- Sperm and Testicular Function
- Turtle Biology and Conservation
- Autophagy in Disease and Therapy
- Animal Nutrition and Physiology
- Physiological and biochemical adaptations
- Zebrafish Biomedical Research Applications
- Lipid metabolism and biosynthesis
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Erythrocyte Function and Pathophysiology
- Gastrointestinal motility and disorders
- Toxoplasma gondii Research Studies
- Caveolin-1 and cellular processes
- Neuroendocrine regulation and behavior
- Rabbits: Nutrition, Reproduction, Health
- Ion Channels and Receptors
- Parasite Biology and Host Interactions
- Healthcare and Venom Research
- Reproductive biology and impacts on aquatic species
- Congenital gastrointestinal and neural anomalies
- Nuclear Structure and Function
- Metabolism and Genetic Disorders
- Moringa oleifera research and applications
- Reproductive Physiology in Livestock
- Selenium in Biological Systems
Nanjing Agricultural University
2018-2022
This study aimed to assess the effects of selenium and nanoparticles with chitosan on broiler chickens during heat stress. In this study, total 336 chicks were raised. These birds split into seven groups, each six sets eight birds, depending treatments they received. There two control groups: one regular diet (negative control) another plus stress (positive known as A B Groups respectively. The remaining groups follows: Group-C (Basal diet+0.3mg/kg selenium), Group-D + 0.3mg/kg nano stress),...
In this study, we investigated the localization, morphological features and cellular interactions of telocytes in rat testicular interstitium. Transmission electron microscopy (TEM) immunohistochemical immunofluorescence analyses interstitium showed a distinct layer surround seminiferous tubules along with inner peritubular myoid cells. The majority were made up small cell body moniliform prolongations that contained mitochondria secretory vesicles. Some other observed possessing large...
Steroidogenesis is an indispensable process that indirectly associated with spermatogenesis in the Leydig cell (LC) to utilize lipid droplets (LDs) are critical maintaining normal testosterone synthesis. The regulation of LD mobilization, known as lipophagy, LC still largely unknown.In present study, Chinese soft-shelled turtle was investigated identify steroidogenic activity and lipophagy during annual reproductive cycle by light microscopy, immunohistochemistry (IHC), immunofluorescence...
The existence of telocytes (TCs) has not yet been established in the pancreases aquatic reptiles. Here, we report TCs exocrine pancreas Pelodiscus sinensis using transmission electron microscope (TEM), immunohistochemistry (IHC), and immunofluorescence (IF) techniques. surrounded acini ducts connective tissue between lobules gland cells. cells were located preferably close to blood vessels, interlobular ducts, nerve fibers. Ultrastructurally, exhibited small large bodies with thick thin...
Telocytes (TCs) have been identified as a distinct type of interstitial cells, but not yet reported in the gastrointestinal tract (GIT) ruminants. In this study, we used transmission electron microscopy (TEM) and double-labelling immunofluorescence (IF) (antibodies: CD34, vimentin PGP9.5) to seek TCs investigate their potential functions muscle layers goat rumen. were distributed widely myenteric plexus (TC-MYs) between circular longitudinal layers, within (TC-CMs) well (TC-LMs)....
Spermatogenesis is a complex cellular process that includes many subcellular events are essential for the production of healthy spermatozoa. Autophagy physiological plays significant role in spermatogenesis; however, autophagy during avian spermatogenesis has not yet been reported. In current study, we characterized vivo throughout domestic fowl spermatogenesis. Autophagy-specific markers, including microtubule-associated protein light chain 3 (LC3), sequestosome 1 (p62), and...
Mitochondria are vital cellular organelles that have the ability to change their shape under different conditions, such as in response stress, disease, changes metabolic rate, energy requirements and apoptosis. In present study, we observed remodelling of mitochondria during spermiogenesis its relationship with mitochondria-associated granules (MAG). At beginning spermiogenesis, characterised by round shape. As progresses, round-shaped into elongated then swollen mitochondria, subsequently...
Melanomacrophagic centers (MMCs) were studied in the liver of zebrafish using transmission electron microscope (TEM). The MMCs located space Disse (SD), and their pseudopodia protruded into lumen sinusoids. degree extension body structure SD was determined by size phagocytosed content. An irregular or amoeboid nucleus present. Vacuoles occasionally present, both, endothelium MMCs. cytoplasm showed several engulfed structures. most common presence mitochondria small to giant distorted shape...
The epididymis plays a significant role as quality control organ for long-term sperm storage, maturation, and fertilizing ability perform filtration function to eliminate abnormal or residual spermatozoa by phagocytosis.However, the of autophagy in spermiophagy during storage turtle still needs be studied.In this study, we reported vivo via cellular evidence lysosome engulfment within principal cells epididymis.Using immunofluorescence, Lysosome associated membrane protein-1 (LAMP1)...
In this study, we investigated the neural changes and their relationships with interstitial cells (ICs) in rumen of pre-weaning goats by transmission electron microscopy, western blot immunofluorescence (antibody: general neuronal marker-Protein Gene Product (PGP9.5)/IC marker-vimentin).The results showed that PGP9.5-positive reaction was widely distributed soma (NS) nerve fibre (NF).The NSs were observed ganglia myenteric plexus (MP) but not submucosal plexus.The mean optical density (MOD)...
Although some studies have been conducted over the past few decades, existence of mitochondria-rich cells (MRCs) in reptiles is still obscure. This first study to uncover presence MRCs small intestine Chinese soft-shelled turtles. In this study, we investigated ultrastructural characteristics and secretion different ion transport proteins Pelodiscus sinensis. Transmission electron microscopy revealed that features are clearly from those other cells. The cytoplasmic density was higher than...