A5075561078- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Clostridium difficile and Clostridium perfringens research
- Coccidia and coccidiosis research
- Veterinary medicine and infectious diseases
- Gut microbiota and health
- Pharmacological Effects of Natural Compounds
- RNA Research and Splicing
- Liver Diseases and Immunity
- Cutaneous lymphoproliferative disorders research
- RNA and protein synthesis mechanisms
- Animal Nutrition and Physiology
- Gene Regulatory Network Analysis
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Antimicrobial Resistance in Staphylococcus
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Helminth infection and control
- Nanopore and Nanochannel Transport Studies
- Telomeres, Telomerase, and Senescence
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mast cells and histamine
Duke University
2022-2025
Experimental Pathology Laboratories
2024
North Carolina State University
2021-2023
Duke Cancer Institute
2023
Duke Medical Center
2023
Cancer Institute (WIA)
2023
North Central State College
2021
Cincinnati Children's Hospital Medical Center
2009-2019
Clostridium perfringens-induced necrotic enteritis (NE) is an economically important disease of broiler chickens. The present study evaluated the effect C. perfringens on intestinal histomorphometry, enteric microbial colonization, and host immune responses using 3 experimental NE reproduction methods. groups consisted 1) unchallenged Control diet (corn-soybean meal), 2) + Eimera inoculation at d 11 followed by challenge 15 (ECp), 3) Wheat-based (WCp), 4) Eimeria (WECp). results showed that...
Erythroderma is a severe and heterogeneous inflammatory skin condition with little guidance on the approach to management in cases of unknown etiology. To guide therapeutic selection, we sought create an immunophenotyping platform able identify aberrant cell populations cytokines subtypes erythroderma. We performed high-parameter flow cytometry peripheral blood mononuclear cells (PBMCs) whole patient refractory idiopathic erythroderma, erythrodermic patients Sézary syndrome pityriasis rubra...
Complement, NKT, and NK cells play critical roles in the first line defense against pathogens. Functional for both C5a receptors, that is, complement receptor (C5aR) receptor-like 2 (C5L2), sepsis have been demonstrated. However, role of innate lymphocyte activation during remains elusive. In this article, we show naive NKT already express high levels C5aR minor C5L2 mRNA, but no protein. Upon Escherichia coli-induced sepsis, found surface expression on subpopulations cells, suggesting rapid...
Respuestas inmunes celulares en tejidos linfoides de pollos engorde infectados experimentalmente con cepas Clostridium perfringens productoras enteritis necrótica. Las respuestas del huésped contra (CP), el agente causante la necrótica (NE) pollos, son poco conocidas. En presente estudio, primero se analizó capacidad producción siete C. netB+ (CP5, CP18, CP26, CP64, CP67, CP68 y NCNE-1), utilizando un modelo infección experimental engorde. La evaluación las lesiones macroscópicas...
Environmental and genetic factors define the susceptibility of an individual to autoimmune disease. Although common pathways affect general immunological tolerance mechanisms in autoimmunity, effects such genes could vary under distinct immune challenges within different tissues. In this study, we demonstrate by observing that type 1 diabetes-protective haplotypes at insulin-dependent diabetes region 10 (Idd10) introgressed from chromosome 3 C57BL/6 (B6) A/J mice onto NOD background increase...
We have previously proposed that sequence variation of the CD101 gene between NOD and C57BL/6 mice accounts for protection from type 1 diabetes (T1D) provided by insulin-dependent susceptibility region 10 (Idd10), a <1 Mb on mouse chromosome 3. In this study, we provide further support hypothesis Cd101 is Idd10 using haplotype expression analyses novel congenic strains coupled to development knockout mouse. Susceptibility T1D was correlated with genotype-dependent multiple cell subsets,...
The present study evaluated the avian macrophage responses against Clostridium perfringens that varied in their ability to cause necrotic enteritis chickens. Strains CP5 (avirulent-netB+), CP1 (virulent-netB+), and CP26 (highly virulent-netB+tpeL+) were used evaluate effect on macrophages (MQ-NCSU cells) primary splenic cecal tonsil mononuclear cells. bacilli (whole or secretory products from all three strains induced a significant increase transcription of Toll-like receptor (TLR)21, TLR2,...
Control of the electrochemical environment in living cells is typically attributed to ion channels. Here we show that formation biomolecular condensates can modulate cells, which affects processes globally within cell and interactions with its environment. Condensate results depletion or enrichment certain ions, generating intracellular gradients. These gradients directly affect properties a cell, including cytoplasmic pH hyperpolarization membrane potential. The modulation equilibria...
Despite having long been postulated, compelling evidence for the theory that microbial triggers drive autoimmunity has only recently reported. A specific association between Novosphingobium aromaticivorans, an ubiquitous alphaproteobacterium, and primary biliary cirrhosis (PBC) uncovered in patients with PBC. Notably, infection PBC confirmed a mouse model which leads to development of liver lesions resembling concomitant production anti-PDC-E2 antibodies cross-react conserved PDC-E2 epitopes...
Type 1 diabetes (T1D) is a chronic multi-factorial disorder characterized by the immune-mediated destruction of insulin-producing pancreatic beta cells. Variations at large number genes influence susceptibility to spontaneous autoimmune T1D in non-obese diabetic (NOD) mice, one most frequently studied animal models for human disease. The genetic analysis these mice allowed identification many insulin-dependent (Idd) loci and candidate genes, them being Cd101. CD101 heavily glycosylated...
Atopic dogs often are managed with allergen-specific immunotherapy (AIT) and concurrent dosages of ciclosporin (CSA) or oclacitinib to alleviate their clinical signs. Both drugs might affect proper tolerance induction by inhibiting regulatory T-cell (Treg) induction.We evaluated Treg cell numbers serum interleukin (IL)-10 transforming growth factor-beta (TGF-β)1 levels in diagnosed atopic dermatitis (AD) successfully treated either CSA for nine more months.We included 15 receiving...
Herpesvirus of turkey (HVT) increases activation T cells in 1-day-old chickens when administered
Enumeration of Eimeria oocysts is a common practice in monitoring coccidiosis turkeys; however, the conventional method manual microscopic examination time-consuming. Previously, we used flow cytometry (FCM) to enumerate and speciate coccidia affecting chickens here, extended those findings turkey species, E. adenoides meleagrimitis. Using FCM, commercial vaccine containing these species was optimize scatter-plot parameters, including Forward-(size) Side-(shape/granularity) scatter Area,...
Abstract Doxorubicin treatment induces DNA damage and apoptosis in rapidly dividing cell types like intestinal epithelial cells. This has been demonstrated both vivo vitro . In certain some cells do not undergo damage-induced response to doxorubicin but instead become senescent. Induction of senescence these can lead dysfunction chronic inflammation, which more damage. We questioned whether a single dose would be able induce For studies, we exposed IEC-6 small evaluate is induced relatively...
Background: Erythroderma is characterized by diffuse redness and inflammation involving a majority of the skin. The pathophysiology erythroderma heterogeneous, with many cases unknown etiology variable treatment response. For in which underlying not well understood, there little clinical guidance on approach. We thus sought to create an immunophenotyping platform able identify differential biomarkers various subtypes guide selection targeted therapeutics. Methods: report index case...