Stephanie Mok
A5036363800- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Pharmacological Effects of Natural Compounds
- Pain Mechanisms and Treatments
- Advanced Neuroimaging Techniques and Applications
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Metastasis and carcinoma case studies
- Receptor Mechanisms and Signaling
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Neuroendocrine Tumor Research Advances
- Viral Infections and Immunology Research
- Wnt/β-catenin signaling in development and cancer
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
- Neuroscience and Neuropharmacology Research
- Lanthanide and Transition Metal Complexes
- Lung Cancer Research Studies
- Pluripotent Stem Cells Research
- RNA modifications and cancer
Allen Institute for Brain Science
2019-2022
Allen Institute
2019-2022
Western University
2015
Harvard University
2010-2012
National Institutes of Health
2011
Abstract Dendritic and axonal morphology reflects the input output of neurons is a defining feature neuronal types 1,2 , yet our knowledge its diversity remains limited. Here, to systematically examine complete single-neuron morphologies on brain-wide scale, we established pipeline encompassing sparse labelling, whole-brain imaging, reconstruction, registration analysis. We fully reconstructed 1,741 from cortex, claustrum, thalamus, striatum other brain regions in mice. identified 11 major...
Abstract The neocortex is disproportionately expanded in human compared with mouse 1,2 , both its total volume relative to subcortical structures and the proportion occupied by supragranular layers composed of neurons that selectively make connections within other telencephalic structures. Single-cell transcriptomic analyses show an increased diversity glutamatergic neuron types pronounced gradients as a function cortical depth 3 . Here, probe functional anatomical correlates this diversity,...
An essential step toward understanding brain function is to establish a structural framework with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and systems can be integrated interpreted
SUMMARY The isocortex and hippocampal formation are two major structures in the mammalian brain that play critical roles perception, cognition, emotion learning. Both contain multiple regions, for many of which cellular composition is still poorly understood. In this study, we used complementary single-cell RNA-sequencing approaches, SMART-Seq 10x, to profile ∼1.2 million cells covering all regions adult mouse formation, derived a cell type taxonomy comprising 379 transcriptomic types....
ABSTRACT Ever since the seminal findings of Ramon y Cajal, dendritic and axonal morphology has been recognized as a defining feature neuronal types. Yet our knowledge concerning diversity morphologies, in particular distal projection patterns, is extremely limited. To systematically obtain single neuron full on brain-wide scale, we established platform with five major components: sparse labeling, whole-brain imaging, reconstruction, registration, classification. We achieved sparse, robust...
The neocortex is disproportionately expanded in human compared to mouse, both its total volume relative subcortical structures and the proportion occupied by supragranular layers that selectively make connections within cortex other telencephalic structures. Single-cell transcriptomic analyses of mouse show an increased diversity glutamatergic neuron types pronounced gradients as a function cortical depth. To probe functional anatomical correlates this diversity, we describe robust Patch-seq...
The isocortex and hippocampal formation are two major structures in the mammalian brain that play critical roles perception, cognition, emotion learning. Using single-cell RNA-sequencing approaches, we profiled ~1.2 million cells covering all regions adult mouse formation. cell types organized hierarchically exhibit varying degrees of discrete or continuous variations. Such molecular relationships correlate strongly with spatial distribution patterns types, which can be region-specific,...
Abstract An essential step toward understanding brain function is to establish a cellular-resolution structural framework upon which multi-scale and multi-modal information spanning molecules, cells, circuits systems can be integrated interpreted. Here, through collaborative effort from the Brain Initiative Cell Census Network (BICCN), we derive comprehensive cell type-based description of one structure - primary motor cortex upper limb area (MOp-ul) mouse. Applying state-of-the-art...
Abstract Ever since the seminal findings of Ramon y Cajal, dendritic and axonal morphology has been recognized as a defining feature neuronal types. Yet our knowledge concerning diversity morphologies, in particular distal projection patterns, is extremely limited. To systematically obtain single neuron full on brain-wide scale, we established platform with five major components: sparse labeling, whole-brain imaging, reconstruction, registration, classification. We achieved sparse, robust...
Dendritic and axonal morphology is a defining feature of neuronal types their connectivity. Yet our knowledge concerning the diversity extremely limited. To systematically obtain single neuron full on brain-wide scale in mice, we established pipeline that encompasses five major components: sparse labeling, whole-brain imaging, reconstruction, registration, classification. We achieved sparse, robust consistent fluorescent labeling by combining transgenic or viral Cre delivery with novel...
Abstract Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are malignant epithelial cancer arising from the diffuse system. Diagnosis is usually made late in disease course, with 60-80% of diagnosed cases presenting with, or developing metastatic disease. The lack biomarkers indicative aggressive behavior, particular metastasis, hinders prognosis and proper treatment GEP-NETs. A cellular process underlying behavior to mesenchymal transition (EMT). In this study, we aimed determine...
Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected sections for FPCR and/or RNA-seq analysis.
Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected sections for FPCR and/or RNA-seq analysis.
We have developed a multiplexed single molecule FISH protocol for use at the Institute. This was optimized on human tissue, but will work mouse tissue as well. It adapted from Lyubimova et. al., Nature Protocols, 2013.