A5072709086- Bladder and Urothelial Cancer Treatments
- Urinary and Genital Oncology Studies
- Prostate Cancer Treatment and Research
- Renal cell carcinoma treatment
- Cancer Immunotherapy and Biomarkers
- Prostate Cancer Diagnosis and Treatment
- Gut microbiota and health
- Cancer, Lipids, and Metabolism
- Renal and related cancers
- Diet and metabolism studies
- Sperm and Testicular Function
- Urinary Bladder and Prostate Research
- Cancer Genomics and Diagnostics
- Inflammatory Biomarkers in Disease Prognosis
- MicroRNA in disease regulation
- Urological Disorders and Treatments
- Extracellular vesicles in disease
- Testicular diseases and treatments
- Hormonal and reproductive studies
- Cancer-related molecular mechanisms research
- Radiopharmaceutical Chemistry and Applications
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Urologic and reproductive health conditions
- Immunotherapy and Immune Responses
Kindai University
2020-2025
Kindai University Hospital
2021-2025
Osaka University
2015-2024
Lawrence Berkeley National Laboratory
2019-2024
Soai University
2022-2024
Kanagawa Children's Medical Center
2002-2021
Tokyo Metropolitan Children's Medical Center
2011-2019
Suita Municipal Hospital
2018
Japanese Red Cross Kobe Hospital
2018
Red Cross Hospital
2018
Excessive intake of animal fat and resultant obesity are major risk factors for prostate cancer. Because the composition gut microbiota is known to change with dietary body type, we used prostate-specific Pten knockout mice as a cancer model investigate whether there microbiota-mediated connection between Oral administration an antibiotic mixture (Abx) in cancer-bearing fed high-fat diet containing large proportion lard drastically altered including Rikenellaceae Clostridiales, inhibited...
We determined whether serial prostate needle biopsies predispose men to erectile dysfunction and/or lower urinary tract symptoms over time.Men with cancer on an active surveillance protocol were administered the 5-item Sexual Health Inventory for Men and International Prostate Symptom Score questionnaires entry, at a cross-sectional point in 2008. All had least 1, 10 12-core biopsy entry yearly thereafter recommended.Of 333 231 returned followup questionnaires. Correlations found between...
Abstract Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. were isolated urine after massage 18 men (negative biopsy [n = 6], GS 6 PCa or 8–9 6]). EV proteins labeled with iTRAQ and analyzed by LC-MS/MS. identified 4710 quantified 3528 the EVs. Eleven increased patients compared those negative (ratio >1.5, p-value < 0.05)....
Current non-invasive approaches for detection of urothelial cancers are suboptimal. We developed a test to detect neoplasms using DNA recovered from cells shed into urine. UroSEEK incorporates massive parallel sequencing assays mutations in 11 genes and copy number changes on 39 chromosome arms. In 570 patients at risk bladder cancer (BC), was positive 83% those who BC. Combined with cytology, detected 95% Of 56 upper tract cancer, 75% tested by UroSEEK, including 79% tumors. genetic...
Purpose: High-fat diet (HFD) could induce prostate cancer progression. The aim of this study is to identify mechanisms HFD-induced progression, focusing on inflammation.Experimental Design: We administered HFD and celecoxib autochthonous immunocompetent Pb-Cre+;Pten(fl/fl) model mice for cancer. Tumor growth was evaluated by tumor weight Ki67 stain, local immune cells were assessed flow cytometry at 22 weeks age. Cytokines which correlated with identified, the changes after inhibition...
Abstract Bladder cancer causes an estimated 150,000 deaths per year worldwide. Although 15% of the recurrent bladder becomes invasive type, currently used targeted therapy for malignant is still not efficient. We focused on miR-130 family (miR-130b, miR-301a and miR-301b) that was significantly upregulated in specimens than normal urothelial specimens. analyzed functional significance using a 5637 cell line revealed inhibitors suppressed migration invasion by downregulating focal adhesion...
// Kyosuke Matsuzaki 1 , Kazutoshi Fujita Kentaro Jingushi 2 Atsunari Kawashima Takeshi Ujike Akira Nagahara Yuko Ueda Go Tanigawa 3 Iwao Yoshioka 4 Koji 5 Rikinari Hanayama 6 Motohide Uemura Yasushi Miyagawa Kazutake Tsujikawa Norio Nonomura Department of Urology, Osaka University Graduate School Medicine, Osaka, Japan Laboratory Molecular and Cellular Physiology, Pharmaceutical Science, General Medical Center, Police Hospital, Cancer Proteomics Group, Genome Japanese Foundation for...
We have found that intestinal bacteria and their metabolites, short-chain fatty acids (SCFAs), promote cancer growth in prostate (PCa) mouse models. To clarify the association between gut microbiota PCa humans, we analyzed profiles of men with suspected PCa. One hundred fifty-two Japanese undergoing biopsies (96 56 without cancer) were included study randomly divided into two cohorts: a discovery cohort (114 samples) test (38 samples). The was compared groups, high-risk group (men Grade 2 or...
Abstract Background The pathophysiology of the prostate enlargement underlying lower urinary tract symptoms is unknown. Meanwhile, gut microbiota can contribute to various host conditions. We hypothesized that plays a role in enlargement. Methods included 128 patients who underwent biopsies at our hospitals between December 2018 and March 2020, excluding those had used antibiotics within past 6 months were diagnosed with cancer cT3 or higher. Patients volumes ≥30 ml defined as...
Abstract Objectives Enfortumab vedotin (EV) is a novel antibody–drug conjugate approved for metastatic urothelial carcinoma (UC) refractory to prior treatment with immune checkpoint inhibitors (ICIs). However, the difference in efficacy of EV after each ICIs and prognostic factors are not well known. We aimed compare patients UC who were treated avelumab or pembrolizumab identify factors. Methods The records 100 advanced received administration either retrospectively collected from five...
More than 70% of patients survive childhood leukemia, but chemotherapy and radiation therapy cause irreversible impairment spermatogenesis. Although autotransplantation germ cells holds promise for restoring fertility, contamination by leukemic may induce relapse. In this study, we isolated from mice FACS sorting. The cell population in the high forward-scatter low side-scatter regions dissociated testicular were analyzed staining MHC class I heavy chain (H-2K/H-2D) CD45. Cells that did not...
Microsurgical techniques in testicular sperm extraction can improve retrieval patients with nonobstructive azoospermia (NOA). However, spermatozoa rates have still been reported to be around 50% for NOA. Thus, a reliable prediction method successful outcome is needed avoid unnecessary surgery. In this retrospective study we determined the diagnostic and predictive values of noninvasive parameters used treatment NOA.We analyzed 9 preoperative factors including patient age, volume...
Reliable biomarkers for renal cell carcinoma (RCC) have yet to be determined. Circulating tumor DNA (ctDNA) is an emerging resource detect and monitor molecular characteristics of various tumors. The present study aims clarify the clinical utility ctDNA RCC. Fifty‐three patients histologically diagnosed with clear RCC were enrolled. Targeted sequencing was carried out using plasma cell‐free (cfDNA) DNA. We applied droplet digital PCR (ddPCR) validate detected mutations. cfDNA fragment size...
Cancer‐associated extracellular vesicles (EVs) are intimately involved in establishment of tumor microenvironment and occurrence metastasis. However, previous studies have mainly relied on experiments with cultured cell lines or mouse models, making it difficult to gain a full understanding EV functions human body. Hence, we extracted EVs directly from surgically resected viable clear renal carcinoma (ccRCC) tissues adjacent normal ( n = 20). Quantitative LC/MS analysis identified 3,871...
Abstract Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney, and clear RCC (ccRCC) represents its histological subtype. To identify a therapeutic target for ccRCC, miRNA expression signatures from ccRCC clinical specimens were analyzed. microarray real-time PCR analyses revealed that miR-629 was significantly upregulated in human compared with adjacent noncancerous renal tissue. Functional inhibition by hairpin inhibitor suppressed motility invasion. Mechanistically,...
Most upper tract urothelial carcinomas ( UTUC ) are muscle invasive at the time of diagnosis. Current standard methods for diagnosis invasive. Urine cytology is only non‐invasive test detecting , but its sensitivity low. A novel assay detection would improve patient outcome. This study aimed to investigate mutation cell‐free DNA (cf in urine supernatant develop a reliable diagnostic biomarker patients. We studied urinary cf from 153 individuals, including 56 patients with localized and...
Enfortumab vedotin is a novel antibody-drug conjugate targeting Nectin-4, which highly expressed in urothelial carcinoma. However, the expression status of Nectin-4 upper tract carcinoma (UTUC) remains unclear. The relationship between and Programmed Death Ligand 1 (PD-L1) UTUC also ambiguous. We performed immunohistochemical analysis 99 tissue microarray to assess PD-L1 UTUC. Nectin-4-positivity was detected 65 (65.7%) samples, 24 (24.2%) samples. There no correlation PD-L1. Patients with...