A5034601674- Immune Cell Function and Interaction
- Atherosclerosis and Cardiovascular Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Cardiac Fibrosis and Remodeling
- Cardiovascular Function and Risk Factors
- Adipose Tissue and Metabolism
- Genetic and phenotypic traits in livestock
- Cardiovascular Disease and Adiposity
- Heart Failure Treatment and Management
- Protease and Inhibitor Mechanisms
- Cardiac Imaging and Diagnostics
- T-cell and B-cell Immunology
- Genetic Associations and Epidemiology
- Advanced MRI Techniques and Applications
- Chemotherapy-induced cardiotoxicity and mitigation
- Muscle metabolism and nutrition
- Liver Disease Diagnosis and Treatment
- Signaling Pathways in Disease
- Cardiac Ischemia and Reperfusion
- Cardiovascular and exercise physiology
- Blood properties and coagulation
- Chronic Kidney Disease and Diabetes
- Diet and metabolism studies
- Chemokine receptors and signaling
Hokkaido University
2011-2024
Hokkaido University Hospital
2016
American Heart Association
2013
Diponegoro University
2012
Nippon Zoki (Japan)
2012
Chiba University
2012
Kitasato University
2012
RIKEN Center for Integrative Medical Sciences
2012
Jackson Laboratory
2003-2009
Nagoya City University
2009
Background— Macrophage and lymphocyte infiltration in adipose tissue may contribute to the pathogenesis of obesity-mediated metabolic disorders. Natural killer T (NKT) cells, which integrate proinflammatory cytokines, have been demonstrated atherosclerotic lesions visceral tissue. Objective— To determine whether NKT cells are involved glucose intolerance inflammation diet-induced obese mice. Methods Results— development intolerance, male β 2 -microglobulin knockout (KO) mice lacking C57BL/6J...
The progression of obesity is accompanied by a chronic inflammatory process that involves both innate and acquired immunity. Natural killer T (NKT) cells recognize lipid antigens are also distributed in adipose tissue. To examine the involvement NKT development obesity, C57BL/6 mice (wild type; WT), two NKT-cell-deficient strains, Jα18(-/-) lack type I subset CD1d(-/-) II subsets, were fed high fat diet (HFD). gained least body weight with perigonadal brown tissue as well liver, compared to...
Chronic inflammation in the myocardium is involved development of left ventricular (LV) remodeling and failure after myocardial infarction (MI). Invariant natural killer T (iNKT) cells have been shown to produce inflammatory cytokines orchestrate tissue inflammation. However, no previous studies determined pathophysiological role iNKT post-MI LV remodeling.The purpose this study was examine whether activation might affect failure.After creation MI, mice received injection either...
Exercise capacity and quality of life are markedly impaired in chronic kidney disease (CKD). Increased plasma uremic toxins such as indoxyl sulfate (IS), which induce oxidative stress, may be involved this process. An oral adsorbent, AST-120, can reduce circulating IS, however, its effects on skeletal muscle exercise have not been investigated CKD. Subtotal-nephrectomy or sham operation was performed 8-week-old C57BL/6J mice. They were divided into two groups with without 8% (w/w) AST-120...
Abstract Systemic oxidative stress plays a key role in the development of chronic heart failure (CHF). We tested hypothesis that mitochondrial reactive oxygen species (ROS) generation circulating peripheral blood mononuclear cells (PBMCs) contributes to CHF progression. A total 31 patients who had history hospital admission due worsening HF were enrolled and grouped as having either mild defined New York Heart Association (NYHA) functional class I-II or moderate-to-severe NYHA III. ROS...
Abstract Invariant natural killer T (iNKT) cells, which bear αβ-type T-cell antigen-receptors (TCRs), recognize glycolipid antigens in a cluster of differentiation 1d (CD1d)-restricted manner. Regarding these the unique modes thymic selection and maturation elucidate innateness, irrespective them also being members adaptive immune system as T-cell. iNKT cells develop differentiate into NKT1 [interferon γ (IFN-γ)-producing], NKT2 [interleukin 4 (IL-4)/IL-13-producing], or NKT17...
Mouse allograft inflammatory factor-1 (AIF-1) cDNA was cloned and the AIF-1-specific monoclonal antibodies were established to examine its tissue distribution. The mouse AIF-1 highly conserved among all reported from a variety of species, invertebrates mammals, in good accordance with putative expressed regions genomic sequences major histocompatibility complex (MHC) class III region. messages abundantly testis, moderately spleen lymph nodes slightly liver thymus normal BALB/c mice....
▋ 3.3.1 Acute HF a. Organized Program to Initiate Lifesaving Treatment in Hospitalized Patients With Heart Failure (OPTIMIZE-HF) Risk Score OPTIMIZE-HF 56 is a model derived from large real-world registry that enrolled approximately 50,000 patients with acute the USA.Risk scores are calculated using 7 factors: age, systolic blood pressure, heart rate, serum creatinine, sodium, cause of hospitalization, and left ventricular ejection fraction (LVEF). b. Get The Guidelines-HF (GWTG-HF)...
Obesity and insulin resistance are associated with accelerated macrovascular microvascular coronary disease, cardiomyopathic phenomena, increased concentrations activity in blood of plasminogen activator inhibitor type 1 (PAI-1), the primary physiological fibrinolysis.To determine whether hypofibrinolysis tissues its potential sequelae could be attenuated pharmacologically, we studied genetically modified obese mice. By 10 weeks age, mice exhibited increases left ventricular weight glucose...
Objective— The C57BL/6 (B6) and 129 mouse inbred strains differ markedly in plasma HDL-cholesterol concentrations atherosclerosis susceptibility after a high-fat diet consumption. To identify loci controlling these traits, we performed quantitative trait (QTL) analysis. Methods Results— We fed to 294 (B6x129S1/SvImJ)F 2 females for 14 weeks, measured HDL size of aortic fatty-streak lesions, genotyped F females, QTL were affected by six loci: Hdlq14 Hdlq15 on chromosome 1 (peaks cM 80 104,...
The plasma lipid concentrations and obesity of C57BL/6J (B6) 129S1/SvImJ (129) inbred mouse strains fed a high-fat diet containing 15% dairy fat, 1% cholesterol, 0.5% cholic acid differ markedly. To identify the loci controlling these traits, we conducted quantitative trait (QTL) analysis 294 (B6 x 129) F(2) females for 14 weeks. Non-HDL cholesterol were affected by five significant loci: Nhdlq1 [chromosome 8, peak centimorgan (cM) 38, logarithm odds [LOD] 4.4); Nhdlq4 (chromosome 10, cM 70,...
Allograft inflammatory factor (AIF)-1, originally cloned from a rat heart allograft under chronic rejection, is induced in various conditions including atherosclerosis. Using mouse AIF-1 transfected macrophages and transgenic (AIF-1 Tg) mice, we analyzed the influence of overexpression on macrophage phagocytosis development The transfectants showed significantly increased latex beads E. coli BioParticles as well incorporation acetylated low-density lipoprotein (LDL) compared to those vector...
BMD is highly heritable; however, little known about the genes. To identify loci controlling BMD, we conducted a QTL analysis in (B6 x 129) F2 population of mice. We report on additional QTLs and also narrow one by combining data from multiple crosses through haplotype analysis.Previous studies have identified quantitative trait (QTL) that determine mice; identification genes underlying impeded large size regions.To performed 291 females. Total body vertebral areal (aBMD) were determined...
To identify genes controlling plasma HDL and triglyceride levels, quantitative trait locus (QTL) analysis was performed in one backcross, (NZO/H1Lt x NON/LtJ) NON/LtJ, three intercrosses, C57BL/6J DBA/2J, C3H/HeJ, NZB/B1NJ NZW/LacJ. concentrations were affected by 25 QTL distributed on most chromosomes (Chrs); those Chrs 1, 8, 12, 16 newly identified, the remainder replications of previously identified QTL. Triglyceride controlled nine loci; 2, 3, 7, 16, 18 QTL, replications. Combining mouse...
Plasminogen activator inhibitor-1 (PAI-1) inhibits fibrinolysis and proteolysis. Basic fibroblast growth factor (bFGF) stimulates angiogenesis, which requires regional Because modulation of vasculopathy tight control proteolysis, effects bFGF on PAI-1 expression in endothelial cells (ECs) were characterized. increased mRNA accumulation protein conditioned media human umbilical vein ECs. The bFGF-mediated increase was attenuated by inhibition extracellular signal-regulated kinase ECV304...
Intercrosses between inbred lines provide a traditional approach to analysis of polygenic inheritance in model organisms. Chromosome substitution strains (CSSs) have been developed as an alternative accelerate the pace gene identification quantitative trait mapping. We compared classical intercross and three CSS intercrosses examine genetic architecture underlying plasma high-density lipoprotein cholesterol (HDL) levels C57BL/6J (B) A/J (A) mouse strains. The B x A revealed significant loci...
Abstract Systemic inflammation underlies the association between obesity and nonalcoholic fatty liver disease (NAFLD). Here, we investigated functional changes in leukocytes’ mitochondria obese individuals their associations with NAFLD. We analyzed 14 male Japanese university students whose body mass index was > 30 kg/m 2 15 healthy age- sex-matched lean as controls. observed that mitochondrial oxidative phosphorylation (OXPHOS) capacity complex I + II-linked substrates peripheral blood...
Identification of genes that regulate BMD will enhance our understanding osteoporosis and could provide novel molecular targets for treatment or prevention. We generated a mouse intercross population carried out quantitative trait locus (QTL) analysis 143 female 124 male F(2) progeny from progenitor strains SM/J NZB/BlNJ using whole body vertebral areal (aBMD) as measured by DXA. found both aBMD was affected two loci on chromosome 9: one with significant epistatic interaction distal 8 the...
Lipopolysaccharide (LPS) has been shown to accelerate atherosclerosis and increase the prevalence of IL-4-producing natural killer T (NKT) cells in various tissues. However, role NKT development LPS-induced atherosclerotic lesions not fully tested cell-deficient mice. Here, we examined lesion apolipoprotein E knockout (apoE-KO) mice apoE-KO on an cell-deficient, CD1d (CD1d-KO) background (apoE-CD1d double knockout; DKO). LPS (0.5 μg/g body weight/wk) or phosphate-buffered saline (PBS) was...