A5100613656- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Adenosine and Purinergic Signaling
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA Research and Splicing
- Lipid metabolism and disorders
- Nonmelanoma Skin Cancer Studies
- Genomics, phytochemicals, and oxidative stress
- Plant and Fungal Interactions Research
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Occupational and environmental lung diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Folate and B Vitamins Research
- CNS Lymphoma Diagnosis and Treatment
- Asthma and respiratory diseases
- Ferroptosis and cancer prognosis
- Pregnancy and Medication Impact
- Cancer Genomics and Diagnostics
Ruian People's Hospital
2018-2024
Wenzhou Medical University
2018-2024
Guangdong Medical College
2010-2022
Affiliated Hospital of Guangdong Medical College Hospital
2022
Guangdong General Hospital
2015-2020
Guangdong Academy of Medical Sciences
2017-2020
Ningbo University
2020
Sun Yat-sen University
2015-2019
Huazhong University of Science and Technology
2015-2019
Guangdong Provincial People's Hospital
2019
The human genome encodes many long non-coding RNAs (lncRNAs). However, their biological functions, molecular mechanisms, and the prognostic value associated with pancreatic ductal adenocarcinoma (PDAC) remain to be elucidated. Here, we identify a fundamental role for lncRNA HOXA transcript at distal tip (HOTTIP) in progression chemoresistance of PDAC.High-throughput microarrays were performed detect expression profiles lncRNAs messenger eight PDAC tissues four tissues. Quantitative real-time...
Abstract Background Colorectal carcinoma (CRC) is one of the leading causes cancer-related mortality worldwide. MicroRNAs (miRNAs, miRs) play important roles in carcinogenesis. MiR-32 has been shown to be upregulated CRC. In this study, we identified potential effects miR-32 on some biological properties CRC cells, and clarified regulation PTEN by miR-32. Methods The effect expression was assessed cell lines with mimics/inhibitor increase/decrease expression. Furthermore, regulating cells...
Background MicroRNAs (miRNAs) are important post-transcriptional regulators. Altered expression of miRNAs has recently demonstrated association with human ulcerative colitis (UC). In this study, we attempted to elucidate the roles miR-126 in pathogenesis UC. Methods Expression miR-126, miR-21, miR-375 and potential targets NF-κB inhibitor alpha (IκBα, IKBA or NFKBIA), Polo-like kinase 2 (PLK2) v-Crk sarcoma virus CT10 oncogene homolog (CRK) were assessed 52 colonic biopsies from patients...
Pancreatic ductal adenocarcinoma (PDAC) is still a lethal malignancy. Long noncoding RNAs (lncRNAs) have been shown to play critical role in cancer development and progression. Here we identified overexpression of the lncRNA AFAP1-AS1 PDAC patients evaluated its prognostic functional relevance. The global expression profile was measured by microarray. Expression reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR) 90 tissue samples adjacent normal tissues. impact on cell...
// Yingxue Wang 1, * , Zhihua Li 2, Shangyou Zheng Yu Zhou 1 Lei Zhao 3 Huilin Ye Xiaohui 4 Wenchao Gao Zhiqiang Fu Quanbo Yimin Liu Rufu Chen Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department Hepatopancreatobiliary Surgery, Sun Yat-sen Memorial Hospital, University, Guangzhou, China 510120 2 Medical Oncology, Radiation Yat-Sen University Cancer Center, 510060 These authors have contributed equally to this work Correspondence to: Chen, e-mail:...
Abstract Melanoma contributes a lot to skin cancer‐related deaths. lncRNAs are implicated in various diseases, including melanoma. lncRNA NEAT1 is frequently dysregulated and can play important roles multiple cancers. Nevertheless, little has been studied about the function of melanoma progression. In our present research, we displayed was overexpressed cells. A series functional assays showed that overexpression promoted proliferation, migration, invasion By contrast, knockdown obviously...
Glutamate dehydrogenase (GDH) is a key enzyme that catalyzes the final reaction of glutamine metabolic pathway, and has been reported implicated in tumor growth metastasis. However, it’s clinical significance role colorectal cancer (CRC) pathogenesis largely unknown. The expression GDH was determined by qPCR, western blot immunohistochemistry CRC cells samples. correlation with clinicopathologic features prognosis analyzed. functional cell proliferation, motility metastasis evaluated. We...
Pancreatic ductal adenocarcinoma (PDAC) cells utilize a novel non-canonical pathway of glutamine metabolism that is essential for tumor growth and redox balance. Inhibition this metabolic in PDAC can potentially synergize with therapies increase intracellular reactive oxygen species (ROS) such as radiation. Here, we evaluated the dependence pancreatic cancer stem (PCSCs) on researched whether inhibiting enhance radiosensitivity PCSCs. We showed deprivation significantly inhibited...
Recent evidence shows that altered microRNA-126 (miR-126) expression is implicated in the progression of colorectal cancer (CRC). However, precise roles and mechanisms miR-126 CRC remain unclear. The aim this study was to investigate cells elucidate miR-126-mediated cells. First, analyzed using qRT-PCR 4 human cell lines (SW480, SW620, HT-29 HCT-116). Furthermore, biological properties vitro were examined by applying Cell Counting Kit 8, cycle, apoptosis transwell assays. pathways detected...
Background Colorectal carcinoma (CRC) is one of the leading causes cancer-related mortality worldwide. MicroRNAs (miRNAs, miRs) play important roles in carcinogenesis. MiR-126 has been shown to be down-regulated CRC. In this study, we identified potential effects miR-126 on some biological properties CRC cells and clarified regulation insulin receptor substrate 1 (IRS-1) its possible signaling pathway by miR-126. Methods The effect IRS-1, AKT, ERK1/2 expression was assessed cell lines HT-29...
Abstract MicroRNAs (miRNAs) act as important post-transcriptional regulators of gene expression by targeting the 3′-untranslated region their target genes. Altered miR-16 is reported in human ulcerative colitis (UC), but its role development disease remains unclear. Adenosine through adenosine A2a receptor (A2aAR) could inhibit nuclear factor-kappaB (NF-κB) signaling pathway inflammation. Here we identified overexpression and down-regulation A2aAR colonic mucosa active UC patients. We...
MicroRNA‐126 (miR‐126) has been reported to be a tumor suppressor that targets CXCR4 in colorectal cancer (CRC) cells. This study investigated whether miR‐126 any prognostic impact patients with CRC. MiR‐126 and mRNA expression 92 pairs of CRC adjacent nontumorous tissues was examined using quantitative real‐time PCR, protein assessed by immunohistochemistry (IHC) Western blotting. The correlation between clinicopathological features overall survival rate determined. downregulated expressed...
Abstract The role of the adenosine A3 receptor (A3AR) in experimental colitis is controversial. A3AR agonist N 6 -(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) has been shown to have a clinical benefit, although studies A3AR-deficient mice suggest pro-inflammatory role. However, there are no on effect 2-Cl-IB-MECA and molecular mechanism action murine models vivo. Is it same as that observed vitro? interaction between 2-CL-IB-MECA model signaling pathways associated with this remain...
Introduction The aim of present study was to profile the glucose-dependent and glutamine- dependent metabolism in pancreatic cancer. Methods We performed Immunohistochemical staining GLUT1, CAIX, BNIP3, p62, LC3, GLUD1, GOT1. Based on expression metabolism-related proteins, metabolic phenotypes tumors were classified into two categories, including glucose- glutamine-dependent metabolism. There Warburg type, reverse mixed null type metabolism, canonical non-canonical Results Longer overall...
Interleukin (IL)-23 and IL-12 are closely related in structure, these cytokines regulate both innate adaptive immunity. However, the precise signaling networks that production of each Toxoplasma gondii-infected THP-1 monocytic cells, particularly PI3K/AKT MAPK pathways, remain unknown. In present study, T. gondii infection upregulated expression IL-23 increased with parasite dose. secretion was strongly inhibited by TLR2 monoclonal antibody (mAb) treatment a dose-dependent manner siRNA...
Curcumin (Cur), derived from Curcuma species, exhibits anti-inflammatory, antioxidant, and anticancer effects. Although Cur has some beneficial effects on asthma, its clinical application is limited by low bioavailability. Tetrahydrocurcumin (THC), the major active metabolite of Cur, multiple biological functions, similarly to importantly, it showed enhanced bioavailability in tissues plasma. However, effect THC asthma not been reported.The current study sought investigate efficacy dietary...
Cell adhesion molecule 1 (CADM1/TSLC1), a putative tumor suppressor involving in cell adhesion, proliferation and apoptosis, is frequently inactivated several carcinomas due to promoter hypermethylation. But alterations of CADM1/TSLC1 colorectal carcinogenesis clinical significance have not been elucidated yet. The aim this study was determine the role functional inactivation gene tumorigenesis its potential as novel epigenetic marker for assessment patients with cancer. We measured...
MicroRNAs (miRNAs, miRs) are suspected to play important roles in carcinogenesis. MiR‐32 has altered expression colorectal cancer (CRC); however, the clinical significance of miR‐32 process carcinogenesis is poorly understood. In this study, we determined levels of, correlation between, and phosphatase tensin homologue ( PTEN ), a tumor suppressor targeted by miR‐32, CRC. The gene 35 carcinoma samples, corresponding cancer‐adjacent tissue 27 adenoma 16 normal samples were quantified using...
Cutaneous squamous cell carcinoma (cSCC) is a malignancy of epidermal keratinocytes that responsible for ~20% annual skin cancer-associated mortalities. Accumulating evidence demonstrates the dysregulation micro (mi)RNAs serves significant role in tumorigenesis and progression human cSCC. MicroRNA-31 (miR-31) upregulated cSCC involved development. However, underlying mechanism remains unclear. The present study demonstrated miR-31 line, A-431, expression contributes to proliferation invasion...
Restenosis after angioplasty or stent is a major clinical problem. While long noncoding RNAs (lncRNAs) are implicated in variety of diseases, their role restenosis not well understood. This study aims to investigate how dysregulated lncRNAs and messenger (mRNAs) contribute restenosis. By microarray analysis, we identified 202 625 mRNAs (fold change > 2.0, p < 0.05) differentially expressed between the balloon-injured carotid artery uninjured rats. Among lncRNAs, LncRNA CRNDE had highest fold...
Long non-coding RNA (lncRNA) is a variety of the human transcriptome that does not code for proteins and plays an important role in development progression multiple solid malignant tumors. However, roles lncRNAs pancreatic ductal adenocarcinoma (PDAC) remain unknown. In this study, we investigated expression patterns three PDAC tumor samples (T) relative to those matched adjacent non-tumor tissues (N) via microarray with 30,586 lncRNA probes 26,109 mRNA probes. The revealed 27,279 samples,...
Colon cancer is one of the major causes cancer-related deaths worldwide. Long non-coding RNA (lncRNA) LINC01123 has been suggested to act as an oncogene in non-small cell lung and a prognostic signature head neck squamous carcinoma. However, its role colon remains obscure. From TCGA database, was observed be up-regulated adenocarcinoma (COAD). Subsequently, also detected cells. Functionally, could enhance proliferation, migration, invasion angiogenesis. Moreover, chemoresistance cells...