A5009182800- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Immune cells in cancer
- T-cell and B-cell Immunology
- Mast cells and histamine
- Cancer, Hypoxia, and Metabolism
- Systemic Lupus Erythematosus Research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- HIV Research and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
- Respiratory viral infections research
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- Pneumonia and Respiratory Infections
- Neonatal Respiratory Health Research
- Synthesis of β-Lactam Compounds
- Neuroscience of respiration and sleep
- CAR-T cell therapy research
- Pediatric health and respiratory diseases
- Climate Change and Health Impacts
- Diet and metabolism studies
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Atherosclerosis and Cardiovascular Diseases
Jackson Laboratory
2018-2024
Tufts University
2020-2024
University of Maine
2020-2024
Jackson Laboratories (India)
2024
University of California, San Francisco
2024
Kaohsiung Chang Gung Memorial Hospital
2018-2023
Chang Gung Memorial Hospital
2017-2020
Memorial Hospital of South Bend
2020
Chang Gung University
2014-2019
Washington University in St. Louis
2013-2018
A characteristic of memory T (T M ) cells is their ability to mount faster and stronger responses reinfection than naïve N do in response an initial infection. However, the mechanisms that allow this rapid recall are not completely understood. We found CD8 have more mitochondrial mass and, upon activation, resulting secondary effector E proliferate quickly, produce cytokines, maintain greater ATP levels primary cells. also after increase oxidative phosphorylation aerobic glycolysis sustain a...
Competition for nutrients like glucose can metabolically restrict T cells and contribute to their hyporesponsiveness during cancer. Metabolic adaptation the surrounding microenvironment is therefore key maintaining appropriate cell function. For instance, cancer use acetate as a substrate alternative fuel metabolism growth. Here, we show that rescues effector function in glucose-restricted CD8+ cells. Mechanistically, promotes histone acetylation chromatin accessibility enhances IFN-γ gene...
Abstract This unit contains several protocols to determine the energy utilization of T cells in real‐time using a Seahorse Extracellular Flux Analyzer ( http://www.seahorsebio.com ). The advantages this machine over traditional metabolic assays include simultaneous measurement glycolysis and mitochondrial respiration, real‐time, on relatively small numbers cells, without any radioactivity. Basic Protocol describes standard stress test XF e 96, which yields information about oxidative...
Neuroblastoma is a common childhood tumor and accounts for 15% of pediatric cancer deaths. To investigate the microRNA (miRNA) profile role Dicer Drosha in neuroblastoma, we assessed expression 162 human miRNAs, 66 neuroblastoma tumors by using real-time PCR methods. We found global downregulation miRNA advanced identified 27 miRNAs that can clearly distinguish low- from high-risk patients. Furthermore, levels or were low tumors, which accounted disease correlated with poor outcome. Notably,...
The rapid evolution of Human Immunodeficiency Virus (HIV-1) allows studies ongoing host–pathogen interactions. One key selective host factor is APOBEC3G (hA3G) that can cause extensive and inactivating Guanosine-to-Adenosine (G-to-A) mutation on HIV plus-strand DNA (termed hypermutation). inhibit this innate anti-viral defense through binding the viral protein Vif to hA3G, but efficiency varies hypermutation frequencies fluctuate in patients. A pivotal question whether hA3G-induced G-to-A...
The major cancer related mortality is caused by metastasis and invasion. It important to identify genes regulating invasion in order curtail metastatic spread of cells. This study investigated the association between RUNX2 miR-10a/miR-10b risk breast relapse. Expression levels miR-10a/b in108 pairs tumor non-tumor tissue were assayed quantitative PCR analysis evaluated for their prognostic implications. median expression miR-10b normalized using adjacent significantly higher relapsed...
Arginine is a semiessential amino acid in healthy adult human, but essential for preterm, newborn or critically ill patients. can be supplied from our diet de novo synthesis citrulline. In conditions of sepsis endotoxemia, arginine may deficient and accompanied with altered immune response. L-arginine supplementation ameliorate dysregulated condition improve prognosis. Many studies had tried L-citrulline to examine the effect on response population. Few studied young children. this study, we...
Mycoplasma pneumoniae is one of the major pathogens causing community-acquired pneumonia in children. Although usually self-limited, (MPP) may lead to complicated morbidity that can even be life-threatening. Upon MPP infection, alveolar macrophage becomes attracted and activated will induce subsequent cytokine chemokine reaction. Refractory (RMPP) manifested by clinical or radiological deterioration despite proper antibiotic therapy. RMPP characterized with excessive inflammation need...
All children experience upper respiratory tract illnesses (URI) caused by viral infections. However, some of these progress to the lower airways. Although studies have found infection with certain species are more likely trigger (LRIs), a comprehensive analysis viruses underlying early-life LRIs is lacking. Determine incidence URIs, mild and severe (mLRI, sLRI) during first 2 years life association between pathogens odds versus URIs in Puerto Rican children, population at high risk for...
Craniofacial structure is an important determinant of obstructive sleep apnoea (OSA) syndrome risk. Three-dimensional stereo-photogrammetry (3dMD) a novel technique which allows quantification the craniofacial profile. This study compares facial images OSA patients captured by 3dMD to three-dimensional computed tomography (3-D CT) and two-dimensional (2-D) digital photogrammetry. Measurements were correlated with indices severity.Thirty-eight diagnosed included, photogrammetry, 3-D CT...
In the real world, dynamic changes in air pollutants and meteorological factors coexist simultaneously. Studies identifying effects of individual on acute exacerbation (AE) asthma may overlook health overall combination. A comprehensive study examining influence pollution is required. Asthma AE data from emergency room visits were collected Taiwan National Health Insurance Research Database. Complete monitoring for (SO2; NO2; O3; CO; PM2.5; PM10) Environmental Protection Agency stations....
Tumor levels of the cell cycle regulators cyclin E and p27 correlate strongly with survival in breast cancer patients are specifically regulated by ubiquitin ligases hCDC4 SKP2. This study was to explore whether genetic susceptibility is associated polymorphism these genes gene-gene gene-risk factor [i.e. full-term pregnancy (FTP)] interactions important determining risk. A two-stage case-control based on single-nucleotide polymorphisms performed. The first (560 cases 1122 controls) define...
Aberrant metabolic demand is observed in immune/inflammatory disorders, yet the role pathogenesis remains unclear. Here, we discover that lupus, activated B cells, including germinal center (GCB) have remarkably high glycolytic requirement for survival over T cell populations, as demonstrated by increased activity lupus-activated cells compared to immunization-induced cells. The augmented reliance on glucose oxidation makes GCB vulnerable mitochondrial ROS-induced oxidative stress and...