A5064051900- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Telomeres, Telomerase, and Senescence
- Occupational and environmental lung diseases
- Neonatal Respiratory Health Research
- Mesenchymal stem cell research
- Lung Cancer Treatments and Mutations
- IL-33, ST2, and ILC Pathways
- Occupational exposure and asthma
- Immune cells in cancer
- PI3K/AKT/mTOR signaling in cancer
- Medical Imaging and Pathology Studies
- Epigenetics and DNA Methylation
- Hepatitis B Virus Studies
- Cervical Cancer and HPV Research
- Lung Cancer Research Studies
- Systemic Sclerosis and Related Diseases
- Eosinophilic Disorders and Syndromes
- Pleural and Pulmonary Diseases
- Immune Response and Inflammation
- Oral and Maxillofacial Pathology
- Cancer-related gene regulation
- Virus-based gene therapy research
- Eosinophilic Esophagitis
- Cell Adhesion Molecules Research
- Tumors and Oncological Cases
University of Michigan
2014-2023
Southwest Jiaotong University
2019
Ann Arbor Center for Independent Living
2017
Michigan United
2014-2015
Matrix Research (United States)
2015
Xi'an Jiaotong University
2002
Xi'an University of Technology
2000
The origin of fibroblasts in pulmonary fibrosis is assumed to be intrapulmonary, but their extrapulmonary and especially derivation from bone marrow (BM) progenitor cells has not been ruled out. To examine this possibility directly, adult mice were durably engrafted with BM isolated transgenic expressing enhanced GFP. Induction such chimera by endotracheal bleomycin (BLM) injection caused large numbers GFP(+) appear active fibrotic lesions, while only a few could identified control lungs....
The origin of fibroblasts in pulmonary fibrosis is assumed to be intrapulmonary, but their extrapulmonary and especially derivation from bone marrow (BM) progenitor cells has not been ruled out. To examine this possibility directly, adult mice were durably engrafted with BM isolated transgenic expressing enhanced GFP. Induction such chimera by endotracheal bleomycin (BLM) injection caused large numbers GFP+ appear active fibrotic lesions, while only a few could identified control lungs....
Abstract Increased lung IL-4 expression in pulmonary fibrosis suggests a potential pathogenetic role for this cytokine. To dissect role, bleomycin-induced inflammation and were analyzed compared wild type (IL-4+/+) vs IL-4-deficient (IL-4−/−) mice. Lethal injury after bleomycin treatment was higher IL-4−/− IL-4+/+ By administration of anti-CD3 Abs, we demonstrated that early response linked to the marked T lymphocyte infiltration overproduction proinflammatory mediators such as TNF-α, IFN-γ,...
Abstract Found in inflammatory zone (FIZZ)1, also known as resistin-like molecule α, belongs to a novel class of cysteine-rich secreted protein family, named FIZZ/resistin-like molecule, with unique tissue expression patterns. FIZZ1 is induced alveolar type II epithelial cells (AECs) bleomycin (BLM)-induced lung fibrosis, and found induce myofibroblast differentiation vitro. The objective this study was elucidate the regulation AEC pulmonary fibrosis. AECs were isolated from rat lungs...
In addition to its expression in stem cells and many cancers, telomerase activity is transiently induced murine bleomycin (BLM)-induced pulmonary fibrosis with increased levels of transcriptase (TERT) expression, which essential for fibrosis. To extend these observations human chronic fibrotic lung disease, we investigated the fibroblasts from patients interstitial diseases (ILDs), including idiopathic (IPF). The results showed that was more than 66% IPF fibroblast samples, comparison less...
The Wnt/β-catenin pathway initiates a signaling cascade that is critical in cell differentiation and the normal development of multiple organ systems. reactivation this has been documented experimental human idiopathic pulmonary fibrosis, wherein activation implicated epithelial-cell repair. Furthermore, canonical ligand Wnt3a known to induce myofibroblast differentiation; however, role noncanonical Wnt ligands remains unclear. This study showed significantly higher levels Wnt11 expression...
Abstract Leukocyte infiltration is characteristic of lung injury and fibrosis, its role during tissue repair fibrosis incompletely understood. We found that overexpression IL-5 in transgenic mice (IL-5TG) or by adenoviral gene transfer increased bleomycin (blm)-induced injury, eosinophilia. Surprisingly, blm-treated IL-5-deficient (IL-5−/−) also developed pronounced pulmonary but characterized marked T lymphocyte absence In both murine strains however, induction TGF-β expression was evident....
In addition to its well-known expression in the germline and cells of certain cancers, telomerase activity is induced lung fibrosis, although role this process unknown. To identify pathogenetic importance we examined effects reverse transcriptase (TERT) deficiency a murine model pulmonary injury. TERT-deficient mice showed significantly reduced fibrosis following bleomycin (BLM) insult. This was accompanied by significant reduction α-SMA, marker myofibroblast differentiation. Furthermore,...
FIZZ (found in inflammatory zone) 1, a member of cysteine-rich secreted protein family, is highly induced lung allergic inflammation and bleomycin fibrosis, primarily expressed by airway type II alveolar epithelial cells. This novel mediator known to stimulate α-smooth muscle actin collagen expression fibroblasts. The objective this study was investigate the vivo effects FIZZ1 on development fibrosis evaluating bleomycin-induced pulmonary deficient mice. knockout mice exhibited no detectable...
Hedgehog signaling plays important roles in cell development and differentiation. In this study, the ability of Sonic (SHH) to induce myofibroblast differentiation was analyzed isolated human lung fibroblasts, its vivo significance evaluated rodent bleomycin-induced pulmonary fibrosis. The results showed that SHH could fibroblasts a Smo- Gli1-dependent manner. Gel shift analysis, chromatin immunoprecipitation assay, site-directed mutagenesis revealed Gli1 binding consensus α-SMA gene...
Abstract Amphiregulin (AREG), an epidermal growth factor receptor ligand, is implicated in tissue repair and fibrosis, but its cellular source role regeneration versus fibrosis remain unclear. In this study, we hypothesize that AREG induced bone marrow–derived CD11c+ cells essential for pulmonary fibrosis. Thus, the objectives were to evaluate importance of identify induction, analyze regulation fibroblast function activation. The results showed lung expression was significantly...
Found in inflammatory zone (FIZZ) 2, also known as resistin-like molecule (RELM)-β, belongs to a novel cysteine-rich secreted protein family named FIZZ/RELM. Its function is unclear, but closely related member, FIZZ1, has profibrotic activities. The human ortholog of rodent FIZZ1 not been identified, FIZZ2 significant sequence homology both (59%) and (50%). Given the greater FIZZ2, analyzing role model bleomycin-induced pulmonary fibrosis would be potential relevance fibrotic lung disease....
Mutations in the genes encoding telomerase reverse transcriptase (TERT) and telomerase's RNA components as well shortened telomeres are risk factors for idiopathic pulmonary fibrosis, where repetitive injury to alveolar epithelium is considered a key factor pathogenesis. Given importance of TERT stem cells, we hypothesized that plays an important role epithelial repair its deficiency results exacerbation fibrosis by impairing this repair/regenerative process. To evaluate generated type II...
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Gut-enriched Krüppel-like factor (GKLF) has been reported to partially inhibit alpha-smooth muscle actin (alpha-SMA) gene transcription by competing for binding the TGF-beta control element (TCE) with known activators such as Sp1 and other factors. This incomplete inhibition via TCE suggests an additional mechanism, which was evaluated in this study. The results showed that alpha-SMA promoter mutated remained susceptible GKLF rat lung fibroblasts consistent existence of TCE-independent...
Stem cell factor (SCF) and its receptor c-Kit have been implicated in tissue remodelling fibrosis. Alveolar fibroblasts from patients with diffuse interstitial fibrosis secrete more SCF. However, precise role remains unclear. In this study the potential of SCF-c-Kit axis pulmonary was examined. Fibrosis induced by intratracheal instillation bleomycin (BLM), which caused increased SCF levels plasma, bronchoalveolar lavage fluid (BALF) lung tissue, as well expression fibroblasts. These changes...
Recent evidence supports that bone marrow (BM)-derived hematopoietic progenitor cells play an important role in lung injury and fibrosis. While these give rise to multiple cell types, the ST2 (Il1rl1)-expressing group 2 innate lymphoid (ILC2s) derived from BM progenitors have been implicated tissue repair remodeling, including To further investigate precise of BM-derived ILC2s pathogenesis fibrotic disease, their importance bleomycin-induced fibrosis model was evaluated by analyzing effects...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES