A5087430277- Trace Elements in Health
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Connexins and lens biology
- Iron Metabolism and Disorders
- Advanced Nanomaterials in Catalysis
- Mercury impact and mitigation studies
- Bioenergy crop production and management
- Nanocluster Synthesis and Applications
- Retinal Imaging and Analysis
- Heavy Metal Exposure and Toxicity
- Blood Coagulation and Thrombosis Mechanisms
- Retinoids in leukemia and cellular processes
- Machine Learning in Bioinformatics
- S100 Proteins and Annexins
- Analytical Chemistry and Chromatography
- Retinal Development and Disorders
- Plant Reproductive Biology
- Neurological Complications and Syndromes
- Barrier Structure and Function Studies
- Alcohol Consumption and Health Effects
- Microbial metabolism and enzyme function
- Neurological Disorders and Treatments
- Medical Coding and Health Information
- Intraocular Surgery and Lenses
Fernández-Vega Ophthalmological Institute
2016-2025
Universidad de Oviedo
1985-2023
Instituto Dermatológico y Cirugía de Piel "Dr. Huberto Bogaert Díaz"
2017-2020
Fundación Neumológica Colombiana
2017
In-Q-Tel
2013
Umeå Plant Science Centre
2007
The initiation of growth cessation and dormancy represents a critical ecological evolutionary trade-off between survival in most forest trees. important environmental cue regulating the is shortening photoperiod phytochrome genes have been implicated short-day-induced bud set Populus. We characterized patterns DNA sequence variation at putative candidate gene phyB2 4 populations European aspen (Populus tremula) scored single-nucleotide polymorphisms an additional 12 collected along...
We investigated the utility of association mapping to dissect genetic basis naturally occurring variation in bud phenology European aspen (Populus tremula). With this aim, we surveyed nucleotide polymorphism 13 fragments spanning an 80-kb region surrounding phytochrome B2 (phyB2) locus. Although varies substantially across phyB2 region, detected no signs for deviations from neutral expectations. also identified a total 41 single polymorphisms (SNPs) that were subsequently scored population...
Oxidative stress affects all the structures of human eye, particularly retina and its retinal pigment epithelium (RPE). The RPE limits oxidative damage by several protective mechanisms, including non-enzymatic antioxidant system zinc-metallothionein (Zn-MT). This work aimed to investigate role Zn-MT in protection from reactive oxygen intermediates analytical biochemical-based techniques. was induced an vitro model cells determined elemental mass spectrometry with enriched isotopes...
Selenium supplementation protects retinal pigment epithelial cells from glucose-induced stress by preserving glutathione peroxidase activity.
<h3>Importance</h3> Exfoliation syndrome is a systemic disorder characterized by progressive accumulation of abnormal fibrillar protein aggregates manifesting clinically in the anterior chamber eye. This most commonly known cause glaucoma and major irreversible blindness. <h3>Objective</h3> To determine if exfoliation associated with rare, protein-changing variants predicted to impair function. <h3>Design, Setting, Participants</h3> A 2-stage, case-control, whole-exome sequencing association...
Age-related macular degeneration (AMD) is a leading cause of blindness, characterized by retinal pigment epithelium (RPE) dysfunction, extracellular deposit formation, and disrupted lipid metabolism. Understanding the molecular changes underlying AMD essential for identifying diagnostic markers therapeutic targets. This multiomic study employed primary RPE culture model to investigate age-related associated with AMD. Over 25 weeks, cells exhibited phenotypic deterioration, including...
A sensitive methodology using antibody-conjugated gold nanoclusters (AuNCs) was developed for the quantitative bioimaging of specific proteins in biological tissues by laser ablation (LA) coupled to inductively plasma–mass spectrometry (ICPMS). Determination metallothioneins (MT1/2 protein isoforms) images human retina tissue sections carried out as a proof concept. AuNCs used label were conjugated selected antibody through carbodiimide coupling. stoichiometry AuNCs/available 1:1 obtained....
We examined the profiling of gene expression metallothioneins (MTs) in human tissues from cadaver eyes with microarray-based analysis. All MT1 isoforms, exception MT1B, were abundantly expressed lens and corneal tissue. Along MT4 was not detected any tissues. Antibodies to MT1/2 labeled epithelial endothelial cells, whereas MT3 label retinal ganglion cells. studied effects zinc cytokines on MT isoforms a cell line (HCEsv). Zinc exerted an up-regulation this effect further potentiated...
Gold nanoclusters (AuNCs) with a diameter of 1.99 nm on average were synthesized and applied as labels in immunoprobes for the determination cytosolic proteins individual human retinal pigment epithelium (HRPEsv) cells by single cell - inductively coupled plasma mass spectrometry (sc-ICP-MS). For quantitative purposes, number gold atoms per immunoprobe (i.e., amplification factor) was determined; 466 obtained. Human metallothioneins (MT), including 2A isoform (MT2A), apolipoprotein E (APOE)...
The persistent lack of adequate matrix-matched reference materials still hinders the quantitative analysis elements and biomolecules in biological samples by LA-ICP-MS. This fact is especially critical cell cultures due to their complex matrix. In this work, we propose a novel calibration strategy, which fully mimics matrix cultured cells, using same line sample create laboratory standards. As model case, imaging two cytosolic proteins (MT2A APOE) individual HRPEsv cells was performed...
LOXL1 gene is the most important genetic risk factor known so far for pseudoexfoliation glaucoma (XFG). Our purpose was to evaluate potential association of individual variants lysyl oxidase-like 1 (LOXL1) and haplotypes with XFG in Spanish patients. Blood samples were collected from a total 105 patients 200 healthy controls. The entire along promoter, coding non-coding regions including 5´- 3´-untranslated regions, sequenced using next-generation sequencing 99 SNPs rs16958477 (promoter),...
We evaluate here the combination of two-dimensional liquid chromatography (2D-LC) in multiple heart cutting mode and isotope dilution tandem mass spectrometry for direct analysis tryptic digests serum samples. As a proof concept, we attempt quantification proteotypic peptides Apolipoprotein AIV (APOA4), Complement C3 (C3) Vitronectin (VTN) which have been previously identified as potential candidate biomarkers glaucoma. Using this 2D-LC strategy, analyte enrichment steps are avoided sample...
Glaucoma is a multifactorial eye disease, characterized by progressive optic neurodegeneration. Elevation of the intraocular pressure main risk factor for glaucoma and consequence an imbalance in aqueous humor hydrodynamics, physiology which influenced homeostatic equilibrium essential elements, oxidative stress, antioxidants. The aim this work was to study local alterations glaucomatous patients from two different, but connected, points view: (i) total antioxidant capacity (as indicator...
The retinal pigment epithelium (RPE) is progressively degenerated during age-related macular degeneration (AMD), one of the leading causes irreversible blindness, which clinical hallmark buildup sub-RPE extracellular material. Clinical observations indicate that Zn dyshomeostasis can initiate detrimental intracellular events in RPE. In this study, we used a primary human fetal RPE cell culture model producing deposits accumulation recapitulates features early AMD to study homeostasis and...
Nutritional supplementation with antioxidants and vitamins is widely recommended in the treatment of vascular disorders affecting retina, although there insufficient evidence on its effectiveness. The vitamin-like compound coenzyme Q10 (CoQ10) a nutritional supplement current interest to treat neurodegenerative diseases. Here, we report retrospective clinical case series study 48 patients diagnosed retinal diseases, including non-arteritic ischemic optic neuropathy (NAION), artery occlusion...
Single cell-inductively coupled plasma-mass spectrometry (sc-ICP-MS) and laser ablation (LA)-ICP-MS have been complementary employed to develop a comprehensive study of APOE claudin-1 expression in ARPE-19 cells submitted glucose treatment (100 mM, 48 h) that induces oxidative stress conditions. Results were compared with control cells. The determination the two proteins by ICP-MS was sequentially carried out using specific immunoprobes labelled IrNCs offer huge amplification (1760 ± 90...
Study of metals and metallothioneins in the human lens by multidisciplinary combination MS bio-analytical methodologies.
To elucidate the potential role of single-nucleotide polymorphisms (SNPs) in complement factor H (CFH) gene Northern Spanish patients with age-related macular degeneration (AMD).A case-control study 130 unrelated native diagnosed AMD (46 dry, 35 neovascular and 49 mixed) 96 healthy controls matched by age ethnicity were enrolled. DNA was isolated from peripheral blood genotyped for AMD-associated SNPs (rs3753394, rs529825, rs800292, rs3766404, rs203674, rs10671170, rs3753396 rs1065489) using...
Antibodies conjugated with naturally abundant and isotopically enriched AgNCs are used for bioimaging of proteins in human retinal layers by LA-ICP-MS.