A5014287900- Zebrafish Biomedical Research Applications
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Retinal Development and Disorders
- Muscle Physiology and Disorders
- Genetic Neurodegenerative Diseases
- Neurological Disorders and Treatments
- Advanced Fluorescence Microscopy Techniques
- Neurotransmitter Receptor Influence on Behavior
- Mitochondrial Function and Pathology
- Kruppel-like factors research
- Traumatic Brain Injury and Neurovascular Disturbances
- Angiogenesis and VEGF in Cancer
- Axon Guidance and Neuronal Signaling
- Congenital heart defects research
- Obsessive-Compulsive Spectrum Disorders
- Pluripotent Stem Cells Research
- Amyotrophic Lateral Sclerosis Research
- Aquaculture Nutrition and Growth
- Endoplasmic Reticulum Stress and Disease
- Cardiac Structural Anomalies and Repair
- Photoreceptor and optogenetics research
- Retinopathy of Prematurity Studies
Medical College of Wisconsin
2021-2025
University of California, Los Angeles
2009-2021
Neurobehavioral Systems
2017
Sichuan University
2013
University of Michigan–Ann Arbor
2005-2010
University of Minnesota
1999-2000
Abstract Dendritic and axonal morphology reflects the input output of neurons is a defining feature neuronal types 1,2 , yet our knowledge its diversity remains limited. Here, to systematically examine complete single-neuron morphologies on brain-wide scale, we established pipeline encompassing sparse labelling, whole-brain imaging, reconstruction, registration analysis. We fully reconstructed 1,741 from cortex, claustrum, thalamus, striatum other brain regions in mice. identified 11 major...
Abstract Characterizing the precise three-dimensional morphology and anatomical context of neurons is crucial for neuronal cell type classification circuitry mapping. Recent advances in tissue clearing techniques microscopy make it possible to obtain image stacks intact, interweaving neuron clusters brain tissues. As most current 3D reconstruction methods are only applicable single neurons, remains challenging reconstruct these digitally. To advance state art beyond challenges, we propose a...
ABSTRACT Ever since the seminal findings of Ramon y Cajal, dendritic and axonal morphology has been recognized as a defining feature neuronal types. Yet our knowledge concerning diversity morphologies, in particular distal projection patterns, is extremely limited. To systematically obtain single neuron full on brain-wide scale, we established platform with five major components: sparse labeling, whole-brain imaging, reconstruction, registration, classification. We achieved sparse, robust...
Axon regeneration within the mammalian central nervous system is extremely limited. In optic neuropathy conditions like glaucoma, inability of retinal ganglion cell (RGC) axons to regenerate a major impediment functional recovery. contrast, adult teleost fish such as zebrafish can fully RGC enabling visual recovery from nerve (ON) injury making it an ideal model probe mechanisms successful axon regeneration. Laser Capture Microdissection followed by RNA-sequencing (LCM-seq) was used identify...
Endothelial cells are developmentally derived from angioblasts specified in the mesodermal germ cell layer. The transcription factor etsrp/etv2 is at top of known genetic hierarchy for angioblast development. transcriptional events that induce etsrp expression and specification not well understood.We generated etsrp:gfp transgenic zebrafish used them to identify regulatory regions factors critical mesoderm.To investigate mechanisms initiate during embryogenesis, we have performed promoter...
Etsrp/Etv2 (Etv2) is an evolutionarily conserved master regulator of vascular development in vertebrates. Etv2 deficiency prevents the proper specification endothelial cell lineage, while its overexpression causes expansion lineage early embryo or embryonic stem cells. We hypothesized that alone capable transdifferentiating later somatic cells into Using heat shock inducible transgenic zebrafish, we demonstrate expression sufficient to transdifferentiate fast skeletal muscle functional blood...
Although the genetic cause for Huntington's disease (HD) has been known over 20 years, mechanisms that neurotoxicity and behavioral symptoms of this are not well understood. One hypothesis is N-terminal fragments HTT protein causative agents in HD peptide sequences adjacent to poly-glutamine (Q) repeats modify its toxicity. Here we test function 17 amino acids (N17) context exon 1 fragment a novel, inducible zebrafish model HD. Deletion N17 coupled with 97Q expansion (mHTT-ΔN17-exon1)...
The transcription factor Etsrp is required for vasculogenesis and primitive myelopoiesis in zebrafish. When ectopically expressed, etsrp sufficient to induce the expression of many vascular myeloid genes mammalian homolog etsrp, ER71/Etv2, also essential hematopoietic development. To identify downstream gain-of-function experiments were performed zebrafish embryos followed by profile analysis microarray. Subsequent vivo studies resulted identification fourteen with blood and/or expression,...
The transcription factor etsrp/Er71/Etv2 is a master control gene for vasculogenesis in all species studied to date. It also required hematopoiesis zebrafish and mice. Several novel genes expressed vasculature have been identified through transcriptional profiling of embryos overexpressing etsrp by microarrays. Here we re-examined this profile Illumina RNA-sequencing technology, revealing substantially increased number candidate regulated etsrp. Expression studies 50 selected from dataset...
Endothelial cells line the luminal surface of blood vessels and form a barrier between other tissues body. Ets variant 2 (ETV2) is transiently expressed in both zebrafish mice necessary sufficient for vascular endothelial cell specification. Overexpression this gene early mouse embryos results ectopic appearance cells. Ectopic expression ETV2 later development only subset responding to signal.We have examined pattern differentiating human embryonic stem (ESCs) determine when peak occurs. We...
Runx1 is sufficient but not required for cardiomyocyte cell cycle.
Anxiety and amygdalar stimulation may induce or exacerbate compulsions triggered by cortical-limbic hyperactivity, as in human obsessive-compulsive disorder (OCD). We previously created transgenic mice that exhibit OCD-like biting, movement behavioral perseverance abnormalities. These behaviors are caused expression of a neuropotentiating cholera toxin (CT) transgene dopamine D1 receptor-expressing (D1+) neurons within the intercalated nucleus (ICN) cortical areas project to orbitofrontal...
Abstract Ever since the seminal findings of Ramon y Cajal, dendritic and axonal morphology has been recognized as a defining feature neuronal types. Yet our knowledge concerning diversity morphologies, in particular distal projection patterns, is extremely limited. To systematically obtain single neuron full on brain-wide scale, we established platform with five major components: sparse labeling, whole-brain imaging, reconstruction, registration, classification. We achieved sparse, robust...
Seizures can be induced by systemic dopamine D1 receptor agonists or cortical-limbic neurostimulation non-selectively. are also often associated with tics and compulsions, which likewise involve hyperactivity. To determine if selective potentiation of receptor-expressing (D1+) neurons increases seizure susceptibility, we administered pentylenetetrazole (PTZ) to mice that express a neuropotentiating transgene only in glutamatergic, corticallimbic subset D1+ (D1CT-7 line). These exhibited...
Neuromuscular synaptogenesis is initiated by the release of agrin from motor neurons and activation receptor tyrosine kinase, MuSK, in postsynaptic membrane. MuSK gene expression regulated nerve-derived muscle activity. Agrin stimulates synapse-specific activating GABP(alphabeta) transcription factors endplate-associated myonuclei. In contrast, mechanism which activity regulates not known. We report on a 60-bp enhancer that confers promoter regulation differentiation, changes intracellular...
Dendritic and axonal morphology is a defining feature of neuronal types their connectivity. Yet our knowledge concerning the diversity extremely limited. To systematically obtain single neuron full on brain-wide scale in mice, we established pipeline that encompasses five major components: sparse labeling, whole-brain imaging, reconstruction, registration, classification. We achieved sparse, robust consistent fluorescent labeling by combining transgenic or viral Cre delivery with novel...