A5077756288- Natural Antidiabetic Agents Studies
- Pancreatic function and diabetes
- Phytochemicals and Antioxidant Activities
- Coenzyme Q10 studies and effects
- Natural product bioactivities and synthesis
- 3D Printing in Biomedical Research
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Advanced battery technologies research
- Regulation of Appetite and Obesity
- Genomics, phytochemicals, and oxidative stress
- Advanced Battery Technologies Research
- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Chromatography in Natural Products
- Diet and metabolism studies
- Pluripotent Stem Cells Research
- Metabolism, Diabetes, and Cancer
- Cardiac Ischemia and Reperfusion
- Global Public Health Policies and Epidemiology
- Phytochemical Studies and Bioactivities
- Biochemical Acid Research Studies
- Liver physiology and pathology
- Chemotherapy-induced organ toxicity mitigation
- Ginkgo biloba and Cashew Applications
South African Medical Research Council
2014-2024
Stellenbosch University
2012-2024
Medical Research Council
2008
Although numerous studies have demonstrated the biological and multifaceted nature of dimethyl sulfoxide (DMSO) across different in vitro models, direct effect "non-toxic" low DMSO doses on cardiac cancer cells has not been clearly explored. In present study, H9c2 cardiomyoblasts MCF-7 breast were treated with varying concentrations (0.001-3.7%) for 6 days. Here, < 0.5% enhanced respiratory control ratio cellular viability relative to cells. However, 3.7% exposure rate apoptosis, which was...
Scope The rare enolic phenylpyruvic acid‐2‐ O ‐glucoside, (PPAG: Z ‐2‐(β‐ d ‐glucopyranosyloxy)‐3‐phenylpropenoic acid), is one of the major constituents fermented rooibos infusions. 3‐Phenylpyruvic acid (2‐oxo‐3‐phenylpropanoic without sugar moiety and with a keto form instead an arrangement, has been shown to enhance insulin release glucose uptake in muscle cells. purpose this study was assess if PPAG similar activity on metabolism. Methods results Preliminary vitro studies confirmed that...
A major goal of diabetes therapy is to identify novel drugs that preserve or expand pancreatic beta cell mass. Here, we examined the effect a phenylpropenoic acid glucoside (PPAG) on mass, and via which mechanism this established.Mice were fed high-fat fructose-containing diet induce obesity hyperglycemia. PPAG treatment protected obese mice from diet-induced hyperglycemia resulted in tripling The phytochemical mass was neither due increased proliferation, as determined by Ki67...
Type 2 diabetic patients possess a two to four-fold-increased risk for cardiovascular diseases (CVD). Hyperglycemia, oxidative stress associated with endothelial dysfunction and dyslipidemia are regarded as pro-atherogenic mechanisms of CVD. In this study, high-fat diet-induced non-diabetic vervet monkeys were treated 90 mg/kg aspalathin-rich green rooibos extract (Afriplex GRT) 28 days, followed by 1-month wash-out period. Supplementation showed improvements in both the intravenous glucose...
New strategies, which include <i>β</i>-cell protection, are required in the treatment of T2D, as current drugs demonstrate little or no capacity to directly protect vulnerable against diabetes-induced cytotoxicity. In this study we investigated ameliorative effect pre-treatment with an aqueous extract unfermented <i>Cyclopia maculata</i> (honeybush) on STZ-induced diabetes and pancreatic cytotoxicity Wistar rats after demonstrating a protective <i>in vitro</i> RIN-5F cells. The amelioration...
Non-communicable diseases contribute significantly to the disease burden within South Africa. In most unequal of societies in world, poverty and socio-economic disparity are amongst greatest obstacles facing Africans, impacting heavily on health care. Adverse socio-environmental factors, especially those experienced during early life, can, through neurobiological epigenetic mechanisms, developmentally programme outcome obesity, diabetes, cardiovascular mental disorders adulthood. this...
Recent evidence shows that rooibos compounds, aspalathin and phenylpyruvic acid-2-O-β-d-glucoside (PPAG), can independently protect cardiomyocytes from hyperglycemia-related reactive oxygen species (ROS). While more potency by enhancing intracellular antioxidant defenses, PPAG acts as an anti-apoptotic agent. Thus, to further understand the protective capabilities of these compounds against hyperglycemia-induced cardiac damage, their combinatory effect was investigated compared metformin. An...
Over the years, immortalized rodent β-cell lines such as RIN, HIT, MIN, βTC, and INS-1 have been used to investigate pancreatic physiology using conventional two-dimensional (2D) culture techniques. However, physical physiological limitations inherent 2D cell necessitates confirmatory follow up studies sentient animals. Three-dimensional (3D) models are gaining popularity for their recapitulation of key features in vivo organ physiology, thus could pose potential surrogates animal...
Rooibos extracts, including its derived polyphenolic compounds, are increasingly explored for their ameliorative effects against diabetes-associated complications. An extract of 'fermented' (oxidized) rooibos (FRE) can protect cardiomyocytes from diabetic rats oxidative stress-induced damage, however, mechanism action remains unknown. Using an established model H9c2 exposed to high glucose concentrations, the current study elaborates on mechanisms associated with cardioprotective FRE,...
Athrixia phylicoides is an aromatic, indigenous shrub used to brew “bush tea.” Surveys have shown that the consumption of bush tea widespread and commercialization extract holds economic developmental potential. Aqueous extracts A. are non-toxic brine shrimp renal cell lines; however, verification in a mammalian model needed. In this study, daily ingestion high doses aqueous by mature Wistar rats was tested for potential toxicity over 3-month period. Three-month-old were randomized into...
Background. This study evaluated in vitro antidiabetic and antioxidant properties of different extracts (n-hexane, dichloromethane (DCM), 70% ethanol) honeybush tea (Cyclopia genistoides). Over a period 28 days, antiprotein glycation was some indicators (α-amylase, α-glucosidase, pancreatic lipase inhibitory effects) activities (DPPH, ABTS, hydroxyl radical, metal ion chelating, reducing power) for each the crude were also investigated. The results showed that all tested C. genistoides had...