A5057137847- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Ultrasound Imaging and Elastography
- Ultrasonics and Acoustic Wave Propagation
- Glioma Diagnosis and Treatment
- Microfluidic and Bio-sensing Technologies
- Nanoplatforms for cancer theranostics
- Coronary Interventions and Diagnostics
- Microbial Inactivation Methods
- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Atherosclerosis and Cardiovascular Diseases
- Bacterial biofilms and quorum sensing
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nanoparticle-Based Drug Delivery
- 3D Printing in Biomedical Research
- Ultrasound and Cavitation Phenomena
- Meningioma and schwannoma management
- Venous Thromboembolism Diagnosis and Management
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Optical measurement and interference techniques
- Neonatal and fetal brain pathology
- Thermography and Photoacoustic Techniques
- Oral microbiology and periodontitis research
University of Maryland, Baltimore County
2025
University of Maryland, Baltimore
2016-2024
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2018-2024
University of Mary
2016-2024
University of Maryland Medical Center
2024
Veria General Hospital
2024
University of Hawaiʻi at Mānoa
2009-2014
University of Hawaii System
2011-2014
University of Hawaii Cancer Center
2014
Bioengineering Center
2012
Significance The blood–brain barrier (BBB) is a critical obstacle to delivering most therapeutics into the brain. In this study, we report combined MRI, histological, and real-time acoustic emissions monitoring findings from Phase 0 clinical trial examining microbubble-enhanced transcranial focused ultrasound for controlled BBB opening in patients with infiltrating gliomas. data revealed capability of technology provide safe, controlled, tightly monitored through intact skull. Our...
Focused ultrasound, when combined with circulating microbubbles (MB-FUS), leads to temporary neurovascular permeability and blood brain barrier opening (BBBO). Numerous trials using different devices, treatment parameters, monitoring technologies have created a need for approaches enabling comparative evaluation standardization of MB-FUS treatments. Analyzing 1028 unique sonications performed in patients gliomas, we investigated patient-specific, operator-adjusted, device-related variables...
Abstract Homeostatic regulation of the maternal milieu during pregnancy is critical for and fetal health. The placenta facilitates communication between compartments, in part, through production extracellular vesicles (EVs). EVs enable tissue synchrony via cell–cell long-distance are at their highest circulating concentration pregnancy. While much work has been done investigating how physiological challenges affect fetus, role placental health not well examined. We previously identified...
In this paper, we describe a new, high-frequency, time-resolved scanning acoustic microscope developed for studying dynamical processes in biological cells. The new operates mode. center frequency is 0.86 GHz, and the pulse duration 5 ns. With such short pulse, layers thicker than 3 mum can be resolved. For cell mum, front echo from substrate distinguished signal. Positions of first second pulses are used to determine local impedance modeled as thin liquid layer that has spatial variations...
Focused ultrasound (FUS)-mediated blood–brain barrier disruption (BBBD) can enable even large therapeutics such as stem cells to enter the brain from bloodstream. However, efficiency is relatively low. Our previous study showed that human neural progenitor (hNPCs) loaded with superparamagnetic iron oxide nanoparticles (SPIONs) in culture were attracted by an external magnetic field. In vivo, enhanced retention was observed near a magnet mounted on skull rat model of traumatic injury, where...
Infiltrating gliomas are challenging to treat, as the blood-brain barrier significantly impedes success of therapeutic interventions. While some clinical trials for high-grade have shown promise, patient outcomes remain poor. Microbubble-enhanced focused ultrasound (MB-FUS) is a rapidly evolving technology with demonstrated safety and efficacy in opening across various disease models, including infiltrating gliomas. Initially recognized its role augmenting drug delivery, potential MB-FUS...
Immuno-compromised patients such as those undergoing cancer chemotherapy are susceptible to bacterial infections leading biofilm matrix formation. This surrounding acts a diffusion barrier that binds up antibiotics and antibodies, promoting resistance treatment. Developing non-invasive imaging methods detect in the clinic needed. The use of ultrasound conjunction with targeted contrast agents (UCAs) may provide detection early stage formation facilitate optimal Ligand-targeted UCAs were...
Generating spatially controlled, non-destructive changes in the interstitial spaces of brain has a host potential clinical applications, including enhancing delivery therapeutics, modulating biological features within tissue microenvironment, altering fluid and pressure dynamics, increasing clearance toxins, such as plaques found Alzheimer's disease. Recently we demonstrated that ultrasound can non-destructively enlarge ex vivo. The goal current study was to determine whether these effects...
Triple-negative breast cancer (TNBC) patients with brain metastasis (BM) face dismal prognosis due to the limited therapeutic efficacy of currently available treatment options. We previously demonstrated that paclitaxel-loaded PLGA–PEG nanoparticles (NPs) directed Fn14 receptor, termed "DARTs", are more efficacious than Abraxane─an FDA-approved paclitaxel nanoformulation─following intravenous delivery in a mouse model TNBC BM. However, precise basis for this difference was not investigated....
High-frequency focused ultrasound has emerged as a powerful modality for both biomedical imaging and elastography. It is gaining more attention due to its capability outperform many other modalities at submicron resolution. Besides imaging, high-frequency or acoustic biomicroscopy been used in wide range of applications assess the elastic mechanical properties tissue single cell level. The interest microscopy stems from awareness relationship between biomechanical underlying biochemical...
High-frequency, scanning acoustic microscopy (SAM) imaging of the dividing HeLa cells (cell line derived from human cervics carcinoma) has revealed that acoustical images become darker during cell division. The dark contrast was attributed to increase sound attenuation in cytoskeleton. In this report, RF-imaging adherent at different stages cycle been conducted using high-frequency, time-resolved microscope (SAM). B-Scan show distinct patterns inside cell. Velocity longitudinal wave,...
The clinical applications of transcranial focused ultrasound continue to expand and include ablation as well drug delivery in the brain, where treatments are typically guided by MRI. Although MRI-guided systems also preferred for many preclinical investigations, they expensive purchase operate, require presence a nearby imaging center. For basic mechanistic studies, however, MRI is not required. purpose this study was design, construct, characterize evaluate portable, custom, laser-guided...
We present a unique case of retroperitoneal tumefactive fibroinflammatory lesion related to IgG4-sclerosing disease; it is rare manifestation the IgG4-related disease, which usually causes diffuse fibrosis when located in retroperitoneum, rather than mass-like lesions. A 49-year-old man presented emergency department complaining abdominal pain and vomiting. Subsequent testing with ultrasound, CT, MRI revealed large mass unknown origin, heterogenous, concentric circles pattern best visualized...
In the absence of clinically available biomarkers for CNS malignancies, conventional method disease monitoring in glioma patients is radiologic. While most cancers shed cell-free molecules tumor-derived DNA into circulation, brain tumors form exception to this rule, rarely shedding detectable levels tumor bloodstream. Microbubble-enhanced focused ultrasound (MB-FUS) a rapidly emerging clinical tool safely open blood-brain barrier (BBB) enhanced neurotherapeutic delivery and liquid biopsy....
Targeted ultrasound contrast agents (UCA) offer a novel, potential method for diagnostic imaging of biofilm infections. Currently, there is no established molecular in vitro infections with any type modality. For infective endocarditis, the case formation on damaged or diseased heart valves, an early might greatly reduce associated high mortality rate. Fluorescently labeled lectins were used targeted conjunction epifluorescence microscopy and time-resolved scanning acoustic visualization...
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Scanning acoustic microscopy (SAM), particularly time-resolved microscopy, is one of the few techniques for study mechanical properties only cell’s interior, cytosol and nucleus. Unfortunately, microscopes typically do not provide sufficient resolution to elasticity single cells. We demonstrate that high-frequency, microscope developed at Fraunhofer Institute Biomedical Technology (IBMT), Germany, capable imaging characterizing elastic micron size structures in cytoskeleton with a...