- Global Cancer Incidence and Screening
- Colorectal Cancer Screening and Detection
- Urinary and Genital Oncology Studies
- Prenatal Screening and Diagnostics
- Uterine Myomas and Treatments
- RNA modifications and cancer
- Ectopic Pregnancy Diagnosis and Management
- Molecular Biology Techniques and Applications
- AI in cancer detection
Vrije Universiteit Amsterdam
2020-2024
Amsterdam University Medical Centers
2020-2024
Dutch Cancer Society
2022-2024
Cancer Center Amsterdam
2022-2024
Amsterdam University of Applied Sciences
2023
Norwegian Womens Public Health Association
2023
The Netherlands Cancer Institute
2018
University of Amsterdam
2017
Abstract STUDY QUESTION Does incisional endometriosis (IE) harbor somatic cancer-driver mutations? SUMMARY ANSWER We found that approximately one-quarter of IE cases somatic-cancer mutations, which commonly affect components the MAPK/RAS or PI3K-Akt-mTor signaling pathways. WHAT IS KNOWN ALREADY Despite classification as a benign gynecological disease, it shares key features with cancers such resistance to apoptosis and stimulation angiogenesis is well-established precursor clear cell...
Abstract Endometrial cancer incidence is rising and current diagnostics often require invasive biopsy procedures. DNA methylation marker analysis of minimally‐ non‐invasive sample types could provide an easy‐to‐apply patient‐friendly alternative to determine risk. Here, we compared the performance markers detect endometrial in urine, cervicovaginal self‐samples clinician‐taken cervical scrapes. Paired samples were collected from 103 patients diagnosed with stage I IV cancer. Urine at home....
Cervical cancer screening programs, including triage tests, need redesigning as human papillomavirus (HPV)-vaccinated women are entering the programs. Methylation markers offer a potential solution to reduce false-positive rates by identifying clinically relevant cervical lesions with progressive potential. In nested case-control study, 9242 who received three-dose HPV16/18-vaccine at ages 12-15 or 18 in community-randomized trial were included. Subsequently, they re-randomized for either...
Methylation of host-cell deoxyribonucleic acid (DNA) has been proposed as a promising biomarker for triage high-risk (hr) human papillomavirus (HPV) positive women at screening. Our study aims to validate recently identified DNA methylation markers in an hrHPV-positive cohort derived from primary HPV-based cervical screening The Netherlands. ASCL1, LHX8, ST6GALNAC5, GHSR, ZIC1 and SST were evaluated relative the ACTB reference gene by multiplex quantitative methylation-specific PCR (qMSP)...
Abstract Background Host-cell DNA methylation analysis can be used to triage women with high-risk human papillomavirus (HPV)-positive self-collected cervicovaginal samples, but current data are restricted under-/never-screened and referral populations. This study evaluated performance in who were offered primary HPV self-sampling for cervical cancer screening. Methods Self-collected samples from 593 HPV-positive participated a trial (IMPROVE study; NTR5078), tested the markers ASCL1 LHX8...
Aim: To evaluate the triage performance of six host-cell DNA methylation markers derived from two genome-wide discovery screens for detection cervical precancer (cervical intraepithelial neoplasia 3 [CIN]) and cancer. Materials & methods: Human papillomavirus-positive scrapes controls (≤CIN1; n = 352) women diagnosed with CIN3 (n 175) or cancer 50) were analyzed ASCL1, LHX8, ST6GALNAC5, GHSR, SST ZIC1. Results: Methylation levels increased significantly disease severity (all p < 0.001)....
Human papillomavirus (HPV) testing on self-samples is a valid tool for cervical cancer screening. HPV self-sample workflows need to be clinically validated ensure safe use in This study evaluated the fully automated NeuMoDx Assay workflow that compiled of assay and 96/288 Molecular Systems, clinical performance reproducibility Evalyn Brush-collected self-samples. The intraepithelial neoplasia grade 2 or worse (CIN2+) CIN3+ was 987 obtained from women attending national organized HPV-based...
Endometriosis is a common gynecological disease and precursor of clear cell endometrioid ovarian carcinomas. We recently demonstrated that one form endometriosis with little malignant potential, deep infiltrating (DE), harbours recurrent somatic cancer-driver mutations. The prevalence these mutations in other forms not known. sought to investigate this by examining unlikely undergo transformation. Particularly, we examined an iatrogenic (incisional endometriosis; IE) where the uterine origin...
Gestational trophoblastic neoplasia (GTN) is classified as a highly curable group of pregnancy-related malignancies; however, approximately 15% will be persistent and require chemotherapy. Up to 25% these women develop resistance 2% disease relapse after initial Despite the need for further chemotherapy in women, cure rates are high.To evaluate outcomes diagnosed with low-risk GTN, assessing type treatment, number cycles received, development or relapse, survival, assess feasibility changing...
Sodium bisulphite conversion of DNA to separate methylated from unmethylated cytosines is a standard for methylation analysis. This study evaluated direct cell protocol on cervical samples as alternative isolated genomic input.Clinician-collected (n = 120) were subjected protocol, or was with fixed amount used subsequent conversion. Converted compared ACTB control gene and FAM19A4 miR124-2 genes using quantitative methylation-specific PCR (QIAsure Methylation Test).Direct resulted in high...
Abstract High‐risk HPV (hrHPV)‐based screening has led to many unnecessary colposcopy referrals, mainly because of direct referral after low‐grade cytology (ASC‐US/LSIL). DNA methylation and genotyping tests on ASC‐US/LSIL samples have the potential significantly improve efficiency screening. In this study, 12 triage strategies were constructed from FAM19A4/miR124‐2 or ASCL1/LHX8 methylation, HPV16/18 HPV16/18/31/33/45 1‐year repeat cytology. The performance was evaluated 215 hrHPV‐positive...
ABSTRACT Endometrial cancer is the sixth most common in women globally and has a rising incidence, accounting for 417,000 new diagnoses more than 97,000 deaths 2020. Early detection critical because of poor advanced-stage prognosis high relapse risk. Postmenopausal bleeding precedes endometrial 90% cases, yet only 5% to 10% patients with this symptom have an underlying malignancy. Recent cytology research demonstrated cells vaginal samples, suggesting urine cervicovaginal self-samples may...