A5008477050- Phytochemicals and Antioxidant Activities
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Postharvest Quality and Shelf Life Management
- Botanical Research and Applications
- X-ray Diffraction in Crystallography
- Fermentation and Sensory Analysis
- Crystallization and Solubility Studies
- Contact Dermatitis and Allergies
- Dye analysis and toxicity
- Polysaccharides and Plant Cell Walls
- Proteins in Food Systems
- Plant Gene Expression Analysis
- Enzyme Production and Characterization
- Biochemical Analysis and Sensing Techniques
- Protein Hydrolysis and Bioactive Peptides
- Bone Tissue Engineering Materials
- Natural Antidiabetic Agents Studies
- Protein Interaction Studies and Fluorescence Analysis
- Polyamine Metabolism and Applications
- Chemical synthesis and alkaloids
- Plant biochemistry and biosynthesis
- Food composition and properties
- Synthetic Organic Chemistry Methods
- Garlic and Onion Studies
Universität Hamburg
2023-2025
University of Stuttgart
2016-2025
Hamburg Institut (Germany)
2024
Stuttgart Observatory
2019-2022
National Research Tomsk State University
2017
Tomsk Polytechnic University
2017
Norwich Research Park
2016
Quadram Institute
2016
University of Hohenheim
2011-2013
Zero to Three
2012
To understand the bioaccessibility of flavonoid quercetin we studied its interaction with bile salt micelles. The environmental sensitivity quercetin's UV-visible absorption spectrum gave information about partitioning. Two peaks complementary information: Peak A (240-280nm) on intermicellar phase and B (340-440nm) micellar phase. Thus, by altering pH, showed that only non-ionised partitions into We validated our interpretation studying SDS Pyrene fluorescence spectra show adsorption site...
We have studied the uptake of quercetin aglycone into CaCo-2/TC7 cells in presence and absence mixed micelles that are present human small intestine. The inhibited transport cells. To gain an understanding why this is case we examined solubilisation differing composition pure lipid phases. did by using environmental sensitivity quercetin's UV-visible absorption spectra measurement free filtration micellar solutions. nature was also pyrene fluorescence. found partitioning simple bile salt low...
Phenolic structures are of great interest due to their antioxidant properties and various postulated benefits on human health. However, the quantification these in fruits vegetables, as well vivo or vitro experiments, is demanding, relevant concentrations often low, causing problems exactly weighing respective amounts. Nevertheless, determination used a prerequisite for accurate results. A possibility quantify polyphenol use UV/vis spectroscopy. Therefore, absorption coefficients selected...
The FT‐Raman spectra of the chloride salts pelargonidin‐3‐glucoside, cyanidin‐3‐glucoside and delphinidin‐3‐glucoside, their structures at pH 5.0 interaction with aluminium ferric ions are presented for first time discussed regard to spectroscopic response. Two marker bands approximately 1510 1330/1350 cm −1 one band were identified being characteristic formation chelates, respectively, delphinidin‐3‐glucoside. In contrast, exhibiting single hydroxyl group in B‐ring, did not form metal which...
A blood glucose level lowering effect is postulated for polyphenols (PPs), which in part attributed to the inhibition of α-amylase. To estimate structure-effect relationships, chlorogenic acid (CA), phlorizin (PHL), epigallocatechin gallate (EGCG), epicatechin (EC), and malvidin-3-glucoside (Mlv-3-glc) were used as inhibitors an enzyme assay, on basis conversion GalG2CNP by The detection CNP was performed UV/vis spectroscopy. data reveal that inhibitor strength decreases follows: EGCG >...
Health benefits of apple polyphenols for different chronic diseases are postulated. To exert bioactive properties, absorption into the body is required (bioavailability), which strongly influenced by matrix release (bioaccessibility). For seven varieties, in vitro experiments with simulated saliva fluid (SSF) and ex vivo digestion centrifuged human were conducted. Polyphenol characterization (high-performance liquid chromatography-tandem mass spectrometry) quantification (high performance...
For flavan-3-ols, significant effects to prevent the development of diabetes mellitus are postulated. Inter alia, this is attributed inhibitory on intestinal α-amylase, in particular for high-molecular-weight procyanidins. In order gain a deeper insight into mode interaction and resulting α-amylase inhibition, between monomers (+)-catechin (CAT) (-)-epicatechin (EC), dimers procyanidin (PC) B1 PC B2, trimer C1 their inhibition porcine pancreatic were investigated. Weak interactions...
Cylindramide (1) was built up from three components: a hydroxyornithine derivative 7, tetrazolylsulfone 8, and substituted pentalene subunit 9. Derivative 7 prepared in six-step reaction sequence involving the Wittig Sharpless asymmetric dihydroxylation starting N-Boc-3-aminopropanal (12). Tetrazolylsulfone 8 accessible four steps dioxinone 22. The synthesis of fragment 9 started cycloocta-1,5-diene 26, that converted into enantiopure bicyclo[3.3.0]octanedione 29. latter functionalized to...
Interactions between phenolic compounds and the allergen Mal d 1 are discussed to be reason for better tolerance of apple cultivars, which rich in polyphenols. Because is susceptible proteolytic digestion allergenic symptoms usually restricted mouth throat area, release native during oral phase particular interest. Therefore, we studied under different vitro conditions revealed that only 6–15% total present apples released. To investigate proposed polyphenol-Mal interactions, various...
Patients who suffer from birch pollinosis often develop adverse reactions to the consumption of fresh apples due structural similarity allergens Bet v 1 and Mal d apples, respectively. A different allergenic potential for isoallergens is postulated, but approaches quantify isoallergen-specific are missing. Therefore, a bottom-up proteomics approach was developed by stable isotope dilution microHPLC-QTOF analyses. Marker peptides individual (Mal 1.01-Mal 1.03 1.06), combinations thereof 1.01...
The apple allergy in Northern Europe is a cross-reaction to the birch pollen allergy. No correlation between allergenicity of an variety and content major allergen Mal d 1, homologue Bet v 1 birch, could be found using ELISA, so far. Therefore, impact polyphenols and/or differences isoallergen profile are discussed. To allow more detailed analysis profile, mass spectrometric method was applied investigate flesh peel 10 traditional varieties commercial breeds. data revealed often, but not...
Abstract Contamination with Cd, Cu and Pb is a major environmental issue. Cations of those metals may adsorb on negatively charged surfaces polymeric silicic acid (pSi), altering their fate. Aiming to elucidate the underlying mechanisms extent adsorption, we conducted batch adsorption experiments at pH 4 6 concentrations that excluded precipitation solid phases. Zeta-potential measurements were monitor surface charge changes. In addition, isothermal titration calorimetry (ITC) was used...