MicroRNAs (miRNAs), i.e. small non-coding RNA molecules (∼22 nt), can bind to one or more target sites on a gene transcript negatively regulate protein expression, subsequently controlling many cellular mechanisms. A current and curated collection of miRNA–target interactions (MTIs) with experimental support is essential thoroughly elucidating miRNA functions under different conditions in species. As database, miRTarBase has accumulated than 3500 MTIs by manually surveying pertinent...

Abstract Background Colorectal carcinoma (CRC) is one of the leading causes cancer-related mortality worldwide. MicroRNAs (miRNAs, miRs) play important roles in carcinogenesis. MiR-32 has been shown to be upregulated CRC. In this study, we identified potential effects miR-32 on some biological properties CRC cells, and clarified regulation PTEN by miR-32. Methods The effect expression was assessed cell lines with mimics/inhibitor increase/decrease expression. Furthermore, regulating cells...

Background MicroRNAs (miRNAs) are important post-transcriptional regulators. Altered expression of miRNAs has recently demonstrated association with human ulcerative colitis (UC). In this study, we attempted to elucidate the roles miR-126 in pathogenesis UC. Methods Expression miR-126, miR-21, miR-375 and potential targets NF-κB inhibitor alpha (IκBα, IKBA or NFKBIA), Polo-like kinase 2 (PLK2) v-Crk sarcoma virus CT10 oncogene homolog (CRK) were assessed 52 colonic biopsies from patients...

The aim of this study was to investigate the efficacy exenatide and insulin glargine in patients with newly diagnosed type 2 diabetes mellitus (T2DM) nonalcoholic fatty liver disease (NAFLD).We performed a 24-week randomized controlled multicentre clinical trial. Seventy-six were randomly assigned 1:1 receive or treatment. endpoints included changes fat content (LFC), visceral adipose tissue (VAT), subcutaneous (SAT) measured by magnetic resonance spectroscopy, blood glucose, enzymes, lipid...

Abstract MicroRNAs (miRNAs) act as important post-transcriptional regulators of gene expression by targeting the 3′-untranslated region their target genes. Altered miR-16 is reported in human ulcerative colitis (UC), but its role development disease remains unclear. Adenosine through adenosine A2a receptor (A2aAR) could inhibit nuclear factor-kappaB (NF-κB) signaling pathway inflammation. Here we identified overexpression and down-regulation A2aAR colonic mucosa active UC patients. We...

Abstract The role of the adenosine A3 receptor (A3AR) in experimental colitis is controversial. A3AR agonist N 6 -(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) has been shown to have a clinical benefit, although studies A3AR-deficient mice suggest pro-inflammatory role. However, there are no on effect 2-Cl-IB-MECA and molecular mechanism action murine models vivo. Is it same as that observed vitro? interaction between 2-CL-IB-MECA model signaling pathways associated with this remain...

Increasing evidence suggests that miRNAs are widely dysregulated in ulcerative colitis (UC), potentially affecting UC pathogenesis, diagnosis, and therapy. microRNA (miR) -206 has been reported to be upregulated UC; however, its function role remain unknown. Here, we elucidate the of miR-206 pathogenesis UC. In patients with active-UC, adenosine A3 receptor (A3AR) levels were significantly downregulated, respectively, inversely correlated. A3AR was expressed colon mucosa (particularly...

Abstract Background Pioglitazone is a promising therapeutic method for nonalcoholic fatty liver disease (NAFLD) patients with or without type 2 diabetes. However, there remarkable variability in treatment response. We analyzed our previous randomized controlled trial to examine the effects of gender and other factors on efficacy pioglitazone treating Chinese abnormal glucose metabolism. Methods This post hoc analysis randomized, parallel controlled, open-label clinical (RCT) an original...

MicroRNAs (miRNAs, miRs) are suspected to play important roles in carcinogenesis. MiR‐32 has altered expression colorectal cancer (CRC); however, the clinical significance of miR‐32 process carcinogenesis is poorly understood. In this study, we determined levels of, correlation between, and phosphatase tensin homologue ( PTEN ), a tumor suppressor targeted by miR‐32, CRC. The gene 35 carcinoma samples, corresponding cancer‐adjacent tissue 27 adenoma 16 normal samples were quantified using...

To investigate the expression of adenosine A3 receptor (A3AR) in human colonic epithelial cells and effects A3AR activation on tumor necrosis factor alpha (TNF- α -) induced inflammation order to determine its mechanism action cells, (HT-29 cells) were treated with different concentrations 2-Cl-IB-MECA prior TNF- stimulation, followed by analysis NF- κ B signaling pathway downstream IL-8 IL-1 β production. mRNA protein expressed HT-29 not altered changes or 2-Cl-IB-MECA. Pretreatment...

Colon cancer is one of the major causes cancer-related deaths worldwide. Long non-coding RNA (lncRNA) LINC01123 has been suggested to act as an oncogene in non-small cell lung and a prognostic signature head neck squamous carcinoma. However, its role colon remains obscure. From TCGA database, was observed be up-regulated adenocarcinoma (COAD). Subsequently, also detected cells. Functionally, could enhance proliferation, migration, invasion angiogenesis. Moreover, chemoresistance cells...

Toxoplasma gondii is a parasite that primarily infects through the oral route. Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) play crucial roles in immune responses generated during parasitic infection and also drive inflammatory response against invading parasites. However, little known about regulation of NLRs inflammasome activation T. gondii-infected human small intestinal epithelial (FHs 74 Int) cells. FHs Int cells infected with were subsequently evaluated for...

Abstract Background A recently proposed diagnostic criteria of metabolic dysfunction-associated fatty liver disease (MAFLD) is more available for various clinical situations than nonalcoholic (NAFLD), but understanding about differences between NAFLD and MAFLD in practice remains limited the general adult urban population China. Methods total 795 subjects were recruited from Wu Song Branch Zhongshan Hospital who participated health assessment. Examination results was obtained through...

Abstract Colorectal cancer (CRC) is a common gastrointestinal malignancy. Long noncoding RNAs (lncRNAs) are associated with the progression of various cancers, including CRC. Herein, we explored function lncRNA LINC01550 in expression CRC was analyzed using The Cancer Genome Atlas (TCGA). diagnostic value evaluated ROC curves. relationship between clinicopathological variables and explored, its prognostic assessed Kaplan–Meier Cox regression analyses. immune cell infiltration CIBERSORT....

Aim: To investigate the anticancer mechanisms of silver nanoparticles (AgNPs) in colorectal cancer. Methods: Anticancer effects AgNPs were determined cancer HCT116 cells and xenograft mice using cellular molecular methods. Results: induced mitochondrial reactive oxygen species production, dysfunction endoplasmic reticulum (ER) stress responses through NOX4 led to cell apoptosis. Pretreatment with DPI or 4-PBA significantly inhibited apoptosis, ER response, expression AgNP-treated cells. also...

The association between serum Retinol Binding Protein 4 (RBP4) and obesity is still controversial. Serum RBP4 levels varies by gender, estradiol may play a role in the difference. To investigate participation of sex hormones humans, we measured RBP4, BMI, 87 women from outpatient. Eighty-seven subjects Chinese origin outpatient (aged 40.22 ± 15.54 years) were enrolled. Subjects with diseases affecting metabolic state or not suitable to participate this study excluded. Anthropometrics...

Increased microRNA (miR)‑32 expression in colorectal cancer (CRC) tissues enhances CRC cell proliferation, migration, invasion and attenuates apoptosis by repressing the of phosphatase tensin homolog (PTEN). Forkhead box K1 (FOXK1) was identified as a potential interacting transcription factor using DNA pull‑down assays mass spectrometry. The present study aimed to elucidate role FOXK1 regulating miR‑32 CRC. expressions <em>FOXK1</em>, miR‑32, transmembrane protein 245 gene...

Colorectal cancer (CRC) is a common human malignant tumor, and the fourth most cause of cancer‑associated mortality in China. However, pathogenesis CRC not yet fully understood. The present study aimed to investigate expression clinical significance microRNA (miR)‑126 insulin receptor substrate‑1 (IRS‑1), as well role miR‑126 prognosis patients with CRC. A total 86 colorectal tissue specimens, including 40 adjacent normal tissue, 26 adenoma 20 samples, were collected for study. Reverse...

The pathogenesis of colorectal cancer (CRC) is poorly understood. MicroRNA (miR)-32 upregulation in CRC tissues was previously reported, where it increased the proliferation, migration and invasion, reduced apoptosis cells by inhibiting expression phosphatase tensin homolog (PTEN). However, mechanism underlying miR-32 remains unknown. an intronic miRNA located within intron 14 transmembrane protein 245 gene (TMEM245). present study aimed to elucidate biological pathways regulation CRC. A...

Toxoplasma gondii is an apicomplexan zoonotic protozoan parasite that infects most species of warm-blooded animals, including humans. The heavy incidence and severe or lethal damage caused by T. infection clearly indicate a need for the development effective vaccine. GRA8 member dense granules protein family used as marker acute infection. In present study, we evaluated protective immunity induced DNA vaccination based on recombinant eukaryotic plasmid, pDsRed2-GRA8, against toxoplasmosis in...

This study presents a novel approach to analysing environmental governance by Chinese governments through the lens of tripartite evolutionary game model. The key novelty our research lies in explicit incorporation reputation mechanism into analysis, which significantly influences government decision-making, strategies, and dynamics relationship between polluting enterprises. By assessing marginal effects conventional mobilization-based on mechanism, as well collusion behaviours enterprises,...

MicroRNA-32 (miR-32) is upregulated in colorectal cancer (CRC) tissues; its overexpression leads to increased cell proliferation, migration and invasion, as well reduced apoptosis of CRC cells, at least partly by inhibiting the target gene phosphatase tensin homolog. However, mechanisms upregulation have remained elusive. In present study, effects methylation acetylation on expression miR-32 were investigated. The promoter status lines HT-29 HCT-116 normal colonic epithelial line NCM460 was...