A5061695528- Retinal Development and Disorders
- Intracranial Aneurysms: Treatment and Complications
- Neurobiology and Insect Physiology Research
- Cellular Mechanics and Interactions
- melanin and skin pigmentation
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Neurosurgical Procedures and Complications
- Moyamoya disease diagnosis and treatment
- Spinal Dysraphism and Malformations
- Pluripotent Stem Cells Research
- Cerebrospinal fluid and hydrocephalus
- Vascular Malformations Diagnosis and Treatment
- Animal Genetics and Reproduction
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Meningioma and schwannoma management
- Near-Field Optical Microscopy
- Traumatic Brain Injury Research
- S100 Proteins and Annexins
- Ocular Oncology and Treatments
- Biochemical Analysis and Sensing Techniques
- Ear and Head Tumors
Hamamatsu University School of Medicine
2021-2024
Marine Biological Laboratory
2001-2024
Nagoya University
2013-2024
Almeida Memorial Hospital
2013-2022
Oita University
1999-2017
St. Marianna University School of Medicine
2017
National Institute of Oceanography and Fisheries
2015
Nagatomi Neurosurgical Hospital
2013
FuelCell Energy (United States)
2013
Kyoto University
2000-2011
We found adult human stem cells that can generate, from a single cell, with the characteristics of three germ layers. The are stress-tolerant and be isolated cultured skin fibroblasts or bone marrow stromal cells, directly aspirates. These self-renew; form characteristic cell clusters in suspension culture express set genes associated pluripotency; differentiate into endodermal, ectodermal, mesodermal both vitro vivo. When transplanted immunodeficient mice by local i.v. injection, integrated...
The stochastic and elite models have been proposed for the mechanism of induced pluripotent stem (iPS) cell generation. In this study we report a system that supports model. We previously identified multilineage-differentiating stress-enduring (Muse) cells in human dermal fibroblasts are characterized by stress tolerance, expression pluripotency markers, self-renewal, ability to differentiate into endodermal-, mesodermal-, ectodermal-lineage from single cell. They can be isolated as...
We report here the striking anisotropy of fluorescence exhibited by crystals native green protein (GFP). The were generated water dialysis highly purified GFP obtained from jellyfish Aequorea . find that becomes six times brighter when excitation, or emission, beam is polarized parallel (compared with perpendicular) to crystal long axis. Thus, major dipoles fluorophores must be oriented very nearly Observed in a polarizing microscope between polars instead either polarizer analyzer alone,...
Dendritic chromatophores that contained blue pigmentary organelles were found in the bluish parts of skin two callionymid species, mandarin fish, Synchiropus splendidus, and psychedelic S. picturatus. We named these novel cells “cyanophores” “cyanosomes”. In response to various stimulatory cues, cyanophores responded by aggregation or dispersion cyanosomes. addition their role revelation hues, may participate changes shades gorgeous fish.
Object. The authors investigated whether revascularization surgeries in children with moyamoya disease could reduce abnormal collateral formations the posterior circulation; long-term follow-up angiography was performed to assess status. Methods. In six patients who underwent indirect bypass surgery childhood, studies were obtained between 7 and 12 years after surgery. All presented ischemic symptoms at onset of 2 age. Ischemic insults did not occur any patient. five cases, angiographically...
Summary In the reddish‐violet parts of skin diadema pseudochromis Pseudochromis , we found novel dichromatic chromatophores with a reddish pigment and reflecting platelets. We named these cells ‘erythro‐iridophores’. standard physiological solution, erythro‐iridophores displayed two hues, red dark violet when viewed an optical microscope under ordinary transmission light epi‐illumination optics, respectively. Under electron microscopy, however, observed no typical chromatosomes, i.e.,...
Organelles in cells are appropriately positioned, despite crowding the cytoplasm. However, our understanding of force required to move large organelles, such as nucleus, inside cytoplasm is limited, part owing a lack accurate methods for measurement. We devised novel method apply forces nucleus living, wild-type Caenorhabditis elegans embryos measure generated cell. utilized centrifuge polarizing microscope (CPM) centrifugal and orientation-independent differential interference contrast...
Measurements of spectral reflectance from the sky-blue portion skin common surgeonfish, Paracanthurus hepatus, showed a relatively steep peak at around 490 nm. We consider that multilayer thin-film interference phenomenon non-ideal type, which occurs in stacks very thin light-reflecting platelets iridophores region, is primarily responsible for revelation hue. The structural organization iridophore closely resembles bluish damselfish species, although one difference presence monolayer...
The wavelength of the light reflected from iridophores depends on thickness and spacing intracellular reflecting platelets. Here, we show that rapid color change blue to red chameleon sand tilefish Hoplolatilus chlupatyi is mediated by adrenergic stimulation a novel type iridophore in which platelets are concentrated selectively periphery cell, near plasma membrane. changes not only observed vivo but also pigment cells isolated scales respond increases K+ ion concentrations 0.5 s addition...
To understand the pathogenesis of diffuse axonal injury, we investigated temporal and spatial profiles neuronal degeneration in impact-acceleration injury rats using Fluoro-Jade (FJ) staining. Impact-acceleration was produced Wistar by method described Marmarou et al. with some modifications. Animals were sacrificed 1, 2, 7, 14, or 28 days after injury. Paraffin-embedded coronal sections stained HE FJ, analyzed immunohistochemically for GFAP amyloid precursor protein (APP). FJ-positive...
Organelles in cells are appropriately positioned, despite crowding the cytoplasm. However, our understanding of force required to move large organelles, such as nucleus, inside cytoplasm is limited, part owing a lack accurate methods for measurement. We devised method apply forces nucleus living Caenorhabditis elegans embryos measure generated cell. used centrifuge polarizing microscope centrifugal and orientation-independent differential interference contrast microscopy characterize mass...
The general background hue of the skin domino damsel (Dascyllus trimaculatus; Pomacentridae) is velvet-black, but three white spots, one on median parietal region and other two around base dorsal fin, form a characteristic, conspicuous pattern for this species. In dark region, dermal melanophores dense monolayer, while in thick accumulation non-dendritic iridophores present dermis, which lined with layer melanophores. velvet-black area extend their processes parallel to plane skin, respond...
Biological aspects between cultivated and wild sea bass, Dicentrarchus labrax were evaluated. Fish samples collected seasonally from Boughaz El-Maadiya in the eastern Mediterranean coast fish farm. The length-weight relationship of seabass two habitats calculated condition factor for captured region (Mean ± S.d = 1.044 0.0905) was lower than that farm 1.158 0.8119). mean value natural mortality coefficient 0.172 0.017. Significant seasonal difference degrees stomach fullness (F 3.589, df 3,...
To determine the exact role of TGF-α in glial activation after traumatic brain injury, we investigated astroglial and microglial responses cortical stab wound injury overexpressing mice. Adult male B6D2-TgN (MMTVTGFA) 29RjC transgenic mice were used for subjects. This line carries a cDNA under control dexamethasone-inducible MMTV promoter. Thus, exogenous administration dexamethasone induces overexpression. Male B6D2F1/J at same age served as wild-type animals. After expression fibrilary...