A5034211195- Bladder and Urothelial Cancer Treatments
- Renal cell carcinoma treatment
- Cancer Immunotherapy and Biomarkers
- Prostate Cancer Treatment and Research
- Urinary and Genital Oncology Studies
- Gut microbiota and health
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- Cancer, Lipids, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Diet and metabolism studies
- Immunotherapy and Immune Responses
- Inflammatory Biomarkers in Disease Prognosis
- Cancer, Stress, Anesthesia, and Immune Response
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Immune cells in cancer
- Multiple and Secondary Primary Cancers
- Nutrition, Genetics, and Disease
- Cancer Diagnosis and Treatment
- Bone health and treatments
- Sarcoma Diagnosis and Treatment
- Cancer Research and Treatments
Osaka University
2016-2025
Queen's University
2013-2023
Soai University
2022
Kindai University
2022
Suita Municipal Hospital
2018
Laboratoire d'études sur les monothéismes
2016
Soka University
2010-2011
Osaka Medical Center for Cancer and Cardiovascular Diseases
2010
Sumitomo Hospital
2010
Osaka Rosai Hospital
2008-2009
Excessive intake of animal fat and resultant obesity are major risk factors for prostate cancer. Because the composition gut microbiota is known to change with dietary body type, we used prostate-specific Pten knockout mice as a cancer model investigate whether there microbiota-mediated connection between Oral administration an antibiotic mixture (Abx) in cancer-bearing fed high-fat diet containing large proportion lard drastically altered including Rikenellaceae Clostridiales, inhibited...
Foxp3-expressing CD25+CD4+ regulatory T cells (Tregs) are abundant in tumor tissues. Here, hypothesizing that Tregs would clonally expand after they activated by tumor-associated antigens to suppress antitumor immune responses, we performed single-cell analysis on characterize them cell receptor clonotype and gene-expression profiles. We found multiclonal present tissues predominantly expressed the chemokine CCR8. In mice humans, CCR8+ constituted 30 80% of various cancers less than 10%...
Study Type – Prognostic (case series) Level of Evidence 4 What’s known on the subject? and What does study add? In general, tumour‐associated macrophage (TAM) is important for tumour progression including prostate cancer. this paper, we clarified usefulness TAM in biopsy specimens predicting prognosis after hormonal therapy. OBJECTIVE • To evaluate infiltration as a possible prognostic factor cancer (PCa) PATIENTS AND METHODS Immunostaining TAMs was performed using monoclonal antibody CD68...
Abstract Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. were isolated urine after massage 18 men (negative biopsy [n = 6], GS 6 PCa or 8–9 6]). EV proteins labeled with iTRAQ and analyzed by LC-MS/MS. identified 4710 quantified 3528 the EVs. Eleven increased patients compared those negative (ratio >1.5, p-value < 0.05)....
Purpose: High-fat diet (HFD) could induce prostate cancer progression. The aim of this study is to identify mechanisms HFD-induced progression, focusing on inflammation.Experimental Design: We administered HFD and celecoxib autochthonous immunocompetent Pb-Cre+;Pten(fl/fl) model mice for cancer. Tumor growth was evaluated by tumor weight Ki67 stain, local immune cells were assessed flow cytometry at 22 weeks age. Cytokines which correlated with identified, the changes after inhibition...
Regulatory T cells (Tregs) have an immunosuppressive role in the tumor microenvironment. Since effector Tregs (eTregs), which highly suppressive functions, are located a subpopulation of Foxp3+ CD4+ Tregs, TCR-inducible costimulatory receptor (ICOS) was applied as marker eTregs that infiltrated gastric cancer tissue and induction pathway ICOS+ analyzed by flow cytometry immunohistochemistry. In tumor-infiltrating lymphocytes (TILs), were abundantly observed late stages cancer. TILs exhibited...
// Kyosuke Matsuzaki 1 , Kazutoshi Fujita Kentaro Jingushi 2 Atsunari Kawashima Takeshi Ujike Akira Nagahara Yuko Ueda Go Tanigawa 3 Iwao Yoshioka 4 Koji 5 Rikinari Hanayama 6 Motohide Uemura Yasushi Miyagawa Kazutake Tsujikawa Norio Nonomura Department of Urology, Osaka University Graduate School Medicine, Osaka, Japan Laboratory Molecular and Cellular Physiology, Pharmaceutical Science, General Medical Center, Police Hospital, Cancer Proteomics Group, Genome Japanese Foundation for...
Reliable biomarkers for renal cell carcinoma (RCC) have yet to be determined. Circulating tumor DNA (ctDNA) is an emerging resource detect and monitor molecular characteristics of various tumors. The present study aims clarify the clinical utility ctDNA RCC. Fifty‐three patients histologically diagnosed with clear RCC were enrolled. Targeted sequencing was carried out using plasma cell‐free (cfDNA) DNA. We applied droplet digital PCR (ddPCR) validate detected mutations. cfDNA fragment size...
Most upper tract urothelial carcinomas ( UTUC ) are muscle invasive at the time of diagnosis. Current standard methods for diagnosis invasive. Urine cytology is only non‐invasive test detecting , but its sensitivity low. A novel assay detection would improve patient outcome. This study aimed to investigate mutation cell‐free DNA (cf in urine supernatant develop a reliable diagnostic biomarker patients. We studied urinary cf from 153 individuals, including 56 patients with localized and...
Enfortumab vedotin is a novel antibody-drug conjugate targeting Nectin-4, which highly expressed in urothelial carcinoma. However, the expression status of Nectin-4 upper tract carcinoma (UTUC) remains unclear. The relationship between and Programmed Death Ligand 1 (PD-L1) UTUC also ambiguous. We performed immunohistochemical analysis 99 tissue microarray to assess PD-L1 UTUC. Nectin-4-positivity was detected 65 (65.7%) samples, 24 (24.2%) samples. There no correlation PD-L1. Patients with...
Abstract Proteomic analysis of urinary extracellular vesicles (EVs) is a powerful approach to discover potential bladder cancer (BCa) biomarkers, however urine contains numerous EVs derived from the kidney and normal urothelial epithelium, which can obfuscate information related BCa cell‐derived EVs. In this study, we combined proteomic tissue‐exudative (Te‐EVs), were isolated culture medium freshly resected viable tissues. Urinary samples 11 individuals (7 patients 4 healthy individuals),...
Abstract Regulatory T cells (Tregs) suppress the host immune response and maintain homeostasis. Tregs also promote cancer progression are involved in resistance to checkpoint inhibitor treatments. Recent studies identified selective CCR8 expression on tumor-infiltrating Tregs; CCR8+ have been indicated as a possible new target of immunotherapy. Here, we investigated features lung patients. were highly activated infiltration tumors was associated with poor prognosis We their suppressive...
Bacterial flora are present in various parts of the human body, including intestine, and thought to be involved etiology diseases such as multiple sclerosis, intestinal diseases, cancer, uterine diseases. In recent years, presence bacterial 16S rRNA genes has been revealed blood, which was previously a sterile environment, characteristic blood microbiomes have detected However, mechanism origin information unknown. this study, we performed metagenomic analysis DNA serum extracellular...
Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource the diagnosis and prognosis of various cancers. This study aims identify novel blood RCC.Plasma cfDNA was extracted from RCC patients (n = 92) healthy controls 41). Levels were determined using quantitative real-time PCR ACTB as target gene, fragment size measured a microfluidics-based platform. Diagnostic potential assessed receiver operating characteristic...
Abstract Cancer immunotherapy, including immune checkpoint inhibitors, exerts beneficial effects in cancer patients. However, inhibitors are only advantageous for a limited population of Therefore, companion diagnostics needed order to identify patients whom these therapies effective. In the present study, we evaluated detailed immunological aspects clinical specimens from non-small cell lung (NSCLC) We analyzed profiles, T cytotoxicity, and TCR repertoire peripheral blood, normal tissue,...
Extracellular vesicles (EVs) including exosomes are present in blood, urine, and saliva contain proteins, microRNAs, messenger RNAs. We investigated microRNAs urinary EVs to discover new biomarkers of prostate cancer (PCa).We isolated from urine obtained following digital rectal examination (DRE) 14 men with elevated levels serum prostate-specific antigen (PSA) [negative biopsy (n=4) PCa Gleason scores 6 (n=3), 7 8-9 (n=4)]. MicroRNAs extracted were analyzed by microRNA microarray.MicroRNAs...
Abstract Western high‐fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa). Using prostate‐specific Pten ‐knockout mice PCa model, we previously reported that HFD promoted inflammatory growth. The composition of the gut microbiota changes under influence diet exert various effects on host through immunological mechanisms. Herein, investigated etiology HFD‐induced growth and involvement microbiome. expression Hdc , gene responsible histamine biosynthesis, levels were...
Combination therapy with immune checkpoint inhibitors has become the standard first-line treatment for metastatic renal cell carcinoma (mRCC), leading to changes in second-line options, such as nivolumab or tyrosine kinase (TKIs). However, very few studies have compared efficacy of these drugs patients mRCC, particularly those bone metastases (BM), which are associated a poor prognosis. This study and TKIs treatments mRCC BM examined microenvironments primary tumors lesions....
We have reported that the FAN score, which consists of fibrosis-4 (Fib-4) index, albumin-bilirubin (ALBI) and neutrophil-lymphocyte ratio (NLR), is a prognostic marker for patients receiving pembrolizumab mUC. However, it remains unclear whether score factor platinum-based first-line chemotherapy. included 126 with mUC who received The median PFS overall survival (OS) were 6.3 months (95% confidence interval [CI] 4.5-8.0) 14.0 CI 11.1-16.9), respectively. Multivariable analysis showed Fib-4...
Chemoradiation therapy (CRT) is now widely recognized as bladder-preserving for muscle-invasive bladder cancer (MIBC). However, some patients who fail CRT may miss the chance to be cured by cystectomy. Therefore, it important select with MIBC are expected have a good response CRT. Several reports indicate that excision repair cross-complementing group 1 (ERCC1) gene associated resistance cisplatin and radiation therapy. In this study, we examined correlation between ERCC1 in vitro vivo...
Extracellular matrix metalloproteinase inducer (EMMPRIN) has been reported to play crucial roles, including in angiogenesis, several carcinomas. However, the correlation between EMMPRIN levels and angiogenesis expression profile not reported, role of renal cell carcinoma (RCC) is unclear. In present study, we evaluated association with its value prognosis, roles RCC.EMMPRIN was examined 50 RCC patients treated radical nephrectomy. Angiogenesis, proliferation, invasion activity were using...
Systemic inflammation and immune responses are reported to be associated with progressive prostate cancer. In this study, we explored which among the fractions of white blood cell (WBC) C-reactive protein (CRP) level were high Gleason score Prostate needle biopsy was performed in 966 men suspicion We assessed age, serum prostate-specific antigen (PSA), volume, WBC count, WBCs (neutrophils, lymphocytes, monocytes, basophils, eosinophils), CRP before for associations findings. Among all men,...