A5101976936- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Glioma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Adhesion Molecules Research
- Virology and Viral Diseases
- Congenital gastrointestinal and neural anomalies
- Viral-associated cancers and disorders
- Ophthalmology and Eye Disorders
- Toxin Mechanisms and Immunotoxins
- Cancer-related molecular mechanisms research
- Xenotransplantation and immune response
- Gastrointestinal motility and disorders
- Growth Hormone and Insulin-like Growth Factors
- Anesthesia and Neurotoxicity Research
- Poxvirus research and outbreaks
- Cardiac Ischemia and Reperfusion
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2022
Fudan University
2021-2022
Eye & ENT Hospital of Fudan University
2021-2022
Kunming Medical University
2020-2021
Nanjing Agricultural University
2017-2020
Novartis (China)
2017
Zhejiang University
2001-2016
University of South China
2014-2015
National Institutes of Health
2009-2013
Ningxia Medical University
2013
Glioblastoma multiforme (GBM) is the most common form of malignant glioma, characterized by genetic instability, intratumoral histopathological variability, and unpredictable clinical behavior. We investigated global gene expression in surgical samples brain tumors. Gene profiling revealed large differences between normal tumor tissues GBMs lower-grade oligodendroglial Extensive were found among GBMs, particularly genes involved angiogenesis, immune cell infiltration, extracellular matrix...
Glioblastoma multiforme (GBM) is the most common and lethal primary brain tumor in adults. We combined neuroimaging DNA microarray analysis to create a multidimensional map of gene-expression patterns GBM that provided clinically relevant insights into biology. Tumor contrast enhancement mass effect predicted activation specific hypoxia proliferation programs, respectively. Overexpression EGFR, receptor tyrosine kinase potential therapeutic target, was also directly inferred by validated an...
The infected cell protein (ICP)0 enables gene expression and the replication of herpes simplex virus (HSV)-1 in cells at low multiplicities enhances genes introduced into by transfection or infection. We report that a short sequence ICP0 is similar to amino terminus CoREST, corepressor exists complexes with repressor REST histone deacetylases (HDACs) 1 2 repress cellular expression. In wild-type-virus-infected cells, HDAC1 dissociates from CoREST/REST complex, CoREST are phosphorylated...
ABSTRACT Cellular processes requiring access to the DNA genome are regulated by an overlay of epigenetic modifications, including histone modification and chromatin remodeling. Similar cellular host, many nuclear viruses that depend upon host cell’s transcriptional machinery also subject regulatory impact assembly modification. Infection cells with alphaherpesviruses (herpes simplex virus [HSV] varicella-zoster [VZV]) results in deposition nucleosomes bearing repressive H3K9 methylation on...
The zebrafish (Danio rerio) has been used as a model for the study of endocrine disrupting chemicals. This set out to determine profiles whole-body thyroxine (T4) and 3,5,3'-triiodothyronine (T3) levels during development from embryo adult. Enzyme-linked immunoassay was analyze T4 T3 contents. results showed that remained stable pre-hatching period (0–3 d) increased significantly early after hatching. level peaked at 0.28 ± 0.01 ng g-1 body weight 10 days post-fertilization (dpf), 0.58 0.09...
Chromatin and the chromatin modulation machinery not only provide a regulatory matrix for enabling cellular functions such as DNA replication transcription but also regulate infectious cycles of many viruses. Elucidation components mechanisms involved in this regulation is providing targets development new antiviral therapies. Initiation infection by herpes simplex virus (HSV) requires activity several modification enzymes including histone demethylases LSD1 family JMJD2 proteins that...
An earlier report showed that the expression of viral genes by a herpes simplex virus 1 mutant [HSV-1(vCPc0)] in which wild-type, spliced gene encoding infected-cell protein no. 0 (ICP0) was replaced cDNA copy is dependent on both cell type and multiplicity infection. At low multiplicities infection, rabbit skin cells delayed many hours, although ultimately yield comparable to wild-type virus. This defect rescued replacement with gene. To test hypothesis delay reflected dysfunction ICP0...
In this study, we investigated the localization, morphological features and cellular interactions of telocytes in rat testicular interstitium. Transmission electron microscopy (TEM) immunohistochemical immunofluorescence analyses interstitium showed a distinct layer surround seminiferous tubules along with inner peritubular myoid cells. The majority were made up small cell body moniliform prolongations that contained mitochondria secretory vesicles. Some other observed possessing large...
Homocysteine (Hcy) has been recognized as a prevalent risk factor for cardiovascular events. Cholesterol-loaded foam cells are central component of atherosclerotic lesions. ATP-binding cassette transporter A1 (ABCA1), which mediates the efflux cellular cholesterol and phospholipids, is rate-limiting step in lipid metabolism. Acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT1) promotes accumulation ester macrophages, thereby resulting cell formation, hallmark early stage atherosclerosis. In...
Human Cytomegalovirus (hCMV) infects a broad range of the population and establishes life-long latency in infected individuals. Periodically latently virus can reactivate becomes significant cause morbidity mortality immunocompromised In latent infection, viral genome is suppressed heterochromatic state gene transcription silenced. Upon reactivation, repressive chromatin remodeled to an active form, allowing lytic transcription, initiated by expression Immediate Early (IE) genes. During this...
In order to trace the origin and evolution of avian infectious bronchitis virus (IBV) isolates in China Southeast Asia, genomic sequencing was used for molecular characterization 24 IBV two reference strains comparison with published sequences. The 5' region S1 genes, containing hypervariable regions I II, 3' nucleocapsid cytotoxic T lymphocyte epitopes, were construct phylogenetic trees analysis. results showed that could be divided into three distinct groups, is, American, Asian, European....
Infected cell protein 0 (ICP0) is a 775-residue multifunctional herpes simplex virus associated with numerous functions related to transactivation of gene expression and repression host defenses infection. We report that an uncharted domain ICP0 located between residues 245 510 contains multiple SH3 binding motifs similar those required for CIN85, the M r 85,000 interacts Cbl. CIN85 Cbl are involved in endocytosis negative regulation receptor tyrosine kinases. binds reciprocal manner...
Objective Thyroid-associated ophthalmopathy (TAO) is a disfiguring autoimmune disease, which destroys the structure of orbital tissues and even threatens vision. Metabolic reprograming critical in diseases; however, metabolic basis TAO remains to be clarified. Our study aimed reveal profile TAO. Methods Orbital adipose/connective from eleven patients twelve control subjects were collected during surgeries analyzed with liquid chromatograph-mass spectrometer. Orthogonal partial least-squares...
Oligodendroglioma (ODG) and oligoastrocytoma (OAC) are diffusely infiltrating primary brain tumors whose pathogenesis remains unclear. We previously identified a group of genes expression was inversely correlated with survival in cohort patients glioblastoma (GBM), some these also reportedly expressed ODG OAC. examined the patterns localization survival-associated OAC order to analyze their possible roles oncogenesis two tumor types.We used UniGene libraries derived from GBM specimens...
A stable recombinant fowl poxvirus (rFPV) expressing the C-terminal region (119 amino acids) of nucleocapsid (N) protein an infectious bronchitis virus (IBV) strain Ch3 was constructed by inserting coding sequence within thymidine kinase gene (FPV) homologous recombination. The N expressed under control vaccinia promoter P7.5 in chicken embryo fibroblast cell cultures as seen immunofluorescence assay and rFPV-inoculated specific-pathogen-free (SPF) chickens detecting antibodies with...
ABSTRACT An earlier report showed that infected cell protein no. 0 (ICP0) of herpes simplex virus 1 (HSV-1) interacts with the SH3 domains a recently discovered adaptor protein, CIN85. Here, we following. (i) ICP0 also other domain-containing proteins and, in particular, nonneuronal members Src kinase family. (ii) HSV-1 infection enhanced activating phosphorylation Tyr416 family, modestly activity Src, and posttranslationally modified at least one additional member family by manner dependent...