A5056374760- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- DNA and Nucleic Acid Chemistry
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Advanced Glycation End Products research
- Biochemical and Molecular Research
- Microbial Metabolites in Food Biotechnology
- Supramolecular Self-Assembly in Materials
- Catalytic Cross-Coupling Reactions
- Crystallization and Solubility Studies
- Catalytic C–H Functionalization Methods
- Biofuel production and bioconversion
- HIV/AIDS drug development and treatment
- Diet, Metabolism, and Disease
- RNA and protein synthesis mechanisms
- X-ray Diffraction in Crystallography
- Polydiacetylene-based materials and applications
- Polysaccharides and Plant Cell Walls
- Amino Acid Enzymes and Metabolism
- Biochemical effects in animals
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
Gifu University
2015-2024
Tokushima University
2024
Kayaba Industry (Japan)
2022
Tokyo Seiei College
2017
Harvard University
2015-2016
Food Research Institute
1990-2016
National Agriculture and Food Research Organization
2013-2016
Gifu Pharmaceutical University
2007-2014
National Institutes of Health
2010
SPring-8
2009
A mild and efficient ligand-free Suzuki-Miyaura coupling reaction catalyzed by heterogeneous Pd/C was developed. Aryl bromides triflates undergo the cross-coupling with aryl boronic acids in excellent yields without presence of any additives aqueous media at room temperature. vinyl are also applicable to this provide trans-stilbene derivatives high yields. The application wet-type achieved loss activity under aerobic conditions, reuse is feasible for a fifth run significant activity....
We have developed a mild and efficient protocol for the ligand-free heterogeneous Pd/C-catalyzed hetero Suzuki–Miyaura coupling reaction that allows synthesis of both heteroaryl–aryl heteroaryl–heteroaryl derivatives in good to excellent yields.
Abstract A ligand‐free and heterogeneous palladium on carbon (Pd/C)‐catalyzed hetero‐Suzuki–Miyaura coupling reaction has been developed. The protocol enables the construction of both heterocyclic‐alicyclic heterocyclic‐heterocyclic biaryl derivatives in good to excellent yields. Furthermore, Pd/C could be reused. time‐course study clarified that was leached into media as proceeded then completely deposited support.
Abstract Various biofunctional hydrogel materials can be fabricated in aqueous media through the self-assembly of peptide derivatives, forming supramolecular nanostructures and their three-dimensional networks. In this study, we describe new Fmoc-dipeptides comprising α-methyl-L-phenylalanine. We found that position number methyl groups introduced onto α carbons by α-methyl-L-phenylalanine have a marked influence on morphology nanostructure as well (network) formation ability.
Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide hemicellulose fraction of lignocellulosic biomass. For effective biomass utilization, it is to understand mechanism substrate recognition by these enzymes. Recent studies have shown that specificities bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different. While functional differences been described, still...
The 2'-5'-oligoadenylate synthetase (OAS)/RNase L pathway is an innate immune system that protects hosts against pathogenic viruses and bacteria through cleavage of exogenous single-stranded RNA; however, this system's selective targeting mechanism remains unclear. Here, we identified mRNA quality control factor Dom34 as a novel restriction for positive-sense RNA virus. Downregulation RNase increases viral replication, well half-life the RNA. directly binds to form surveillance complex...
Copper catalysts supported on chelate resins bearing iminodiacetate moieties (DIAION CR11) or polyamine CR20) as chelating functional groups (12% Cu/CR11 and 7% Cu/CR20, respectively) were developed. 12% effectively catalyzed the Huisgen cycloaddition of mono-substituted alkynes to azides in presence triethylamine under totally solvent-free conditions afford corresponding 1,4-disubstituted 1,2,3-triazoles excellent yields a completely regioselective manner. Furthermore, was found proceed...
d-Alanine-d-alanine ligase (Ddl) is one of the key enzymes in peptidoglycan biosynthesis and an important target for drug discovery. The enzyme catalyzes condensation two d-Ala molecules using ATP to produce d-Ala-d-Ala, which terminal peptide a monomer. structures five forms from Thermus thermophilus HB8 (TtDdl) were determined: unliganded TtDdl (2.3 Å resolution), TtDdl–adenylyl imidodiphosphate (2.6 Å), TtDdl–ADP (2.2 TtDdl–ADP–d-Ala (1.9 Å) TtDdl–ATP–d-Ala-d-Ala Å). central domain...
SUMMARY Plasmodium falciparum thymidylate kinase (PfTMK) can tolerate a range of substrates, which distinguishes it from other kinases. The enzyme not only phosphorylates TMP and dUMP but also bulkier purines, namely, dGMP, GMP, dIMP. In order to probe the flexibility PfTMK in accommodating ligands various sizes, we developed 6 mutant enzymes subjected these thermodynamic, inhibitory catalytic evaluation. Kinase activity was markedly affected by introducing larger lysine residue instead...
Artificial supramolecular nanostructures showing transient properties have attracted significant attention in recent years. New discoveries this area may provide insights into a better understanding of the sophisticated organization complex biomolecular systems. Nevertheless, research concerning such materials is still limited. Better knowledge chemical reactivity and corresponding molecular transformations self-assembling molecules, which guide their assembly/disassembly, an opportunity to...
A simple synthesis of sugar fatty acid esters was developed in a nonaqueous solution using lipase modified by synthetic detergent. Esterification accelerated continuous removal water from the reaction mixture with molecular sieve. When glucose and palmitic (1:1 mole) were used as starting substrates, more than 90% converted to its ester this system. The resultant product 6-O-palmitoylglucose. Other mono- or disaccharides also esterified high yield. It shown that might act catalyst for esters.
The metabolism of levoglucosan (1,6-anhydro-β-d-glucopyranose) in several microorganisms was studied. Although could be easily hydrolyzed to glucose with acid, all the tested yeasts and fungi strains had no activity hydrolyzing it. On other hand, formation 6-phosphate from presence Mg-ATP2− observed reaction cell extracts levoglucosan-assimilating fungi. enzyme catalyzing this shown independent general hexokinases by an ion-exchange, chromatography or polyacrylamide gel electrophoresis...
Aqueous hybrid soft nanomaterials consisting of plural supramolecular architectures with a high degree segregation (orthogonal coexistence) and precise hierarchy at the nano- microscales, which are reminiscent complex biomolecular systems, have attracted increasing attention. Remarkable progress has been witnessed in construction DNA nanostructures obtained by rational sequence design peptide derivatives through self-assembly under aqueous conditions. However, orthogonal single medium not...
A polymer-supported copper catalyst (Cu/HP20) is easily prepared in water and effectively catalyzed the Huisgen cycloaddition between azides terminal alkynes.
Gene silencing and RNA interference are major cellular processes that control gene expression via the cleavage of target mRNA. Eukaryotic translation initiation factor 2C2 (EIF2C2, Argonaute protein 2, Ago2) is considered to be player RNAi as it core component RISC complexes. While a considerable amount research has focused on its associated mechanisms, nature mechanisms nucleotide recognition by PAZ domain EIF2C2/Ago2 have not yet been characterized. Here, we demonstrate an inherent lack...
A levoglucosan (1,6-anhydro-beta-D-glucopyranose)-using bacterium, isolated from soil, was identified. It shown to belong the genus Arthrobacter and tentatively named sp. I-552. novel enzyme catalyzed dehydrogenation of form 1,6-anhydro-beta-D-ribo-hexopyranos-3-ulose (3-keto levoglucosan), using NAD+ as an electron acceptor, i.e. NAD+: 1,6-anhydro-beta-D-glucopyranose oxidoreductase (trivial name: dehydrogenase). This purified characterized. possible reaction scheme for glucose formation...
The metabolism of levoglucosan (l, 6-anhydro-β-D-glucopyranose) in several microorganisms was studied. Although could be easily hydrolyzed to glucose with acid, all the tested yeasts and fungi strains had no activity hydrolyzing it. On other hand, formation 6-phosphate from presence Mg-ATP2- observed reaction cell extracts levoglucosan-assimilating fungi. enzyme catalyzing this shown independent general hexokinases by an ion-exchange chromatography or poly aery lamide gel electrophoresis...
Abstract A practical and efficient preparation method of palladium‐fibroin (Pd/Fib), silk‐fibroin‐supported Pd(0) by means sonication, has been developed. The Pd/Fib catalyst could be prepared within 12 h at room temperature starting from commercial silk‐fibroin Pd(OAc)2 in MeOH, whereas our previous required least 4 days. present improved process is applicable to a large‐scale Pd/Fib. the also catalyzed chemoselective hydrogenation acetylenes, olefins, azides presence aromatic ketones,...