A5101616696- Sperm and Testicular Function
- Hormonal and reproductive studies
- Reproductive Biology and Fertility
- Sexual Differentiation and Disorders
- Urinary Bladder and Prostate Research
- Sexual function and dysfunction studies
- Testicular diseases and treatments
- Heat shock proteins research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Inhalation and Respiratory Drug Delivery
- Urologic and reproductive health conditions
- Prostate Cancer Diagnosis and Treatment
- Renal and related cancers
- Sexuality, Behavior, and Technology
- Protein Hydrolysis and Bioactive Peptides
- Hypothalamic control of reproductive hormones
- Bone health and osteoporosis research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Infant Nutrition and Health
- Urological Disorders and Treatments
- Bladder and Urothelial Cancer Treatments
- Evolutionary Psychology and Human Behavior
- Phytase and its Applications
- Ginger and Zingiberaceae research
Sumitomo Hospital
2019-2021
Jissen Women's University
2018
Kyoto University
2003-2014
Osaka University
2004-2013
Cedars-Sinai Medical Center
2009-2013
Osaka University of Pharmaceutical Sciences
2009
Emory University
2009
Coca Cola (United States)
2006
Microbial Chemistry Research Foundation
2004-2005
Megmilk Snow Brand (Japan)
1994-2002
We cloned a testis-specific cDNA from mice that encodes histone H1-like, haploid germ cell-specific nuclear protein designated HANP1/H1T2. The HANP1/H1T2 was specifically localized to the nuclei of murine spermatids during differentiation steps 5 13 but not mature sperm. contains an arginine-serine-rich domain and ATP/GTP binding site, it binds DNA, ATP, protamine. To investigate physiological role HANP1/H1T2, we generated Hanp1/H1T2-disrupted mutant mice. Homozygous Hanp1/H1T2 males were...
More than 70% of patients survive childhood cancer, but chemotherapy and radiation therapy may cause irreversible impairment spermatogenesis. To treat infertility secondary to anticancer treatment for we have developed a procedure isolate germ cells from leukemic mice by fluorescence-activated cell sorting with two surface markers, transplantation isolated successfully restored fertility without inducing leukemia. In the present study, analyzed human malignant cells, including five leukemia...
Little is known about the pathophysiology of acute exacerbation (AE) idiopathic pulmonary fibrosis (IPF). Heat shock protein 47 (HSP47), a collagen-specific molecular chaperone, essential for biosynthesis and secretion collagen molecules. Previous studies in experimental animal models have shown that downregulation HSP47 expression reduces production diminishes progression. In this study, serum levels were evaluated to elucidate pathogenic differences involving between AE-IPF stable (S)-IPF....
Milk contains several components effective for bone health. In the previous in vitro and vivo studies, we have shown that milk whey protein, especially its basic protein fraction (milk [MBP]), promoted formation suppressed resorption. This present study examines effect of MBP on biochemical markers metabolism healthy adult men. Experimental beverages containing (300 mg a day) were given to 30 normal men 16 days. The serum osteocalcin concentration had increased significantly after days...
ABSTRACT: Previously, we examined the relationship between protamine gene variations and human male infertility. In this study, show specific variability in transition nuclear protein genes (TNPs) of sterile patients. Transition proteins (TPs) are major that replace histones, leading to eventual substitution by protamines during spermiogenesis. Analysis TNP1 TNP2 sequences 282 patients 270 (TNP1) 266 (TNP2) proven‐fertile volunteers revealed 5 amino acid substitution‐causing single...
Hsp47 is a molecular chaperone that specifically recognizes procollagen in the endoplasmic reticulum. Hsp47-null mouse embryos produce immature type I collagen and form discontinuous basement membranes. We established Hsp47-/- embryonic stem cell lines examined formation of membrane production IV embryoid bodies, model for postimplantation egg-cylinder stage embryos. The visceral endodermal layers surrounding bodies were often disorganized, result suggested abnormal function under endoderm....
To investigate the possible association between variations in PRDM9 (MEISETZ) gene and impaired spermatogenesis humans, we screened for mutations human using DNA from 217 sterile male patients 162 proven fertile volunteers. Two single-nucleotide polymorphisms (SNPs), 17353G>T (Gly433Val) 18109C>G (Thr685Arg), were identified, as well an intronic SNP, 15549G>T. These SNPs identified heterozygous state separate who demonstrated azoospermia. Neither variant was subjects. Our results suggest...
We evaluated the morphological effect and alterations in gene expression caused by 1,25-dihydroxyvitamin D treatment mouse testis undergoing experimental cryptorchidism subsequent orchiopexy.The mean modified Johnsen score testicular weight were estimated after 4 weeks of with a prodrug. examined sites vitamin receptor mRNA expression, analogue accumulation testis. Also, we compared cryptorchid or without administration specific cDNA microarray. confirmed protein synthesis candidate among...
In this study, we investigated whether dietary glucosylceramide (GlcCer) and its metabolite sphingoid bases, sphingosine (SS), phytosphingosine (PS), sphingadienine (SD) 4-hydroxysphingenine (4HS), influence cornified envelope (CE) formation. CE is formed during terminal differentiation of the epidermis through crosslinking specific precursor proteins by transglutaminases (TGases), essential for skin's barrier function. Oral administration GlcCer (0.25 mg/day) 14 consecutive days...
Symptoms of partial androgen deficiency the aging male (PADAM), such as sexual dysfunction and depression, are receiving increased attention. Currently bioavailable testosterone (BT) is considered most reliable marker for establishing presence hypogonadism. We clarified relationship between BT other hormones with respect to patient age PADAM symptoms.A total 130 patients who visited our special clinics function were included in this study. Endocrinological profiles evaluated appropriate,...
Background The development of novel fertilization treatments, including in vitro and intracytoplasmic injection, has made pregnancy possible regardless the level activity spermatozoa; however, etiology male-factor infertility is poorly understood. Multiple studies, primarily through use transgenic animals, have contributed to a list candidate genes that may affect male humans. We examined single nucleotide polymorphisms (SNPs) as cause an analysis spermatogenesis-specific genes. Methods...
We studied the effects of milk basic protein (MBP) on bone metabolism in ovariectomized (OVX) rats. Five-week-old female Sprague-Dawley rats were and fed a low-calcium diet (0.009% Ca) for 5 weeks. The OVX divided into three experimental groups: Control group (20% casein), MBP-L (19.9% casein, 0.1% MBP), MBP-H (19% 1% MBP) six animals. each 3 breaking strength energy femur significantly higher than those control group. was also There no differences amount femoral calcium phosphorus among...
A cystein protease inhibitor was identified in the basic fraction of bovine milk. We have reported our previous study that milk protein (MBP) suppressed osteoclast-mediated bone resorption vitro. Since osteoclasts secreted to digest collagen matrix, we MBP. 12-kDa purified from MBP by papain affinity gel chromatography and subsequent Hi-Load Superdex 75 filtration chromatography. The N-terminal sequence 18 amino acid residues corresponded cystatin C. therefore seemed be Purified with use...
Penile erection is dependent on commands from the central nervous system. Although basic studies of animals and neuroimaging humans have been conducted to identify key brain regions associated with sexual arousal, our knowledge no reliable first excitation phase arousal leading penile reported.We used H(2)(15)O-positron emission tomography analyze regional cerebral blood flow just before in heterosexual volunteers. The subjects viewed 3 different types audiovisual materials-sexually explicit...
To evaluate the early efficacy of alpha(1A)-adrenoceptor selective drug, silodosin, for treatment lower urinary tract symptoms suggestive benign prostatic hyperplasia.A total 68 patients with an International Prostate Symptom Score (IPSS) >==8 and a Quality Life (QOL) index >==2 were included. Changes in IPSS QOL evaluated before after 1, 2, 3, 4, 5, 6, 7, 14, 28 days twice daily oral administration 4 mg silodosin. Next, changes subscores as well voiding, storage, post micturition assessed....
Bone metastasis is the most lethal form of several cancers. The β2-microglobulin (β2-M)/hemochromatosis (HFE) complex plays an important role in cancer development and bone metastasis. We demonstrated previously that overexpression β2-M prostate, breast, lung renal leads to increased mouse models. Therefore, we hypothesized a rational target treat prostate metastasis.In this study, demonstrate its binding partner, HFE, modulating radiation sensitivity chemo-sensitivity cancer. By genetic...
Recently we cloned the Hanp1 cDNA that encodes a histone H1-like haploid germ cell-specific nuclear protein in mouse. Homozygous mutant male mice were infertile, while females fertile. Although substantial number of sperm recovered from epididymis, their shape and function abnormal. is essential for formation functional spermatozoa, specifically involved replacement histones with protamines during spermiogenesis. To investigate roles human HANP1 (h-HANP1) its relation to infertility,...