A5054208401- Cardiovascular and exercise physiology
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Cardiovascular Function and Risk Factors
- Muscle metabolism and nutrition
- Diet and metabolism studies
- Renal cell carcinoma treatment
- Heart Rate Variability and Autonomic Control
- Sports Performance and Training
- Cancer Immunotherapy and Biomarkers
- Muscle Physiology and Disorders
- Heart Failure Treatment and Management
- Exercise and Physiological Responses
- Sperm and Testicular Function
- Hormonal and reproductive studies
- Prostate Cancer Treatment and Research
- Immune Cell Function and Interaction
- Sexual Differentiation and Disorders
- Bladder and Urothelial Cancer Treatments
- Urological Disorders and Treatments
- Cardiac Fibrosis and Remodeling
- Muscle activation and electromyography studies
- Reproductive Biology and Fertility
- Liver physiology and pathology
- ATP Synthase and ATPases Research
Osaka Police Hospital
2015-2025
Hokkaido University
2015-2024
Hokusho University
2009-2024
Keio University
2022
Osaka National Hospital
2018
Ikeda Municipal Hospital
2018
Osaka Rosai Hospital
2018
Osaka City General Hospital
2017
Osaka Prefectural Medical Center
2017
Hokkaido University of Education
2009-2016
Although recent studies have reported that low-intensity resistance training with blood flow restriction could stress the muscle effectively and provide rapid hypertrophy strength gain equivalent to those of high-intensity training, exact mechanism its generality not yet been clarified. We investigated intramuscular metabolism during exercise compared it exercises without using 31 P-magnetic resonance spectroscopy. Twenty-six healthy subjects (22 ± 4 yr) participated performed unilateral...
Our previous study reported that intramuscular metabolic stress during low-intensity resistance exercise was significantly enhanced by combining blood flow restriction (BFR); however, they did not reach the levels achieved high-intensity exercise. That performed using a single set of exercise; usual consists multiple sets with rest intervals. Therefore, we investigated multiple-set BFR exercises, and compared results those Twelve healthy young subjects 3 1-min unilateral plantar flexion (30...
Our previous study reported that metabolic stress in skeletal muscle achieved by combining moderate blood flow restriction (BFR) with low-intensity resistance exercise at 20% of one repetition maximum (1 RM) could not reach the level high-intensity exercise. Since protocol is typical current regimens this type, we sought to optimize for BFR examining dose effects intensity and pressure. Twelve healthy subjects participated study. They were asked perform unilateral plantar flexion 2 min (30...
Skeletal muscle bulk and strength are becoming important therapeutic targets in medicine. To increase mass, however, intensive, long-term mechanical stress must be applied to the muscles, such is often accompanied by orthopedic cardiovascular problems. We examined effects of circulatory occlusion resistance training combined with a very low-intensity load on enhancing muscular metabolic thereby increasing bulk. Muscular stress, as indicated increases inorganic phosphate (P(i)) decrease...
We have found that intestinal bacteria and their metabolites, short-chain fatty acids (SCFAs), promote cancer growth in prostate (PCa) mouse models. To clarify the association between gut microbiota PCa humans, we analyzed profiles of men with suspected PCa. One hundred fifty-two Japanese undergoing biopsies (96 56 without cancer) were included study randomly divided into two cohorts: a discovery cohort (114 samples) test (38 samples). The was compared groups, high-risk group (men Grade 2 or...
Abstract Background The pathophysiology of the prostate enlargement underlying lower urinary tract symptoms is unknown. Meanwhile, gut microbiota can contribute to various host conditions. We hypothesized that plays a role in enlargement. Methods included 128 patients who underwent biopsies at our hospitals between December 2018 and March 2020, excluding those had used antibiotics within past 6 months were diagnosed with cancer cT3 or higher. Patients volumes ≥30 ml defined as...
More than 70% of patients survive childhood leukemia, but chemotherapy and radiation therapy cause irreversible impairment spermatogenesis. Although autotransplantation germ cells holds promise for restoring fertility, contamination by leukemic may induce relapse. In this study, we isolated from mice FACS sorting. The cell population in the high forward-scatter low side-scatter regions dissociated testicular were analyzed staining MHC class I heavy chain (H-2K/H-2D) CD45. Cells that did not...
What is the central question of this study? We sought to examine whether curcumin could ameliorate skeletal muscle atrophy in diabetic mice by inhibiting protein ubiquitination, inflammatory cytokines and oxidative stress. main finding its importance? found that ameliorated streptozotocin-induced ubiquitination without affecting synthesis. This favourable effect was possibly due inhibition Curcumin may be beneficial for treatment type 1 diabetes mellitus. Skeletal develops patients with...
Chronic inflammation in the myocardium is involved development of left ventricular (LV) remodeling and failure after myocardial infarction (MI). Invariant natural killer T (iNKT) cells have been shown to produce inflammatory cytokines orchestrate tissue inflammation. However, no previous studies determined pathophysiological role iNKT post-MI LV remodeling.The purpose this study was examine whether activation might affect failure.After creation MI, mice received injection either...
What is the central question of this study? Does angiotensin II directly induce skeletal muscle abnormalities? main finding and its importance? Angiotensin induces abnormalities reduced exercise capacity. Mitochondrial dysfunction a decreased number oxidative fibres are manifest early, while atrophy seen later. Thus, may play an important role in observed wide variety diseases. Skeletal abnormalities, such as mitochondrial dysfunction, percentage atrophy, cause capacity ageing various...
Exercise capacity is reduced in heart failure (HF) patients, due mostly to skeletal muscle abnormalities including impaired energy metabolism, mitochondrial dysfunction, fibre type transition, and atrophy. Glucagon-like peptide-1 (GLP-1) has been shown improve exercise HF patients. We investigated the effects of administration a dipeptidyl peptidase (DPP)-4 inhibitor on an mouse model after myocardial infarction (MI).MI was created male C57BL/6J mice by ligating left coronary artery, sham...
Exercise capacity and quality of life are markedly impaired in chronic kidney disease (CKD). Increased plasma uremic toxins such as indoxyl sulfate (IS), which induce oxidative stress, may be involved this process. An oral adsorbent, AST-120, can reduce circulating IS, however, its effects on skeletal muscle exercise have not been investigated CKD. Subtotal-nephrectomy or sham operation was performed 8-week-old C57BL/6J mice. They were divided into two groups with without 8% (w/w) AST-120...
Here, we report on validating a mitochondrial gene therapy by delivering nucleic acids to mitochondria of diseased cells MITO-Porter, liposome-based carrier for delivery. We used derived from patient with disease G625A heteroplasmic mutation in the tRNAPhe DNA (mtDNA). It has been reported that some diseases are caused mutations, which both mutated and wild-type (WT) genes present, accumulation pathological mutations leads serious, intractable, multi-organ diseases. Therefore, decrease rate...
Abstract The environmental DNA (eDNA) technique is a convenient and powerful tool to detect rare species. Knowledge of the degradation rate eDNA in water important for understanding how influences presence and/or estimate biomass aquatic animals. We developed new set species-specific primers probe Japanese eel Anguilla japonica , which commercially endangered species, then conducted laboratory experiment quantify temperature-dependent emitted eDNA. Eels were held tanks at five different...
Decreased exercise capacity, which is an independent predictor of the poor prognosis patients with heart failure (HF), attributed to markedly impaired skeletal muscle mitochondrial function and fatty acid oxidation. Previous studies reported that administration inhibitor sodium-glucose cotransporter 2 (SGLT2) increases ketone body production fat utilization in type diabetic mice. In this study, we investigated effects SGLT2 on endurance oxidation a murine model HF after induction myocardial...
Heart failure (HF) is a leading cause of death and repeated hospitalizations often involves cardiac mitochondrial dysfunction. However, the underlying mechanisms largely remain elusive. Here, using mouse model in which myocardial infarction (MI) was induced by coronary artery ligation, we show metabolic basis dysfunction chronic HF. Four weeks after MI mice showed significant decrease succinyl-CoA levels, this impaired oxidative phosphorylation (OXPHOS) capacity. Heme synthesis ketolysis,...
More than 70% of patients survive childhood cancer, but chemotherapy and radiation therapy may cause irreversible impairment spermatogenesis. To treat infertility secondary to anticancer treatment for we have developed a procedure isolate germ cells from leukemic mice by fluorescence-activated cell sorting with two surface markers, transplantation isolated successfully restored fertility without inducing leukemia. In the present study, analyzed human malignant cells, including five leukemia...
Cross-coupling between bromoarenes and [(E)-CH3CH=CHCH2BF3]K (2a) by a catalyst prepared from Pd(OAc)2 D-t-BPF selectively provided γ-coupling products via SE2′ substitution. Mechanistic study of transmetalation revealed heretofore unknown process, namely, formation highly electrophilic [Pd(Ar)(D-t-BPF)]+ before with 2a. Thus, kinetic data in coupling 4-substituted 2a showed linear positive correlation (ρ = −1.1) accelerated donating substituents. This rate-determining role was further...
Systemic oxidative stress is associated with insulin resistance and obesity. We tested the hypothesis that systemic linked to lower aerobic capacity skeletal muscle dysfunction in metabolic syndrome (MetS).The incremental exercise testing cycle ergometer was performed 14 male patients MetS 13 age-, sex-, activity-matched healthy subjects. lipid peroxidation assessed by serum thiobarbituric acid reactive substances (TBARS), antioxidant defense total thiols enzymatic activity of superoxide...
Abstract Background Exercise intolerance is a common clinical feature and linked to poor prognosis in patients with heart failure (HF). Skeletal muscle dysfunction, including impaired energy metabolism the skeletal muscle, suspected play central role this intolerance, but underlying mechanisms remain elusive. Lysine acetylation, recently identified post‐translational modification, has emerged as major contributor derangement of mitochondrial metabolism. We thus investigated whether protein...
Abstract Systemic oxidative stress plays a key role in the development of chronic heart failure (CHF). We tested hypothesis that mitochondrial reactive oxygen species (ROS) generation circulating peripheral blood mononuclear cells (PBMCs) contributes to CHF progression. A total 31 patients who had history hospital admission due worsening HF were enrolled and grouped as having either mild defined New York Heart Association (NYHA) functional class I-II or moderate-to-severe NYHA III. ROS...
Combination therapy with immune checkpoint inhibitors has become the standard first-line treatment for metastatic renal cell carcinoma (mRCC), leading to changes in second-line options, such as nivolumab or tyrosine kinase (TKIs). However, very few studies have compared efficacy of these drugs patients mRCC, particularly those bone metastases (BM), which are associated a poor prognosis. This study and TKIs treatments mRCC BM examined microenvironments primary tumors lesions....