A5014135006- Protease and Inhibitor Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Blood Coagulation and Thrombosis Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Antimicrobial Resistance in Staphylococcus
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular Health and Disease Prevention
- Cardiovascular Function and Risk Factors
- Cardiovascular Disease and Adiposity
- Blood properties and coagulation
- Cerebrovascular and Carotid Artery Diseases
- Cell Adhesion Molecules Research
- Peroxisome Proliferator-Activated Receptors
- Acute Myocardial Infarction Research
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Wound Healing and Treatments
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Nitric Oxide and Endothelin Effects
- Cardiac electrophysiology and arrhythmias
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Antiplatelet Therapy and Cardiovascular Diseases
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Bacterial Identification and Susceptibility Testing
Sapporo Medical University Hospital
2013-2025
Yokohama City University
1987-2025
Asahikawa Medical University
2014-2024
John Wiley & Sons (Germany)
2024
Yokohama City University Hospital
1998-2024
Asahi General Hospital
2023-2024
National Institute of Technology Okinawa College
2020
National Cancer Center Hospital East
2019
Nagoya City University
2008-2017
Hokkaido Pharmaceutical University
2017
bcl-x is a member of the bcl-2 gene family, which may regulate programmed cell death. Mice were generated that lacked Bcl-x. The Bcl-x-deficient mice died around embryonic day 13. Extensive apoptotic death was evident in postmitotic immature neurons developing brain, spinal cord, and dorsal root ganglia. Hematopoietic cells liver also apoptotic. Analyses double-knockout chimeric showed maturation lymphocytes diminished. life-span lymphocytes, but not mature shortened. Thus, Bcl-x functions...
The function of Aurora-C kinase, a member the Aurora kinase family identified in mammals, is currently unknown. We present evidence that Aurora-C, like Aurora-B chromosomal passenger protein localizing first to centromeres and then midzone mitotic cells. transcript expressed at moderate level albeit about an order magnitude lower than diploid human fibroblasts. elevated several cancer cell types. mRNA expressions are maximally during G2/M phase but their expression profiles synchronized...
Background Obesity is known to predispose attenuated fibrinolysis attributable increased concentrations in plasma of type-1 plasminogen activator inhibitor (PAI-1), the primary physiological endogenous fibrinolysis. PAI-1 present neointimal vascular smooth muscle cells and lipid-laden macrophages. Methods Results The study was designed determine whether expression occurs adipose tissue as well, thereby potentially contributing cardiovascular risk associated with obesity. 3T3-L1 preadipocytes...
Cellulose synthesis in plants is believed to be carried out by the plasma membrane-associated rosette structure which can observed electron microscopy. Despite decade-long speculation, it had not been demonstrated whether site of catalytic activity cellulose synthesis. To determine relationship between this and synthesis, we successfully isolated detergent-insoluble rosettes from membrane bean epicotyls. However, purified did possess vitro. Conversely, detergent-soluble granular particles...
Plasminogen activator inhibitor type-1 (PAI-1) plays an integral role not only in the regulation of fibrinolytic activity but also pathogenesis atherosclerosis and hypertension. We investigated signaling pathways angiotensin II (Ang II) leading to PAI-1 gene expression. Ang increased mRNA protein levels a time- dose-dependent manner through type 1 receptor vascular smooth muscle cells. promoter measured by luciferase assay was significantly II. stability II-induced upregulation inhibited...
Coronary artery disease (CAD) is a major problem worldwide. Atherosclerosis and thrombosis underlying CAD involve multiple cell types. New useful diagnostic markers are required. MicroRNAs (miRNAs) class of noncoding RNAs that posttranscriptionally regulate the gene expressions involved in various cellular processes. Endothelial dysfunction implicated early processes athero-thrombosis. Thus, it was hypothesized level vascular endothelium-enriched miRNAs would be altered plasma samples...
Staphylococcal superantigen-like proteins (SSLs) are a family of exoproteins sharing structural similarity with superantigens, but no superantigenic activity. Corresponding host target or receptors against portion SSLs in the have been identified. In this study, we show that SSL3 specifically binds to Toll-like receptor 2 (TLR2) and inhibits stimulation macrophages by TLR2 ligands. An approximately 100-kDa protein was recovered using recombinant His-tagged SSL3-conjugated Sepharose from...
Obesity is associated with hyperinsulinemia and elevated concentrations of tumor necrosis factor-α (TNF-α) in adipose tissue. TNF-α has been implicated as an inducer the synthesis plasminogen activator inhibitor-1 (PAI-1), primary physiological inhibitor fibrinolysis, mediated by activators cultured adipocytes. To identify mechanism(s) through which induces PAI-1, 3T3-L1 preadipocytes were differentiated into adipocytes exposed to for 24 h. selectively increased PAI-1 without increasing...
To identify factors potentially influencing expression of type 1 plasminogen activator inhibitor (PAI-1), we characterized the human tissue-specific distribution PAI-1 mRNA and influence epidermal growth factor (EGF) on steady state levels secretion by Hep G2 cells. Two species (3.2 2.2 kilobases) were detected, ratio two varied among tissues (3 to 5:1) in contrast 1:1 detected Expression was inversely related tissue-type (2.3 kilobases). Nu-Serum, a media supplement, increased 5-fold within...
Colletotrichum lagenarium is the causal agent of anthracnose cucumber. This fungus produces a darkly melanized infection structure, appressoria, to penetrate host leaves. The C. CMK1 gene, homologue Saccharomyces cerevisiae FUS3/KSS1 mitogen-activated protein (MAP) kinase genes, was shown regulate conidial germination, appressorium formation, and invasive growth. In S. cerevisiae, Ste12p known be transcriptional factor downstream Fus3p/Kss1p MAP kinases. To evaluate pathway, we isolated...
We aimed to assess the frequency of acute kidney injury (AKI) in different areas under concentration-time curve (AUC) values vancomycin (VAN) using a two-point blood collection method, allowing for accurate AUC assessment critically ill patients. This multicenter retrospective observational study was conducted eight hospitals. retrospectively analyzed data patients who had received VAN an intensive care unit (ICU) between January 2020 and December 2022. The primary outcome incidence AKI....
146 Background: Despite the introduction of anti-EGFR therapies, resistance remains a significant challenge in metastatic colorectal cancer (mCRC). MET amplification is recognized mechanism. Cabozantinib, multi-kinase inhibitor targeting MET, has shown promise overcoming to therapy. This study aimed evaluate efficacy and safety cabozantinib alone (Cabo) or combination with panitumumab (Cabo+Pmab) patients MET-amplified mCRC. Patients Methods: The population included RAS BRAF wild-type mCRC...
Abstract Objectives Recently, the EUROIMMUN anti‐acetylcholine receptor antibody (AChR Ab) enzyme‐linked immunosorbent assay (ELISA) became commercially available in Japan, but its clinical performance has not been comprehensively researched. This is first diagnostic evaluation of AChR Ab ELISA carried out Japanese population, including stratification myasthenia gravis (MG) patients according to length treatment. Methods Concentrations sera from clinically confirmed MG ( n = 85) and non‐MG...
Abstract Background Population pharmacokinetic (PPK) models of vancomycin (VCM) commonly use creatinine clearance (CLcr) as a covariate for (CL). However, relying on CLcr in patients advanced age may lead to inaccuracies estimating VCM clearance. Therefore, this study aimed develop and validate new PPK model specifically aged 75 years older. Methods analysis was performed based the blood concentrations ( n = 159 patients). The predictive performance developed compared with that previous...
Mouse allograft inflammatory factor-1 (AIF-1) cDNA was cloned and the AIF-1-specific monoclonal antibodies were established to examine its tissue distribution. The mouse AIF-1 highly conserved among all reported from a variety of species, invertebrates mammals, in good accordance with putative expressed regions genomic sequences major histocompatibility complex (MHC) class III region. messages abundantly testis, moderately spleen lymph nodes slightly liver thymus normal BALB/c mice....
Summary Fungal plant pathogens have evolved diverse strategies to overcome the multilayered defence responses that confront them upon host invasion. Here we show pathogenicity of cucumber anthracnose fungus, Colletotrichum lagenarium , and rice blast Magnaporthe grisea requires a gene orthologous Saccharomyces cerevisiae SSD1 regulator cell wall assembly. Screening for C. insertional mutants deficient in led identification ClaSSD1 . Following targeted replacement, appressoria classd1...
Peroxisomes are ubiquitous organelles of eukaryotic cells that fulfill a variety biochemical functions, including β-oxidation fatty acids. Here, we report an ortholog the Saccharomyces cerevisiae peroxisome biogenesis gene PEX13 is required for pathogenicity Colletotrichum orbiculare. CoPEX13 was identified by screening random insertional mutants deficiency in acid utilization. Targeted knockout were unable to utilize acids as carbon source. Expression analysis using green fluorescent...