A5055613227- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Sarcoma Diagnosis and Treatment
- Image Processing Techniques and Applications
- Neurobiology and Insect Physiology Research
- Vascular Tumors and Angiosarcomas
- Ultrasound and Hyperthermia Applications
- Bone Tumor Diagnosis and Treatments
- Head and Neck Surgical Oncology
- IgG4-Related and Inflammatory Diseases
- Neuroendocrine Tumor Research Advances
- Photoacoustic and Ultrasonic Imaging
- Glioma Diagnosis and Treatment
- Medical Image Segmentation Techniques
- Multiple Myeloma Research and Treatments
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Infective Endocarditis Diagnosis and Management
- Neurofibromatosis and Schwannoma Cases
- Cholangiocarcinoma and Gallbladder Cancer Studies
- PARP inhibition in cancer therapy
- Urinary and Genital Oncology Studies
- Extracellular vesicles in disease
- Oral and Maxillofacial Pathology
- Hepatocellular Carcinoma Treatment and Prognosis
Washington University in St. Louis
2018-2024
University of Maryland, Baltimore
2023-2024
University of Maryland Medical Center
2024
University of Washington
2021
Bipar
2020-2021
University of Miami
2014-2019
Barnes-Jewish Hospital
2018
Strain-promoted click chemistry of nucleosides and nucleotides with an azido group directly attached to the purine pyrimidine rings various cyclooctynes in aqueous solution at ambient temperature resulted efficient formation (3 min 3 h) fluorescent, light-up, triazole products. The 2- 8-azidoadenine reacted fused cyclopropyl cyclooctyne, dibenzylcyclooctyne, or monofluorocyclooctyne produce products functionalized hydroxyl, amino, N-hydroxysuccinimide, biotin moieties. 5-azidouridine...
Background: Retroperitoneal sarcomas (RPS) are rare and primarily managed with surgery, which improves local recurrence-free overall survival. Radiation can improve control or provide palliation for inoperable metastatic RPS by eliciting tumor cell death via irreparable DNA damage. In extraordinary circumstances radiation-induced promotes immune-mediated regression of non-irradiated lesions in a process termed the abscopal effect. Abscopal effects incompletely understood, involving balance...
Infiltrating gliomas are challenging to treat, as the blood-brain barrier significantly impedes success of therapeutic interventions. While some clinical trials for high-grade have shown promise, patient outcomes remain poor. Microbubble-enhanced focused ultrasound (MB-FUS) is a rapidly evolving technology with demonstrated safety and efficacy in opening across various disease models, including infiltrating gliomas. Initially recognized its role augmenting drug delivery, potential MB-FUS...
Abstract Myxopapillary ependymomas ( MPE ) are considered benign (World Health Organization WHO grade I) neoplasms with favorable prognosis. However, malignant behavior occurs in a small subset. To our knowledge, only five anaplastic s have been reported without consensus on diagnostic criteria. We retrieved 14 from the pathology archives of six institutions. Each tumor included at least two following features: ≥5 mitoses per 10 high power fields, Ki‐67 labeling index LI ≥10%, microvascular...
We present a conditional random field for three-dimensional neurite tracing, using population of open curve active contours. aim at increased robustness under spatially varying neurite-background contrast, and the same time reducing computational complexity compared to state-of-the-art. While most existing contour based methods perform tracing by evolving multiple snakes along centerline in sequential manner, our approach implements simultaneous evolution, as we show theoretically algorithm...
We study the 3D neurite tracing problem in different imaging modalities. consider that examined images do not provide sufficient contrast between and background, signal-to-noise ratio varies spatially. first split stack into box sub-volumes, inside each we evolve simultaneously a number of open-curve snakes. The curves deform based on three criteria: local image statistics, shape smoothness, term enforces pairwise attraction snakes, given their spatial proximity shapes. validate our method...
Ewing sarcoma is a neoplasm within the family of small round blue cell tumors and most frequently arises from skeletal bone. Primary involvement central nervous system in these lesions extremely rare, with an incidence 1%.A case presented 34-year-old man who left facial numbness, multiple intracranial lesions, lumbar intradural lesion, diffuse spinal leptomeningeal involvement. A laminectomy biopsy were performed, which revealed diagnosis extraskeletal sarcoma/primitive neuroectodermal...
Protein interactions underlie the complexity of neuronal function. Potential between specific proteins in brain are predicted from assays based on genetic interaction and/or biochemistry. Genetic reveals endogenous, but not necessarily direct, proteins. Biochemistry-based assays, other hand, demonstrate direct proteins, often outside their native environment or without a subcellular context. We aimed to achieve best both approaches by visualizing protein directly within live animal. Here, we...
We study the development of neuronal morphology with respect to temporal correspondences among branching features. pose problem as a three-dimensional registration observed morphologies over time, given already calculated neurite traces, where we exploit inherent tree-like structures. align branches from pairs instances in hierarchical fashion, bigger smaller groups (subtrees), and alignment costs are accumulated along defined recursion depths calculate optimal correspondences. This way,...
Introduction: Pituitary adenomas occur in approximately 10% of the population and can cause hormonal imbalance compression neural structures adjacent to pituitary gland, most notably optic nerves chiasm. Although respond favorably treatment, some are locally invasive, treatment resistant, or even widely metastatic. To date, however, prognostication remains a controversial topic. Recently, epigenetic analysis has been reliably used categorize characterize wide range central nervous system...
We introduce a novel segmentation method for time-lapse image stacks of neurites based on the co-segmentation principle. Our aggregates information from multiple to improve task, using neurite model and tree similarity term. The takes into account branching characteristics, such as local shape smoothness continuity, while term exploits branch dynamics across stacks. approach improves accuracy in ambiguous regions, handling successfully out-of-focus effects bifurcations. validated our...
Abstract Adamantinomas are rare low-grade malignant bone tumors that composed of epithelial cells set in an osteofibrous stroma. Osteofibrous dysplasia-like and classic forms well known; however, sarcomatous transformation (dedifferentiation) is very rarely observed. We present the case a 48-year-old woman who presented with 5-cm proximal femoral lytic lesion at risk pathologic fracture. Frozen-section evaluation demonstrated intimate admixture atypical squamous nests spindle cells,...
Our goal is to analyze neuronal morphology during development using artificially created templates. Such templates serve as input from the domain expertise: a standardized representation of neuron, independent imaging modalities and resolution, what neurobiologist can provide knowledge qualitative observations in microscope. A template divided into context-specific (topology, shape, and/or function) compartments, our task segment neuron volumes their surroundings partition them accordingly....
Glioblastoma is the most common primary brain tumor with a poor prognosis despite advances in various treatment modalities, such as radiation therapy (RT). This study compared growth inhibition effects of focused ultrasound-targeted microbubble destruction (UTMD) RT using an orthotopic mouse glioma model. Mice were implanted GL261 glioblastoma cells and divided into three groups: control group (no treatment); (2 Gy/day, 5 days/week, 3 consecutive weeks); UTMD (FUS sonication presence...