A5059102477- Environmental Toxicology and Ecotoxicology
- Pesticide Residue Analysis and Safety
- Pesticide and Herbicide Environmental Studies
- Analytical chemistry methods development
- Reproductive biology and impacts on aquatic species
- Marine Biology and Environmental Chemistry
- Pharmaceutical and Antibiotic Environmental Impacts
- Advanced biosensing and bioanalysis techniques
- Insect and Pesticide Research
- Zebrafish Biomedical Research Applications
- Transcranial Magnetic Stimulation Studies
- Neurobiology and Insect Physiology Research
- Sleep and Wakefulness Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biosensors and Analytical Detection
- Pharmacological Effects and Assays
- Aquaculture Nutrition and Growth
- Antibiotics Pharmacokinetics and Efficacy
- Functional Brain Connectivity Studies
- Pesticide Exposure and Toxicity
- Chemical Analysis and Environmental Impact
- Fish Ecology and Management Studies
- Sleep and related disorders
- EEG and Brain-Computer Interfaces
- Dementia and Cognitive Impairment Research
Zhejiang University
2016-2025
Ministry of Agriculture and Rural Affairs
2025
Institute of Psychology, Chinese Academy of Sciences
2018-2024
University of Chinese Academy of Sciences
2018-2024
Ningbo University
2024
Anhui University of Technology
2023
Chinese Academy of Sciences
2022
Gansu Agricultural University
2019-2020
Southwest University
2018
Michigan State University
2016
The widely used triazole fungicides have the potential to disrupt endocrine system, but little is known of such effects or underlying mechanisms hexaconazole (HEX) and tebuconazole (TEB) in fish. In present study, zebrafish (Danio rerio) embryos were exposed various concentrations HEX (0.625, 1.25 2.5 mg/L) TEB (1, 2 4 from fertilization 120 h post-fertilization (hpf). whole body content thyroid hormone transcription genes hypothalamic-pituitary-thyroid (HPT) axis analyzed. results showed...
The present study developed an improved analytical method for simultaneous quantification of seven neonicotinoids in food by ultraperformance liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry (UPLC−MS/MS) under the multiple reaction monitoring (MRM) mode. optimization extraction, cleanup, UPLC separation and MS/MS parameters analytes were especially focused on. low limits (LOQs) ranged from 0.1 to 6 μg kg−1. Meanwhile, reasonable...
The zebrafish (Danio rerio) has been used as a model for the study of endocrine disrupting chemicals. This set out to determine profiles whole-body thyroxine (T4) and 3,5,3'-triiodothyronine (T3) levels during development from embryo adult. Enzyme-linked immunoassay was analyze T4 T3 contents. results showed that remained stable pre-hatching period (0–3 d) increased significantly early after hatching. level peaked at 0.28 ± 0.01 ng g-1 body weight 10 days post-fertilization (dpf), 0.58 0.09...
Deltamethrin (DM) is a widely used insecticide that has demonstrated developmental toxicity in the early life stages of fish. To better characterize underlying mechanisms, embryos from Tg(cmlc2:RFP), Tg(apo14:GFP), and Tg(mpx:GFP) transgenic strains zebrafish were exposed to nominal DM concentrations 0.1, 1, 10, 25, 50 μg/L until 120 h post-fertilization (hpf). Heart size increased 56.7%, liver was reduced by 17.1% 22.7 24.2 DM, respectively. RNA sequencing bioinformatic analyses predicted...
Abstract The herbicide acetochlor is widely used and detected in the environment biota, has been suspected to disrupt thyroid endocrine system, but underlying mechanisms have not yet clarified. In present study, zebrafish larvae (7 days post‐fertilization) were exposed a series concentration of (0, 1, 3, 10, 30, 100 300 µg l −1 ) within 14‐day window until 21 post‐fertilization. Thyroid hormones mRNA expression profiles genes involved hypothalamic–pituitary–thyroid (HPT) axis analyzed....
A direct competitive enzyme-linked immunosorbent assay (ELISA) for triazophos was developed, which based on the THHe monoclonal antibody (McAb) and a heterologous enzyme tracer (THBu-HRP). The influence of several physicochemical factors (temperature, time, pH, salt, detergent, solvent) immunoassay studied. For standard curve, an I50 0.21μg/L limit detection (I20) 0.02μg/L obtained in high salt concentration buffer (0.05 M PBS, pH 6.0) with 0.05% BSA, means almost 3-fold improvement...
A broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides was raised using heterologous indirect enzyme-linked immunosorbent assay (ELISA) to screen hybridomas. On the basis of this Mab, five coating antigens were used develop homologous and competitive ELISAs. With most suitable competitor, a sensitive ELISA developed. The IC50 values estimated be 20.32 ng/mL parathion, 21.44 methyl-parathion, 42.15 fenitrothion, 58.85 isocarbophos. Spike recoveries between 70.52...