N6-methyladenosine (m6A) is the most abundant internal modification on eukaryotic mRNA and gained increasing attention recently. More more evidence suggest that m6A methylation plays crucial role in tumor genesis development. However, its prostate cancer remains largely unknown. METTL3 expression status was analyzed by using TCGA database Western blotting. content RNA Methylation Quantification Kit. The of cells determined proliferation, survival, colony formation, invasion assays. level...

Oral squamous cell carcinoma (OSCC) is a common malignancy of the oral cavity. As survival rate OSCC patients low, it crucial to explore new markers and therapeutic targets for early diagnosis disease. A high level actinin alpha 1 (ACTN1) in could serve as an independent prognostic factor acute myeloid leukemia. However, role ACTN1 remains unclear. In present study, we aimed investigate OSCC.ACTN1 protein levels tissues were determined by immunohistochemical (IHC) staining. The correlation...

The incidence of bladder cancer in traditional green tea-consuming countries was dramatically lower than low countries. Epigallocatechin-3-gallate (EGCG), an active ingredient extracted from tea, showed effective inhibition formation and progression many tumors. However, whether autophagy involved this tumor-suppression mechanism EGCG on still unclear. In study, we demonstrated concentration EGCG-induced proliferation increased apoptosis cell lines (5,637 T24 cells) indicated by the...

The R2R3-MYB transcription factor (TF) family regulates metabolism of phenylpropanoids in various plant lineages. Species-expanded or specific MYB TFs may regulate species-specific metabolite biosynthesis including phenylpropanoid-derived bioactive products. Camellia sinensis produces an abundance specialized metabolites, which makes it excellent model for digging into the genetic regulation plant-specific biosynthesis. most abundant health-promoting metabolites tea are galloylated...

MicroRNA (miR)-202 has been reported to be involved in the regulation of human cancer progression including bladder cancer, non-small cell lung pancreatic and esophageal squamous carcinoma. However, function miR-202 prostate remains largely unknown. The present study demonstrated that was downregulated tissues lines. And overexpression significantly inhibited proliferation, migration invasion cells, but induced apoptosis. Moreover, suppressed tumor growth vivo. Regarding underlying...

This study aimed to construct a long noncoding RNA (lncRNA)-based prognostic signature improve the survival prediction for endometrial cancer (EC) patients and guide individualized treatments. mRNA miRNA sequencing clinical data of 526 with EC (randomized training or validation set, n = 263) were collected from The Cancer Genome Atlas database. Differentially expressed genes (DEGs), differentially lncRNAs (DELs), miRNAs (DEMs) identified between 263 samples 33 normal controls. Univariate...

Transient receptor potential melastatin subfamily member 7 (TRPM7) was essential in the growth and metastatic ability of prostate cancer cells. However, effects relevant molecular mechanisms TRPM7 on metastasis under hypoxic atmosphere remain unclear. This study investigated role androgen-independent cells hypoxia. First, data mining carried out to disclose relationship between gene level survival patients. Specific siRNAs were used knockdown target genes. Western blotting qPCR employed...

Leaf trichomes play vital roles in plant resistance and the quality of tea. Basic helix-loop-helix (bHLH) transcription factors (TFs) an important role regulating development growth. In this study, a total 134 CsbHLH proteins were identified Camellia sinensis var. (CSS) genome. They divided into 17 subgroups according to Arabidopsis thaliana classification. Phylogenetic tree analysis indicated that members IIIc-I IIIc-II might be associated with trichome formation. The expression patterns...

The plateaus of Tibet have a unique climate that poses great challenge for local agriculture. To promote sustainable development in Tibet, an elite tea variety has been introduced. However, the modifications flavors response to are unknown. In this study, volatile organic compounds (VOCs) and other taste substances planted its original location (OOT) (TOT) were systematically analyzed compared. components TOT OOT showed slight difference, principal component analysis revealed characteristic...

The CASP8 -652 6N insertion/deletion (I/D) polymorphism reduces expression of caspase 8. We conducted a meta-analysis to clarify the relationship between this and cancer risk. Eligible articles were retrieved from PubMed, EMBASE, CNKI, WANFANG databases through February 2017. A total 33 with 49 studies, including 33,494 cases 36,397 controls, analyzed. found that ins/del was associated decreased overall risk in five genetic models [DD vs. II: odds ratio (OR)=0.76, 95% confidence interval...

Prostate cancer (PCa) is the most common malignancy and leading cause of death in men. Recent studies suggest molecular signature was more effective than clinical indicators for prognostic prediction, but all known focused on a single RNA type. The present study to develop new by integrating long noncoding RNAs (lncRNAs) messenger (mRNAs) evaluate its performance.The expression data PCa patients were downloaded from Cancer Genome Atlas (TCGA) or Gene Expression Omnibus database (GSE17951,...

The mechanism of interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear to date, but reports showed that bladder inflammation and increasing number activating mast cells in tissues were common patients with IC/BPS. Houttuynia cordata is widely used Chinese traditional medicine, its function anti-inflammation has been proved. purpose this study was investigate the efficacy possible mechanisms (HC) extract treatment (IC/BPS). In current study, a total 30 adult female rats...

In the increasing demand for virus vaccines, large-scale production of safe, efficient, and economical viral antigens has become a significant challenge. High-cell-density manufacturing processes are most commonly used to produce vaccine protein drugs. However, cellular stress response in cell culture may directly affect host growth metabolism, reducing antigen costs. This study provided novel strategy antioxidant auxiliary system (AAS) supply molecular hydrogen (H2) into media via proton...

Background The specific mechanism of cardiovascular and cerebrovascular vasculopathy in the context end-stage renal disease has not been elucidated. In present study, we investigated clinical impact myeloid-derived suppressor cells (MDSCs) on hemodialysis patients their action. Methods MDSCs were tested among 104 undergoing association with overall survival (OS) events was determined. Results Hemodialysis presented a significantly higher level monocytic (M-MDSCs) compared to healthy...

Rice chromosome single segment substitution line (SSSL) W23-19-06-06-11 with the genotype Rf3Rf3/Rf4Rf4, a strong restorer for wild-abortive (WA) cytoplasmic male sterility (CMS), was recently identified from SSSL library. To investigate genetic mode of Rf genes and relationship among WA, yegong (Y), dwarf-wildabortive (DA) CMS systems, plants derived three BC3F2 populations involving lines, that carried Rf3Rf3/rf4rf4, rf3rf3/Rf4Rf4 genotypes WA-, Y-, DA-CMS cytoplasm, were selected their...

BACKGROUND Interstitial cystitis (IC) is a recurrent and chronic inflammatory disease that compromises patients' quality of life. Effective treatments for IC are limited. This study aimed to evaluate the therapeutic potency human umbilical cord-derived mesenchymal stem cells (UC-MSCs) in an IC-induced rat model investigate potential molecular mechanism mast cell (rat basophilic leukemia cells, RBL-2H3) treating coculture system. MATERIAL AND METHODS The was induced by cyclophosphamide (CYP)....

Primitive neuroectodermal tumor (PNET) rarely occurs as a primary renal neoplasm. Renal (r)PNET is rare but aggressive neoplasm with poor prognosis; the majority of patients are diagnosed advanced stage at presentation and face worse prognosis than localized disease. The present study describes diagnosis management eight cases rPNET an stage, who were treated two institutions [Lingnan Hospital (branch Third Affiliated Hospital) Cancer Center Sun Yat-sen University, Guangzhou], from December...

DNA methylation has been implicated as an important mechanism for the development of uterine corpus endometrial carcinoma (UCEC), indicating that methylation-driven genes may be potential biomarkers survival prediction. In this study, we aimed to identify a new prognostic signature UCEC based on differentially expressed (DEGs) and long noncoding RNAs (lncRNAs) (DELs). Sample-matched RNA-sequencing methylation-array data were downloaded from The Cancer Genome Atlas database, by analysis which...

Radical prostatectomy is the main treatment for prostate cancer (PCa), a common type among men. Recurrence frequently occurs in proportion of patients. Therefore, there great need to early screen those patients specifically schedule adjuvant therapy improve recurrence-free survival (RFS) rate. This study aims develop biomarker predict RFS with PCa based on data methylation, an important heritable contributor carcinogenesis. Methylation expression were downloaded from The Cancer Genome Atlas...