A5065920319- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Cell Image Analysis Techniques
- Neonatal and fetal brain pathology
- Advanced Fluorescence Microscopy Techniques
- 3D Printing in Biomedical Research
- Receptor Mechanisms and Signaling
- Neuroscience of respiration and sleep
- Anesthesia and Neurotoxicity Research
- MicroRNA in disease regulation
- Thermal Regulation in Medicine
- Fetal and Pediatric Neurological Disorders
- Neurotransmitter Receptor Influence on Behavior
- Bone Tissue Engineering Materials
- Neuroendocrine regulation and behavior
- Retinal Development and Disorders
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
Massachusetts Institute of Technology
2018-2024
Kennedy Krieger Institute
2017-2018
Abstract The brainstem region, locus coeruleus (LC), has been remarkably conserved across vertebrates. Evolution woven the LC into wide-ranging neural circuits that influence functions as broad autonomic systems, stress response, nociception, sleep, and high-level cognition among others. Given this conservation, there is a strong possibility activity inherently similar species, furthermore age, sex, brain state similarly species. degree to which homogenous these factors, however, never...
Abstract Here we describe an image processing pipeline for quantitative analysis of terabyte-scale volumetric images SHIELD-processed mouse brains imaged with light-sheet microscopy. The utilizes open-source packages destriping, stitching, and atlas alignment that are optimized parallel processing. destriping step removes stripe artifacts, corrects uneven illumination, offers over 100x speed improvements compared to previously reported algorithms. stitching module builds upon Terastitcher...
Perinatal hypoxic ischemic encephalopathy (HIE) can lead to neurodevelopmental disorders, including cerebral palsy. Standard care for neonatal HIE includes therapeutic hypothermia, which provides partial neuroprotection; magnetic resonance imaging (MRI) is often used assess injury and predict outcome after HIE. Immature rodent models of are evaluate mechanisms examine the efficacy neuroprotective interventions such as hypothermia. In this study, we first confirmed that, in CD1 mouse model...
Updating behavior based on feedback from the environment is a crucial means by which organisms learn and develop optimal behavioral strategies
Summary Astrocytes, which are increasingly recognized as pivotal constituents of brain circuits governing a wide range functions, express GABA transporter 3 (Gat3), an astrocyte-specific responsible for maintenance extra-synaptic levels. Here, we examined the functional role Gat3 in astrocyte-mediated modulation neuronal activity and information encoding. First, developed multiplexed CRISPR construct applicable effective genetic ablation visual cortex adult mice. Using vivo two-photon...