A5082134361- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolic Engineering and Bioproduction
- Lipid metabolism and biosynthesis
- Phytochemical compounds biological activities
- Catalysis for Biomass Conversion
- Amino Acid Enzymes and Metabolism
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Dental Health and Care Utilization
- Oral microbiology and periodontitis research
- Genetic Mapping and Diversity in Plants and Animals
- Enzyme Catalysis and Immobilization
- Fungal Biology and Applications
- Enzyme-mediated dye degradation
- Dysphagia Assessment and Management
- Bioenergy crop production and management
- Biopolymer Synthesis and Applications
- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- HIV/AIDS oral health manifestations
- Autophagy in Disease and Therapy
- Polysaccharides and Plant Cell Walls
- Microbial Fuel Cells and Bioremediation
Hiroshima University
2021-2024
National Institute for Materials Science
2022
National Institute of Advanced Industrial Science and Technology
2017-2020
Food Research Institute
2009-2014
Food Research Institute
2009-2013
Tokai University
2005-2011
University of Tsukuba
2007
Shimizu (Japan)
2005
Rice University
2002
Institute of Agrobiological Sciences
2002
The rice species Oryza sativa is considered to be a model plant because of its small genome size, extensive genetic map, relative ease transformation and synteny with other cereal crops1,2,3,4. Here we report the essentially complete sequence chromosome 1, longest in genome. We summarize characteristics structure biological insight gained from sequence. analysis 43.3 megabases (Mb) non-overlapping reveals 6,756 protein coding genes, which 3,161 show homology proteins Arabidopsis thaliana,...
To determine the chromosomal positions of expressed rice genes, we have performed an sequence tag (EST) mapping project by polymerase chain reaction–based yeast artificial chromosome (YAC) screening. Specific primers designed from 6713 unique EST sequences derived 19 cDNA libraries were screened on 4387 YAC clones and used for map construction in combination with genetic analysis. Here, describe establishment a comprehensive YAC-based transcript that contains 6591 sites covers 80.8% genome....
Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide hemicellulose fraction of lignocellulosic biomass. For effective biomass utilization, it is to understand mechanism substrate recognition by these enzymes. Recent studies have shown that specificities bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different. While functional differences been described, still...
Exo-1,5-α-L-arabinofuranosidases belonging to glycoside hydrolase family 43 have strict substrate specificity. These enzymes hydrolyze only the α-1,5-linkages of linear arabinan and arabino-oligosaccharides in an exo-acting manner. The enzyme from Streptomyces avermitilis contains a core catalytic domain C-terminal binding module carbohydrate 42. We determined crystal structure intact exo-1,5-α-L-arabinofuranosidase. is composed 5-bladed β-propeller topologically identical other enzymes. had...
The possibility of using two kinds sorghum as raw materials in consolidated bioprocessing bioethanol production Flammulina velutipes was investigated. Enzymatic saccharification sweet not high brown mid-rib (bmr) mutated sorghum, but the amount ethanol higher. Ethanol from bmr significantly increased when enzymes were added to culture.
Basidiomycetes have the ability to degrade lignocellulosic biomass, and some basidiomycetes produce alcohol dehydrogenase. These characteristics may be useful in direct production of ethanol from lignocellulose. Ethanol fermentation by was investigated examine possibility consolidated bioprocessing (CBP) using Flammulina velutipes. F. velutipes converted d-glucose with a high efficiency (a theoretical recovery rate 88%), but pentose not observed. properties are similar those Saccharomyces...
Plasmid pL32 from the Natto strain of Bacillus subtilis belongs to a group low-copy-number plasmids in gram-positive bacteria that replicate via theta mechanism replication. We studied DNA region encoding replication protein, RepN, pLS32, and obtained following results. Transcription repN gene starts 167 nucleotides upstream translational start site repN. The copy number repN-coding plasmid pHDCS2, which was placed downstream IPTG (isopropyl-1-thio-beta-D-galactopyranoside)-inducible Pspac...
Abstract This study aimed to clarify the association of daily physical activity and leisure-time exercise with risk dysphagia in community-dwelling Japanese older adults using a questionnaire-based survey. We analyzed 3070 participants (1657 men, 1413 women; age 66 ± 4 years [mean SD]) Shizuoka Daiko studies within Multi-Institutional Collaborative Cohort study. used Dysphagia Risk Assessment for Community-dwelling Elderly questionnaire assess International Physical Activity Questionnaire...
To develop a gene transformation method for Flammulina velutipes, we constructed vector with hph under control of the trp1 promoter. The was integrated into protoplast derived from mycelia by calcium-polyethylene glycol method, as it has not been reported F. velutipes. Transformation efficiency much improved when performed restriction enzyme mediated integration method.
Bacterial extracellular vesicles (BEVs), produced by a broad spectrum of bacteria, play crucial role in enhancing intercellular communication through DNA transfer. A vital determinant their gene transfer efficiency is the content diversity within BEVs, an aspect that conventional metagenomics fails to capture. Our study bridges this gap with novel microdroplet-based sequencing technique precisely details hundreds individual BEVs. This revealed unique profile BEVs from oral pathogen...
Background: The aim of this prospective cohort study was to clarify changes in physical and oral function older adults after completing a 3-month health program combining exercise, instruction, nutritional guidance. Methods: Subjects were 34 women aged at least 70 years (mean age 79.2 years) Bungotakada City, Oita Prefecture, Japan. Physical investigated on the first day (baseline), end program, 6 12 months program. measured using handgrip strength test, timed up go (TUG) test one-leg...
The effect of lime pretreatment brown midrib sorghums on enzymatic saccharification was investigated. Under most the conditions, yields bmrs were higher than those normal counterparts. This result suggests that bmr is useful to reduce costs, because amount necessary for biomass can reduced by using mutants.
A GH67 α-glucuronidase gene derived from Bacillus halodurans C-125 was expressed in E. coli to obtain a recombinant enzyme (BhGlcA67). Using the purified enzyme, enzymatic properties and substrate specificities of were investigated. BhGlcA67 showed maximum activity at pH 5.4 45 °C. When incubated with birchwood, oat spelts, cotton seed xylan, did not release any glucuronic acid or 4-O-methyl-glucuronic these substrates. acted only on...
To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control target genes. When hygromycin B phosphotransferase (hph) from Escherichia coli was controlled by gpd promoter, transformation efficiency 3-fold higher than case that tryptophan synthetase (trp1) promoter.
Polyethylene glycol (PEG)-induced cell fusion is a promising method to transfer larger DNA from one another than conventional genetic systems. The laboratory strain Bacillus subtilis 168 contains restriction (R) and modification (M) system, BsuM, which recognizes the sequence 5'-CTCGAG-3'. To study whether BsuM system affects by PEG-induced between R(+)M(+) R(-)M(-) strains, we examined of plasmids pHV33 pLS32neo carrying no eight sites, respectively. It was shown that although but not cells...
Dental caries and periodontal disease are typical oral diseases frequently observed in patients with renal diseases. Tooth loss is an outcome of dental disease, the number existing teeth indicator health status. However, association between end-stage kidney (ESKD) has not been investigated detail. This study aimed to investigate status, expressed by teeth, ESKD. We analyzed data from second survey Longitudinal Evaluation Multi-phasic, Odontological, Nutritional Associations Dentists, a...
Abstract Aim The involvement of human papillomavirus (HPV) E6/E7 in autophagy has not been fully elucidated oral squamous cell carcinoma (OSCC) cells. objective this study was to clarify the association between HPV and cancer stem properties such as self‐renewal ability chemotherapy resistance HPV16‐positive OSCC Methods UM‐SCC‐104 cells were cultured on laminin 332‐coated silicone gel. impact HPV16 E6 or E7 siRNA transfection proliferation, spheroid formation, 5‐fluorouracil (5‐FU)‐induced...