A5077801015- Cell Image Analysis Techniques
- Neural dynamics and brain function
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Memory and Neural Mechanisms
- Cardiac Fibrosis and Remodeling
- Neuroscience and Neuropharmacology Research
- Cellular Mechanics and Interactions
- Near-Field Optical Microscopy
- Signaling Pathways in Disease
- Cell Adhesion Molecules Research
- Optical Coherence Tomography Applications
- Brain Tumor Detection and Classification
- Caveolin-1 and cellular processes
- Atherosclerosis and Cardiovascular Diseases
- Advanced Electron Microscopy Techniques and Applications
- Cell death mechanisms and regulation
- Bioinformatics and Genomic Networks
- Lipoproteins and Cardiovascular Health
- Neural Networks and Applications
University at Buffalo, State University of New York
2023-2025
Cold Spring Harbor Laboratory
2020-2023
An essential step toward understanding brain function is to establish a structural framework with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and systems can be integrated interpreted
Abstract Understanding how cortical circuits generate complex behavior requires investigating the cell types that comprise them. Functional differences across pyramidal neuron (PyN) have been observed within areas, but it is not known whether these local extend throughout cortex, nor additional emerge when larger-scale dynamics are considered. We used genetic and retrograde labeling to target tract, intratelencephalic corticostriatal projection neurons measured their cortex-wide activity....
Abstract An essential step toward understanding brain function is to establish a cellular-resolution structural framework upon which multi-scale and multi-modal information spanning molecules, cells, circuits systems can be integrated interpreted. Here, through collaborative effort from the Brain Initiative Cell Census Network (BICCN), we derive comprehensive cell type-based description of one structure - primary motor cortex upper limb area (MOp-ul) mouse. Applying state-of-the-art...
Vascular dysfunction is a common cause of cardiovascular diseases characterized by the narrowing and stiffening arteries, such as atherosclerosis, restenosis, hypertension. Arterial results from aberrant proliferation vascular smooth muscle cells (VSMCs) their increased synthesis deposition extracellular matrix (ECM) proteins. These, in turn, are modulated arterial stiffness, but mechanism for this not fully understood. We found that survivin an important regulator stiffness-mediated ECM...
Abstract Understanding how cortical circuits generate complex behavior requires investigating the cell types that comprise them. Functional differences across pyramidal neuron (PyN) have been observed within areas, but it is not known whether these local extend throughout cortex, nor additional emerge when larger-scale dynamics are considered. We used genetic and retrograde labeling to target tract (PT), intratelencephalic (IT) corticostriatal projection neurons measured their cortex-wide...
Abstract Brain research is an area of characterized by its cutting-edge nature, with brain mapping constituting a crucial aspect this field. As sequencing tools have played role in gene sequencing, largely depends on automated, high-throughput and high-resolution imaging techniques. Over the years, demand for has scaled exponentially rapid development microscopic mapping. In paper, we introduce novel concept confocal Airy beam into oblique light-sheet tomography named CAB-OLST. We...
Here we describe a new method, named LS-SOFI, that combines l ight- s heet fluorescence microscopy and uper-resolution o ptical f luctuation i maging to achieve fast nanoscale-resolution imaging over large fields of view in native 3D tissues. We demonstrate the use LS-SOFI super-resolution analysis neuronal structures synaptic proteins, including cortical axons, dendritic spines, pre- postsynaptic cytoskeletal proteins AMPA receptors, thick mouse brain sections. also introduce an algorithm...
ABSTRACT Cardiovascular diseases (CVDs) and pathologies are often driven by changes in molecular signaling communication, as well cellular tissue components, particularly those involving the extracellular matrix (ECM), cytoskeleton, immune response. The fine-wire vascular injury model is commonly used to study neointimal hyperplasia vessel stiffening, but it not typically considered a for CVDs. In this paper, we hypothesize that induces gene expression, biological processes similar observed...
Cell-type composition across brain regions is a critical structural factor shaping both local and long-range circuits. Here, we employed single-cell resolution imaging of the mouse brain, combined with computational analyses, to map distribution 30 cell classes types defined by gene marker expression in Cre recombinase-based genetic models. This approach generated comprehensive atlas type-specific densities male female revealing (1) surprisingly broad sex differences cells tagged...
Light microscopy and super-resolution are complementary imaging modalities, yet their integration into a single versatile method has been largely lacking. Here we describe new method, named LS-SOFI, that combines light-sheet fluorescence optical fluctuation to achieve fast nanoscale-resolution over large fields of view in 3D tissues organs. We demonstrate LS-SOFI neuronal structures synaptic proteins thick mouse brain sections, including cortical axons, dendritic spines, pre- postsynaptic...