A5052530754- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Chromosomal and Genetic Variations
- Cancer Immunotherapy and Biomarkers
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- DNA Repair Mechanisms
- Plant Disease Resistance and Genetics
- Viral gastroenteritis research and epidemiology
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related gene regulation
- Liver physiology and pathology
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- Colorectal and Anal Carcinomas
- CAR-T cell therapy research
- Immune Cell Function and Interaction
Rutgers, The State University of New Jersey
2012-2024
Cold Spring Harbor Laboratory
2005-2014
Universidad de Navarra
1998-2007
Science Oxford
2005
Universidad de Salamanca
2005
Pasadena City College
2005
Bethesda University
2005
Seattle University
2005
United States Department of Justice
2005
Small interfering RNA (siRNA) guides dimethylation of histone H3 lysine-9 (H3K9me2) via the Argonaute and RNA-dependent polymerase complexes, as well base-pairing with either or DNA. We show that requires conserved aspartate-aspartate-histidine motif for heterochromatic silencing ribonuclease H-like cleavage (slicing) target messages complementary to siRNA. In fission yeast Schizosaccharomyces pombe, repeats are transcribed by II. H3K9me2 spreads into silent reporter genes when they embedded...
Abstract We have constructed a recombinant defective adenovirus that expresses functional murine IFN-γ-inducible protein-10 (IP-10) chemokine (AdCMVIP-10). Injection of AdCMVIP-10 into s.c. tumor nodules derived from the CT26 colorectal adenocarcinoma cell line displayed some antitumor activity but it was not curative in most cases. Previous studies shown injection similar with adenovirus-encoding IL-12 (AdCMVIL-12) induces regression nearly 70% cases association generation CTL activity....
ABSTRACT Posttranscriptional gene silencing allows sequence-specific control of expression. Specificity is guaranteed by small antisense RNAs such as microRNAs (miRNAs) or interfering (siRNAs). Functional miRNAs derive from longer double-stranded RNA (dsRNA) molecules that are cleaved to pre-miRNAs in the nucleus and transported exportin 5 (Exp 5) cytoplasm. Adenovirus-infected cells express virus-associated (VA) RNAs, which dsRNA similar structure pre-miRNAs. VA also Exp cytoplasm, where...
Artificially enforced expression of CD80 (B7-1) and CD86 (B7-2) on tumor cells renders them more immunogenic by triggering the CD28 receptor T cells. The enigma is that such B7s interact with much higher affinity CTLA-4 (CD152), an inhibitory expressed activated We show unmutated spontaneously at low levels mouse colon carcinoma cell lines other transplantable various tissue origins. Silencing interfering RNA led to loss tumorigenicity CT26 in immunocompetent mice, but not immunodeficient...
<ns4:p>In the fission yeast <ns4:italic>Schizosaccharomyces pombe </ns4:italic>the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and selectable marker. The CRISPR/Cas9 system has recently been implemented in yeast, which allows seamless genome editing without integration selection marker or leaving any other ‘scars’. published method involves manual design single guide RNA (sgRNA), digestion large...
Parasitic DNA targets a genomic home Long-terminal-repeat (LTR) retrotransposons are form of parasitic that can jump around within the host's genome. To avoid damaging resident genes, they have been selected to integrate away from protein-coding sequences. For instance, fission yeast LTR retrotransposon Tf1 inserts at nucleosome-free regions in gene promoters. Jacobs et al. show is directed these insertion sites by specific binding protein, Sap1, which forms replication–fork barriers....
Abstract Transposable elements are molecular parasites that persist in their host genome by generating new copies to outpace natural selection. exert a large influence on evolution, some cases providing adaptive changes. Here we measure the fitness effect of transposable element insertions fission yeast Schizosaccharomyces pombe type strain removing all its only native family, long terminal repeat retrotransposon Tf2. We show Tf2 provide positive contribution host. ablation results changes...
In the fission yeast Schizosaccharomyces pombe, a chromosomal imprinting event controls asymmetric pattern of mating-type switching. The orientation DNA replication at locus is instrumental in this process. However, factors leading to are not fully identified and mechanism poorly understood. Here, we show that fork pause mat1 (MPS1), essential for imprint formation, depends on lysine-specific demethylase Lsd1. We demonstrate either Lsd1 or Lsd2 amine oxidase activity required these...
<ns4:p>In the fission yeast <ns4:italic>Schizosaccharomyces pombe </ns4:italic>the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and selectable marker. The CRISPR/Cas9 system has recently been implemented in yeast, which allows seamless genome editing without integration selection marker or leaving any other ‘scars’. published method involves manual design single guide RNA (sgRNA), digestion large...
Histone methylation regulates transcription, chromatin structure, and the epigenetic state of cell. Recent studies identified JmjC domain as a catalytic module for histone demethylation. Schizosaccharomyces pombe contains seven proteins, but it was unclear whether any them functioned demethylases. In this report, we show that protein Jmj2, which is evolutionarily conserved from yeast to human, reversed trimethylated H3-Lys-4 di- mono-but not unmethylated products. Overexpression Jmj2...
Repressive histone H3 lysine 9 methylation (H3K9me) and its recognition by HP1 proteins are necessary for pericentromeric heterochromatin formation. In Schizosaccharomyces pombe, H3K9me deposition depends on the RNAi pathway. Cryptic loci regulator 4 (Clr4), only known H3K9 methyltransferase in this organism, is a subunit of Clr4 complex (CLRC), whose composition reminiscent CRL4 type cullin-RING ubiquitin ligase (CRL) including cullin Cul4, RING-box protein Pip1, DNA damage binding 1...
Gene delivery of IFN-α to the liver may represent an interesting strategy maximize its antiviral efficacy and reduce side effects. We used a recombinant adeno-associated virus (AAV) encoding woodchuck (AAV-IFN) treat animals with chronic hepatitis infection. The vector was given by intraportal or intramuscular route. Long-term transgene expression detected after administration AAV luciferase. In contrast, in majority that received AAV-IFN through portal vein, transient (30–40 days)...
Abstract Sumoylation is required to repair protein-linked DNA damage, but its presence can limit the use of alternative pathways. Through a suppressor... Protein modification by small ubiquitin-like modifier (SUMO) plays important roles in genome maintenance. In Saccharomyces cerevisiae, proper regulation sumoylation known be essential for viability certain mutants. Here, we find opposite result; lethal Yeast cells lacking factors TDP1 and WSS1 are synthetically due their redundant removing...