A5000252148- Renal Diseases and Glomerulopathies
- Gut microbiota and health
- Renal and related cancers
- Clostridium difficile and Clostridium perfringens research
- Genetic and Kidney Cyst Diseases
- Biomedical Research and Pathophysiology
- Microscopic Colitis
- Neonatal Health and Biochemistry
- Celiac Disease Research and Management
- Vasculitis and related conditions
- Complement system in diseases
- Topic Modeling
- Gastrointestinal motility and disorders
- Pediatric Urology and Nephrology Studies
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Reinforcement Learning in Robotics
- Adolescent and Pediatric Healthcare
- Neuroinflammation and Neurodegeneration Mechanisms
- Myasthenia Gravis and Thymoma
- Eosinophilic Esophagitis
- Acute Kidney Injury Research
- Health Policy Implementation Science
- Computational and Text Analysis Methods
- Forecasting Techniques and Applications
- IgG4-Related and Inflammatory Diseases
Shanghai Children's Hospital
2013-2023
Shanghai Jiao Tong University
2013-2023
University of Pennsylvania
2023
Weatherford College
2020
Shanghai Ninth People's Hospital
2018
Harvard University
2016
Brigham and Women's Hospital
2015-2016
Hong Kong Polytechnic University
2013-2014
Glomerular diseases are commonly characterized by podocyte injury including apoptosis, actin cytoskeleton rearrangement and detachment. However, the strategies for preventing damage remain insufficient. Recently autophagy has been regarded as a vital cytoprotective mechanism keeping homeostasis. Thus, it is reasonable to utilize this attenuate injury. Trehalose, natural disaccharide, an mTOR independent inducer. It unclear whether trehalose alleviates Therefore, we investigated efficacy of...
Abstract Background Primary nephrotic syndrome (PNS) is a common glomerular disease in children. T cell dysfunction plays crucial role the pathogenesis of PNS. Moreover, dysbiosis gut microbiota contributes to immunological disorders. Whether initial therapy PNS affects remains an important question. Our study investigated compositional changes after therapy. Methods Fecal samples 20 children with were collected before and 4-week Total bacteria DNA extracted V3-V4 regions 16S ribosomal RNA...
Prednisone is widely used in chronic glomerular diseases, immunological disorders, and rheumatic diseases for its anti-inflammatory immunosuppressive properties. It a synthetic glucocorticoid (GC) that shows therapeutic effects after conversion to prednisolone by the liver.
Hemolytic uremic syndrome caused by Shiga toxin–producing Escherichia coli (STEC HUS) is a worldwide endemic problem, and its pathophysiology not fully elucidated. Here we tested whether the mannose-binding lectin (MBL2), an initiating factor of complement pathway activation, plays crucial role in STEC HUS. Using novel human MBL2-expressing mice (MBL2 KI) that lack murine Mbls (MBL2+/+Mbl1–/–Mbl2–/–), HUS model consisted intraperitoneal injection with toxin-2 (Stx-2) or without anti-MBL2...
Vancomycin (VCM) has long been used clinically to fight against Gram-positive bacterial infections. In recent decades, an increased number of kidney injury cases caused by VCM overdose have reported. this study, we further investigated the mechanism VCM-overdose-induced injury.Immunohistochemistry (IHC) staining, RT-qPCR and Western blot assays were determine ki67, DDX5, PTGS2, GPX4 SLC7A11 expressions in tissues mice. CCK-8 flow cytometry HK2 cell viability apoptosis. addition, was applied...
Hemophagocytic lymphohistiocytosis, which includes primary (familial) and secondary hemophagocytic is a fatal disease in children. Macrophage activation syndrome was defined patients who met lymphohistiocytosis criteria with an underlying autoimmune disease. High-volume hemofiltration has shown beneficial effects severe sepsis multiple organ dysfunction syndrome. Secondary lymphohistiocytosis/macrophage shares many pathophysiologic similarities sepsis. The present study assessed the of...
In this work we introduce reinforcement learning techniques for solving lexicographic multi-objective problems. These are problems that involve multiple reward signals, and where the goal is to learn a policy maximises first signal, subject constraint also second so on. We present family of both action-value gradient algorithms can be used solve such problems, prove they converge policies lexicographically optimal. evaluate scalability performance these empirically, demonstrating their...
Autophagy is a ubiquitous catabolic process involving degradation of damaged organelles and protein aggregates. It shows cytoprotective effects in many cell types helps to maintain homeostasis. In glomerular diseases, podocyte damage leads the disruption renal filtration barrier subsequent proteinuria. Puromycin aminonucleoside (PAN) which induces apoptosis vitro vivo widely used for studying pathophysiology diseases. has been shown that PAN autophagy podocytes. However, relationship between...
Temporal Interaction Graphs (TIGs) are widely utilized to represent real-world systems. To facilitate representation learning on TIGs, researchers have proposed a series of TIG models. However, these models still facing two tough gaps between the pre-training and downstream predictions in their ``pre-train, predict'' training paradigm. First, temporal discrepancy inference data severely undermines models' applicability distant future dynamically evolving data. Second, semantic divergence...
To investigate the expression of response gene to complement 32 (RGC32) in rat with acute kidney injury (AKI) and explore role RGC32 renal repair induced by ischemia reperfusion. Rats were randomly divided into two groups, including sham operation group (n = 48) reperfusion (IRI) 48). sacrificed following 2 h, 6 24 48 72 1 week (w), w, 4 w. The distribution tissue observed means immunohistochemistry. mean density images detected Image-Pro Plus was designated as representative levels....
Children with primary nephrotic syndrome (PNS) who relapse after glucocorticoid therapy are shown to have a decreased total proportion of butyrate-producing bacteria in the gut at onset. Glucocorticoid treatment changes microbiota composition. It is unclear whether remission right and other than associated PNS relapse.PNS paediatric patients within 1 year was recorded. The composition, profiled 16S rRNA gene V3-V4 region sequencing, compared between relapsing non-relapsing children onset...
Glucocorticoids (GCs) are widely used in the treatment of immune-mediated diseases due to their anti-inflammatory and immunosuppressive effects. Prednisone is one most commonly GCs. However, it still unknown whether prednisone affects gut fungi rats. Herein we investigated changed composition interactions between mycobiome bacteriome/fecal metabolome Twelve male Sprague-Dawley rats were randomly assigned a control group which received daily by gavage for 6 weeks. ITS2 rRNA gene sequencing...
This study aimed to evaluate the clinical value of micro-proteinuria in combination with ultrasonography left renal vein (LRV) diagnosis orthostatic proteinuria (OP).The patients suspected OP received West test, upright lordotic position Robinson LRV, and detection morning urine after activity. The sensitivity (Se), specificity (Sp), positive negative predictive values (PPV NPV), likelihood ratios (PLR NLR) Youden's index (YI) for micro-proteinuria, LRV both them were analyzed.From January...
Abstract Primary nephrotic syndrome (PNS) is one of the most common primary glomerular diseases in children. Patients complicated with pancreatic lesions are rarely reported, and clinical manifestations children atypical. This study has observed incidence, types, prognosis acute pancreatitis (AP) syndrome, analyzed its related factors, early diagnosis, treatment. Seven PNS AP Shanghai Children's Hospital from January 2015 to December 2017 were reviewed. The data including age, height,...
This study is aimed at exploring the mechanism by which -254C>G single nucleotide polymorphism (SNP) on transient receptor potential cation channel 6 (TRPC6) gene promoter could increase its activation in steroid-resistant nephrotic syndrome children of China. Plasmids containing TRPC6 region (with -254C or G allele) were constructed and then transfected into human embryonic kidney (HEK) 293T cells podocytes. Luciferase assays used to test activity both cell lines with without tumor necrosis...
Rituximab is emerging as a new steroid sparing agent in children with difficult-to-treat nephrotic syndrome due to its ability of depleting CD20-positive B cells. Life-threatening adverse events such pneumocystis carinii pneumonia may occur even though it seems be well tolerated. Since rituximab wildly used immune-mediated diseases, important manage severe events. To explore the importance early diagnosis and treatment primary (PNS) after receiving therapy, we retrospectively analyzed...
Objective: To analyze the clinical characteristics of 6 children with TTC21B-related nephronophthisis to provide reference for early diagnosis. Methods: The general condition, manifestations, laboratory tests and other data from 4 families diagnosed by genetic testing in Shanghai Children's Hospital January 2015 December 2020 were analyzed retrospectively. Results: A total (3 males 3 females) developed proteinuria progressive renal dysfunction infancy. onset age was 18 (6, 25) months. at...
Objective: To explore the clinical features and expression of PLA(2)R in renal tissue children with idiopathic membranous nephropathy. Methods: Retrospective study was performed patients nephropathy diagnosed through biopsy follow-up time at least half a year Shanghai Children's Hospital from January 2010 to February 2017. We compared their clinicopathological pathological findings IMN. Indirect immunofluorescence assay used detect glomerular expression. analyzed differences between negative...
State space model (SSM) provides a general and flexible forecasting framework for time series. Conventional SSM with fixed-order Markovian assumption often falls short in handling the long-range temporal dependencies and/or highly non-linear correlation time-series data, which is crucial accurate forecasting. To this extend, we present External Memory Augmented Space Model (EMSSM) within sequential Monte Carlo (SMC) framework. Unlike common SSM, our features an external memory system, store...
Endemic hemolytic uremic syndrome (eHUS) is a worldwide problem and its pathophysiology not fully elucidated. We hypothesized that mannose‐binding lectin (MBL2), an initiating factor of complement pathway activation, plays crucial role in eHUS. generated novel human MBL2 expressing mouse (MBL2 KI) lacks murine MBLs ( +/+ Mbl1 ‐/‐ Mbl2 ). The eHUS model was designed with KI mice injected (day 0) shiga toxin‐2 (Stx‐2; 125 pg/g, ip) or without anti‐MBL2 antibody (3F8; 30 µg/g, ip). Blood cell...